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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Hidden contams 20
#22020260 - 07/30/15 03:05 PM (8 years, 6 months ago) |
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Bacteria
A prime example of hidden bacteria from kizzle:
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Kizzle said:
 Sneaky bacteria
 Not so sneaky bacteria
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Mad Season said: Super sneaky.. where the hell is it kizzle lol. How do you know it has bacteria?
Is it the off center circle taking up almost all of the dish?
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Kizzle said: It's the very light film covering the "uncolonized" area. I don't know how many dishes like that slipped by before I finally noticed it but I started to wonder why the mycelium was suddenly stalling instead of colonizing the whole plate 
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blindingleaf said: bacteria on WKZ culture. both plates started off looking rhizo/normal, but as they aged, several sprouts popped up, and the second plate, u can see the edges of the growth are bubbling up in response to bacteria. these are both from same clone, same day.
 
another one (slight bacteria/hidden something), matazapec, MS, unfruited

compare to a very clean Penis envy culture

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LocN9ne said: Fairly certain these are all bacteria, except the couple obvious molds on that multi plate...

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WeavieWonder said:

White molds
This is a random white contamination I got I wanted to share, showing how similar some white molds can be. This right here is why you NEED to be thorough. The contamination is circled.

This is another one, except the circled in part is healthy mycelium:

Since these were transfers that went wrong, I know for a fact everything outside of the spots I transfered to are contaminations (also I've been keeping a close eye on them since I noticed the white growths).
Exactly 3 weeks later
First one:
 Notice how much the growth has grown out.. 
Second one:
The clean growth is black, but what's interesting is the mycelium colonized through the stuff highlighted in red. HOWEVER. The white growths and shit in the red shows how unorganized it is. This is something you SHOULD NEVER trust. Mycelium colonizing contams doesn't mean it vanquished it. The contamination is still there, and it's still dormant.
It's also interesting to note.. they both smelled extremely mushroomy.. If I was to use my nose, I'd think they were clean. Even though I know they're not. This is why I really don't like advising people to sniff shit (unless your spawning or birthing, then sniffing every jar is recommended ). Your eyes are 10x better at spotting crap during colonization though.
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Kizzle said: Here's one with hidden Aspergillus.

This is a clone of one of the agar pins.

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blindingleaf said: here is a good pic of a hidden contam. its verticillium fungicola and its a white mold. it was a nuisance to me for many months. found out it was riding on the myc, but would not show itself until a week+ after full colonization of plate. top left is vert only top right is vert + cube mycelium after plate consolidated for a week or two bottom plate is healthy cube mycelium after 2 week consolidation.

note the intertwined plate has a 3d fuzz look to it. starts in the middle, and works its way out

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bodhisatta said:

its hard to tell from pictures because of shitty white balance settings but though the cobweb on this plate looks white it was pretty off white. the cube mycelium is in the middle it's the brighter white and it was completely overcame.


cobweb is a bit of a bad word since there's cobweb disease which is a disease of the fruit body crop. and then what shroomery calls cobweb mold which can be many species of molds with a similar morphology. but may have very different spores for instance
Unknowns and mixture between the 2
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maddchef said:

From the back with a light through it

Honestly I'm not sure exactly what it is but it only showed up after about 15 days post transfer and is in every single transfer from the mother plate.
This is likely the reason I was throwing all my stuff away
This is a great example to show how sometimes it can get so intertwined you'd need to be to get it undone.
If you look carefully, the areas that are clean are always organized. It's the main reason I go for rizmorphic organized areas
Here are some examples of organized clean plates:
For this one I would stay clear of the stuff on the bottom one at 6 o'clock.

Turned into:

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blindingleaf said: a very clean Penis envy culture

Some species/varieties don't have rizmorphs. Like panaeolus species, but I still aspire for organization:

It's just crazy how close and hidden they all look, and makes me wonder how many contaminations I've had slip under the radar. The majority of these contams will most likely still have fruits if they don't turn green during spawning or fruiting. You'd just have a crappie pinset/BE. Or in the case of bl, a massive vert infestation on your fruits. Since I've become thorough, I RARELY see any contaminations. This right here is why I never say that I don't need to improve my techniques, or that I'm 100% clean. I'm not, and I'm constantly working on my sterile procedures.
Edited by Mad Season (04/09/17 09:56 AM)
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Syntheticwords
Victus


Registered: 08/13/11
Posts: 375
Last seen: 6 years, 10 months
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I've just begun working with agar myself
I'll be keeping my eye out for these sneaky bastards
Performing my first transfer from a clone agar to another plate
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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this is why even though i love my pasty plates and what not i always finish on petri's, i get a much better picture, nice pics and good post
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Hidden contams [Re: cronicr]
#22020330 - 07/30/15 03:17 PM (8 years, 6 months ago) |
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cronicr said: this is why even though i love my pasty plates and what not i always finish on petri's, i get a much better picture, nice pics and good post
Thanks man, also I'm getting my hands on petris in bulk. You're absolutely right. That is what makes petris superior. I was actually just thinking about this the other day, and this is kind of the icing on the cake, so to speak.
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Syntheticwords
Victus


