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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Myc will climb the walls regardless. Oyster myc will bust out of a plate.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Hidden contams [Re: maddchef]
#23178362 - 05/01/16 11:29 PM (7 years, 8 months ago) |
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whew, thanks. That's a relief!
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 17 hours, 25 minutes
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Mycelium normally does colonize the sides if you let it. The extent to which it seems to vary but it's not related to bacteria. You should try not let it colonize to that point though since it can grow into a contaminated part of the petri.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Hidden contams [Re: Kizzle]
#23178687 - 05/02/16 02:31 AM (7 years, 8 months ago) |
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Quote:
Kizzle said:
Quote:
Supalemonhaze said: Like the one below. Some parts it's organised and beautiful yet all around it is all this strange growth. Strange thing is, the sector I took that transfer from was totally rhizo with no cottony growth at all.

There are others which I took transfers from nice rhizomorphic growth but they have a hard time staying above the surface and growth is all strange.

I tried to take clear pics of the growth under the surface the other day, this is the best I could come up with.
 
Sometimes that just happens, especially when transferring to a different agar mix. Keep transferring until you get a monoculture though. It's the best way to help ensure it's pure.
Thanks for the re-assurance. I am still isolating it, I'll use the plates I took transfers from on grains to see what they do as well.
Pretty sure I used a shitload of spores in that inoculation, this is taking forever . God help me if I isolated and kept all the strains, I would have well over a hundred, at least. I am trying to choose around 20ish of the most good looking and rhizomorphic ones, that should give me a good isolate I reckon.
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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 3 months
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This is one of the most important and useful threads I've come across. Many thanks!
Marking for reference.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,064
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-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 3 hours, 26 minutes
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I need to start doing proper transfers, just got 100 dishes so ill start in the morning. If anybodys interested in yeasts for brewing you can collect the yeasts from the air with an open container over a few weeks the traditional way. I have some mead made this way thats got some dried cherries soaking in it meow. Also should you buy some beer you enjoy, you can collect it's yeast from the bottom of the bottles if you poured em right. Just did this the other day drom magic hats ticket to rye.
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pacmanbreed


Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
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Re: Hidden contams [Re: Failboat]
#25015574 - 02/23/18 09:48 AM (5 years, 11 months ago) |
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Mycogone hard to spot before it matures. Damn have spend hundreds of petri. Can you still isolate once it hits?
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plurfekt
Finally Grateful


Registered: 12/14/09
Posts: 1,919
Loc: USA
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Quote:
pacmanbreed said: Mycogone hard to spot before it matures. Damn have spend hundreds of petri. Can you still isolate once it hits?
So I'm not the only one.. XD
Yea, it's a motherfucker. It basically pretends to be healthy mycelium right up until pinning. I've tossed most of my plates and am starting new ones from spore while trying to isolate healthy tissue off the most promising ones remaining.
Lost a bunch of plates, two tubs, one LC, and counting.
Also have 14 quarts that could easily be contaminated with it and there would be no way to know until fruit.
When in doubt...toss it out. But man, that was an entire bag of Pennington Classic and 5 hours of grain prep. Not man about the few $, but my few hours... 
I'm currently reading Mad Season's thread on hidden contaminants (very helpful) and figuring out if I need a steam cleaner/flowhood or not... lol
Definitely going to revise my techniques once again and if nothing else, this is some seriously good practice/experience.
I didn't even know there was a mold that could bruise blue and hide up until fruiting. Murphy's law...
Now, onto pouring plates and isolating healthy tissue. Only been doing agar a few months, and this was the only contamination I've seen out of all of my plates.
Appreciate the effort put into this thread to help others.
Here's some info on mycogone I found off google:
Mycogone. This fungal disease is transmitted primarily by contaminated casing. It infects the pins or mushroom initials as they start to form but symptoms do not appear for 10-14 days. As you say - by then it is too late. The best defense is to make sure the casing is well steamed prior to application to the beds. If necessary, treatment of the casing with 1% formalin can be used if this is legal where you are growing. Treatment of the casing with benomyl, carbendazim, prochloraz manganese or thiabendazole can also be effective. If this is a chronic problem on the farm, I suggest application of these fungicides as a preventative to the casing at every crop. There has been no reported resistance to the benzimadazole fungicides but rotation of these chemicals is always a good practice if possible. Many times Mycogone is seasonal so if you have a season of the year (typically drier) when Mycogone is not so prevalent you can suspend the fungicide treatments during those months. Sanitation and hygiene is of utmost importance and must be part of farm management year round. . If you find diseased mushrooms, weeping caps, distorted shapes, infected stipes - these mushrooms must be picked off by your best pickers wearing gloves before watering or harvest and disposed of off the farm. Watering is an important way the spores of this fungus are spread. Infected casing / compost should be isolated with heavy applications of salt or by pushing a plastic pot deep into the compost casing. Steaming after the crop has been harvested is very important to eliminate this threat from the farm. The spores can become air-borne so this must be done before cleanout.
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pacmanbreed



Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
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Re: Hidden contams [Re: plurfekt]
#25300228 - 06/30/18 05:13 AM (5 years, 6 months ago) |
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Yes mycogone can be a pain in the ass. But it can be sometimes a false positive under a scope acording to bhod.
If my memory serves me right. I think ive grown fruits out of those plate once ive said fukit, ill try it on grains and shoeboxes.
I still wonder if i really had this contam which bothered me for months or is it something else eg. Due to Highend temp, a mutation or some bacterial response.
Hope we can identify this in the future. Will try to recreate this if i can produced fruits if i was hit by that mycogone looking plate nexttime around.
Mycogone Perniciosa AKA "wet bubble
Edited by pacmanbreed (06/30/18 05:17 AM)
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plurfekt
Finally Grateful


Registered: 12/14/09
Posts: 1,919
Loc: USA
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Due to lack of a microscope and unnecessary debate over whether or not TC's are right in all cases, or Bod is right in -one- case, I've pretty much stopped giving a shit as I destroyed anything that could possibly contain mycogone, and bleached all my tubs/gave them a cool water bath afterwards.
Based on what I've read steam cleaning is the way to go if mycogone ever reaches fruiting stage (which if mine was mycogone, it did), as well as certain pesticides in commercial applications for farming to prevent future rashes. The best budget steam cleaner is about 140$ on the zon' and I figured it is a solid investment for a clean freak looking to rule out vectors when I relocate.
Not a bad idea for people who live in old houses/trailers that have a really high spore load, as I've been looking at places in the country, they are common.
One clean tub does not mean that every TC has been wrong. Also stand by not feeding that nasty shit to anyone...
Whatever, people gonna do what people gonna do
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 3 hours, 26 minutes
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Re: Hidden contams [Re: plurfekt]
#25300906 - 06/30/18 01:26 PM (5 years, 6 months ago) |
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Steam cleaner, nice. I should get one.
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bugman
buggy


Registered: 05/10/15
Posts: 245
Loc: united kingdom
Last seen: 4 months, 20 days
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Re: Hidden contams [Re: Kizzle]
#25306100 - 07/03/18 09:04 AM (5 years, 6 months ago) |
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Quote:
Kizzle said: Here's one with hidden Aspergillus.

This is a clone of one of the agar pins.

Quote:
Plus they don't need a pc cycle.
Glass petris should really be oven sterilized anyway.
I dont see the contam in the first pic can do.eone help me out here
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pacmanbreed



Registered: 10/12/16
Posts: 3,659
Last seen: 1 year, 11 months
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Re: Hidden contams [Re: bugman]
#25306847 - 07/03/18 05:32 PM (5 years, 6 months ago) |
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Its hidden in the mycelium mat it migth be on 8oclock outer edge of the dish, The mat look growth. You can see em if you look @ dishes under a light and backlit from my exp.
Edited by pacmanbreed (07/03/18 05:36 PM)
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Dr3
Stranger


Registered: 05/23/19
Posts: 96
Last seen: 11 months, 16 days
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I'm getting these white spots on 3 week old plates. Are they primordia or something like aspergillus? Thanks

so I presume that wispy thing on the borders is mold, right?
The plate turn into this 
Edited by Dr3 (03/04/20 05:14 PM)
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polko
Stranger

Registered: 06/21/20
Posts: 15
Last seen: 2 months, 25 days
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Good info! Thanks man! Beginners appreciate it!
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Stevie Ray Spawn
Stranger Danger


Registered: 11/26/21
Posts: 2
Loc: Bucks County Pa
Last seen: 1 year, 8 months
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 Lurking newb here, trying to read as much as possible but this site is so dense with info It would take year to read it all! ha
I have some GT LC into Pc'd Millet from 3/12. I'm ready to dump into Bod's monotub coir substrate.
I'm nt sure if my grain spawn is contaminated. I shook up last week.
I have 7 qts, 1 pic each, any thoughts from the experts? I have trouble telling contamination from non-contamination, 90 days into this journey.
 
-------------------- IN 70 days I went from Spray Kits to grain on substrate to Liquid Culture in PC'd Millet to spore swab on agar, grow tents, fans, heaters, CO2 monitors, lights, wifi controllers. I expect to be talking to aliens by December!
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