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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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 added to op. Thanks :P
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
This right here is why I never say that I don't need to improve my techniques, or that I'm 100% clean. I'm not, and I'm constantly working on my sterile procedures.
i am too. i hope we all are. some people say they don't get con tams.
pasty said it best once, i can't remember exactly how he put it, but it was something like
"if you say you don't get contaminants, u are either lying, or ur not growing enough"
that is so true. it happens to us all. sometimes in long streaks, sometimes short ones. sometimes just here and there. we are growing mushrooms indoors, in make shift clean rooms. mold loves to hide in small crevices. it happens to the best of us, and no one should be ashamed of it. that kind of pride will be a persons downfall and should be avoided at all costs. lets be real. its ok, it doesn't make you a bad person, or a bad grower. usually, the opposite is true, and it will make you a better one if ur observant enough about the situation.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Quote:
blindingleaf said:
Quote:
This right here is why I never say that I don't need to improve my techniques, or that I'm 100% clean. I'm not, and I'm constantly working on my sterile procedures.
i am too. i hope we all are. some people say they don't get con tams.
pasty said it best once, i can't remember exactly how he put it, but it was something like
"if you say you don't get contaminants, u are either lying, or ur not growing enough"
that is so true. it happens to us all. sometimes in long streaks, sometimes short ones. sometimes just here and there. we are growing mushrooms indoors, in make shift clean rooms. mold loves to hide in small crevices. it happens to the best of us, and no one should be ashamed of it. that kind of pride will be a persons downfall and should be avoided at all costs. lets be real. its ok, it doesn't make you a bad person, or a bad grower. usually, the opposite is true, and it will make you a better one if ur observant enough about the situation.
aint that the truth well said seems like whenever I get a contam it's always an epic 20 jar fail lol but eh it is what it is
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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lol yea, i hear that man. truth be told, even g2g can fail us like that. agar plates are key. assuming technique is on point, the OG plate will always tell the whole story in those cases.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Man I went for like a year and a half not able to grow a fucking thing past first flush, and only 50% of what I tried even made it that far.
But I'll be goddamned if I'm not an expert on contamination now though. Keep at it and learn as you go.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Hidden contams [Re: maddchef]
#22122009 - 08/21/15 11:29 AM (8 years, 5 months ago) |
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and a quick update. This contam.. I've been keeping it around out of laziness really. Too lazy to throw it out 
Quote:
This is a random white contamination I got I wanted to share, showing how similar some white molds can be. This right here is why you NEED to be thorough. The contamination is circled.

This is another one, except the circled in part is healthy mycelium:

Since these were transfers that went wrong, I know for a fact everything outside of the spots I transfered to are contaminations (also I've been keeping a close eye on them since I noticed the white growths).
Well here they are now! Exactly 3 weeks later!
First one:
 Notice how much the growth has grown out.. 
Second one:
The clean growth is black, but what's interesting is the mycelium colonized through the stuff highlighted in red. HOWEVER. The white growths and shit in the red shows how unorganized it is. This is something you SHOULD NEVER trust. Mycelium colonizing contams doesn't mean it vanquished it. The contamination is still there, and it's still dormant.
It's also interesting to note.. they both smelled extremely mushroomy.. If I was to use my nose, I'd think they were clean. Even though I know they're not. This is why I really don't like advising people to sniff shit (unless your spawning or birthing, then sniffing every jar is recommended ). Your eyes are 10x better at spotting crap during colonization though.
Edited by Mad Season (08/21/15 11:40 AM)
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MysticMoteToter



Registered: 07/26/15
Posts: 2,036
Loc: Who nose.
Last seen: 12 hours, 1 minute
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Quote:
Mad Season said: If you get sleeves of petris, they're pre-sterilized.
I know this is a somewhat old thread but i was wondering if its okay to only use half of the petris in the sleeve. I know they come with some type of gas in it that keeps it sterile but once i open the sleeve is it okay to use half and then reuse them later?
-------------------- Half Homo Hardly Sapient Overview Effect Fuck War, Feed Birds.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 18 hours, 14 minutes
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There's no sterilizing gas. They're sterilized with radiation. You just have to keep them sterile by closing off the sleeve they're before you take them out of the SAB/FH.
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MysticMoteToter