Registered: 08/13/11
Posts: 375
Last seen: 6 years, 10 months
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I need something to not melt in the pc, to pour in my SAB
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 4 months
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Re: Hidden contams [Re: cronicr]
#22020365 - 07/30/15 03:24 PM (8 years, 6 months ago) |
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Quote:
cronicr said: this is why even though i love my pasty plates and what not i always finish on petri's, i get a much better picture, nice pics and good post
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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jbaby007
Badass



Registered: 02/28/15
Posts: 1,026
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I've learned my lesson the hard way when it comes to hidden contams. The white molds looking so similar seemed OK to me until I ended up with a sneaky contaminated jar and g2ging it. I'm glad you guys post things like this. It really is very helpful. I'll definitely invest in some petris here soon.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Hidden contams [Re: jbaby007] 1
#22021624 - 07/30/15 07:18 PM (8 years, 6 months ago) |
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I still have a culture that has a mold intertwined so tight that you can't tell its there. Grow it out and try to fruit it and you can.
It's fucking spooky when it happens to you, you become paranoid that every piece of mycelium looks suspect.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Hidden contams [Re: maddchef]
#22021702 - 07/30/15 07:35 PM (8 years, 6 months ago) |
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@synthetic, thanks and I hope it helps when you're working with agar. If you get sleeves of petris, they're pre-sterilized. They don't need to get pc'd. You can use glass petris too, but I think disposable petris are just easier, stack nicer, and nicer to look through. Plus they don't need a pc cycle.
@jbaby thanks 
@madd haha honestly, if you look carefully, the areas that are clean are always organized. It's the main reason I go for rizmorphic organized areas.. However as you can see in kizzles first picture, clean and organized looking doesn't mean you're in the clear. So yeah being paranoid and thorough on agar is how you get success 
Also do you have pics of said intertwined contamination? That'd be really cool to see
Edited by Mad Season (07/30/15 07:46 PM)
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 19 hours, 10 minutes
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Here's one with hidden Aspergillus.

This is a clone of one of the agar pins.

Quote:
Plus they don't need a pc cycle.
Glass petris should really be oven sterilized anyway.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Hidden contams [Re: Kizzle]
#22023160 - 07/31/15 05:59 AM (8 years, 6 months ago) |
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Aspergillus! I've had a couple transfers that looked kinda fluffy/tomentose and pure white, that turned out to be aspergillus. Glad you got some pics of it kizzle
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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How do I bump? god I love contams. Well it's a love hate relationship. I love it when you work your way around or through them. I'm just rambling. Cheddar cheese anyone?
In all seriousness, I hope this helps someone who's having major contamination issues realize that maybe they need to take a step back, read my improve your sterile techniques link in my signature, and try it all over again. I've had to go back to spores on agar before. Won't be the last time either
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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purplecap
candyflipper


Registered: 06/10/15
Posts: 150
Last seen: 1 year, 6 months
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Re: Hidden contams [Re: cronicr]
#22045824 - 08/05/15 05:52 AM (8 years, 6 months ago) |
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nice thread. I was recently made aware that I have been probably transfering mold from one plate to the other just because I assumed that the myc of that variety was puffier. How many times can you transfer from plate to plate to keep stuff going ? I have no slants for cultures but I have a lot of petris
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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That was an interesting read
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
purplecap said: nice thread. I was recently made aware that I have been probably transfering mold from one plate to the other just because I assumed that the myc of that variety was puffier. How many times can you transfer from plate to plate to keep stuff going ? I have no slants for cultures but I have a lot of petris 
You can transfer weekly for well over 6 months. They say to g2g only 3 times max due to the amount you're expanding the mycelium, and rough housing it due to shaking it and breaking it up. It'll bring on senescence way quicker. With agar you can go well over 20 times. If you pour your dishes a bit thick, you can let it colonize and store it in the fridge in petris for a few months.
Generally I transfer once or twice to ensure it's clean. Also every variety can be fuzzy/tomentose. My ape and aa+ are extremely fuzzy looking. However there's contaminations that are fuzzy looking too . That's why rizmorphic and organized is the best thing to go for. Especially when starting out.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
That's why rizmorphic and organized is the best thing to go for. Especially when starting out.
QFT. not saying tomentose can't perform, but as far as safety goes, i also go for rhizomorphic.
awesome thread man. half of me hopes to have some more con tams to add pictures, the other half does not :P
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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about the contams. Too true! I wanna get some more bacteria stuff. I've never really had a hidden bacteria contam problem. Not that I really should be complaining . I actually love getting contams. It's some good experience.
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Quote:
Mad Season said:
about the contams. Too true! I wanna get some more bacteria stuff. I've never really had a hidden bacteria contam problem. Not that I really should be complaining . I actually love getting contams. It's some good experience.
Contams make me feel like I have poison growing in my house.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Hidden contams [Re: Dr.Satan]
#22046441 - 08/05/15 09:47 AM (8 years, 6 months ago) |
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