Registered: 07/26/15
Posts: 2,036
Loc: Who nose.
Last seen: 12 hours, 1 minute
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Re: Hidden contams [Re: Kizzle]
#22375480 - 10/13/15 08:12 PM (8 years, 3 months ago) |
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awesome man thank you!
-------------------- Half Homo Hardly Sapient Overview Effect Fuck War, Feed Birds.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Anyone got more pics of hidden/meshed molds?
Ever since I suspected a few plates of having them every piece of mycelium seems suspect now.
This thread is a goldmine by the way, shame it is not full of pics. Hopefully I won't have resurrected an old thread for nothing :P.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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I wish. I haven't seen many with infused molds. Really just the one from madd is about all I've seen.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Too bad. Been noticing some suspect mycelium in some of my cultures. Not knowing for sure is a nuisance, guess I will have to test them out by fruiting one by one.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Make sure they're uniform and organized. It can take many, many transfers sometimes. If they're not organized and uniform, they're not clean in my books. I've never had a culture I couldn't clean up. Even plates covered in bacteria before any cube germination I've saved with just transfers (no antibacterial agents used). I've seen lots tell others it is garbage when it's pure bacteria, but it isn't the case if you enjoy cleaning cultures lol. You get a huge sense of fulfillment when it works
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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That's the problem though, they look organised but strange, like having trouble growing over the agar surface or going from rhizo to wispy after a xfer when the recipe is the same.
I'm stuck changing my decision every 15 mins I spend staring down at them.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Like the one below. Some parts it's organised and beautiful yet all around it is all this strange growth. Strange thing is, the sector I took that transfer from was totally rhizo with no cottony growth at all.

There are others which I took transfers from nice rhizomorphic growth but they have a hard time staying above the surface and growth is all strange.

I tried to take clear pics of the growth under the surface the other day, this is the best I could come up with.

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SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
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Would you say this culture has a slight hidden bacterial issue, Mad? It was grown out with slow pin formation, and results weren't anything to write home about.
-------------------- AMU Q&A
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
SloppyJoseph said: Would you say this culture has a slight hidden bacterial issue, Mad? It was grown out with slow pin formation, and results weren't anything to write home about.

Looks off. Theres a lot of "aerial" growths. I would try to transfer until it is smooth.
Quote:
Supalemonhaze said: Like the one below. Some parts it's organised and beautiful yet all around it is all this strange growth. Strange thing is, the sector I took that transfer from was totally rhizo with no cottony growth at all.

There are others which I took transfers from nice rhizomorphic growth but they have a hard time staying above the surface and growth is all strange.

I tried to take clear pics of the growth under the surface the other day, this is the best I could come up with.
 
So you're concerned because it grows under the surface? Like to the bottom of the dish under the agar? If you use food colouring, you'll see the myc break it down.
The first has many sectors because it still has lots of genetics in it. Keep transferring from the leading edge until you get to it looking like this:

If you're talking about the thin film around it, that is just mycelium.
The last 3 pics, the first 2 need to grow out more before making much of a call on it, but so far they look alright to me, the last one has leading edges perfect for transfers. It can take lots of transfers before they look uniform and organized.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Yeah, the stuff under the surface is what I meant.
I have never really looked at them with this much detail before so that's why I'm not entirely sure about it but it does seem awfully suspicious to me. I have never seen mycelium do this before. I know mycelium does colonize agar completely but never really done so like this before.
Guess I will know for sure once I try and fruit some of these.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 18 hours, 14 minutes
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Quote:
Supalemonhaze said: Like the one below. Some parts it's organised and beautiful yet all around it is all this strange growth. Strange thing is, the sector I took that transfer from was totally rhizo with no cottony growth at all.

There are others which I took transfers from nice rhizomorphic growth but they have a hard time staying above the surface and growth is all strange.

I tried to take clear pics of the growth under the surface the other day, this is the best I could come up with.
 
Sometimes that just happens, especially when transferring to a different agar mix. Keep transferring until you get a monoculture though. It's the best way to help ensure it's pure.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Hidden contams [Re: Kizzle]
#23178328 - 05/01/16 11:17 PM (7 years, 8 months ago) |
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Does mycelium climbing the walls of a colonized plate definitely mean it's bacterial, or could it be because some of the agar liquid is actually on the sides of the plate (or in my case glad mini rounds)?
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