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InvisibleMad Season
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Hidden contams * 20
    #22020260 - 07/30/15 03:05 PM (8 years, 6 months ago)

Bacteria

A prime example of hidden bacteria from kizzle:

Quote:

Kizzle said:

Sneaky bacteria


Not so sneaky bacteria



Quote:

Mad Season said:
Super sneaky.. where the hell is it kizzle lol. How do you know it has bacteria?

Is it the off center circle taking up almost all of the dish?



Quote:

Kizzle said:
It's the very light film covering the "uncolonized" area. I don't know how many dishes like that slipped by before I finally noticed it but I started to wonder why the mycelium was suddenly stalling instead of colonizing the whole plate :lol:



Quote:

blindingleaf said:
bacteria on WKZ culture.  both plates started off looking rhizo/normal, but as they aged, several sprouts popped up, and the second plate, u can see the edges of the growth are bubbling up in response to bacteria.  these are both from same clone, same day.



another one (slight bacteria/hidden something), matazapec, MS, unfruited


compare to a very clean Penis envy culture




Quote:

LocN9ne said:
Fairly certain these are all bacteria, except the couple obvious molds on that multi plate...




Quote:

WeavieWonder said:
   




White molds


This is a random white contamination I got I wanted to share, showing how similar some white molds can be. This right here is why you NEED to be thorough. The contamination is circled.



This is another one, except the circled in part is healthy mycelium:



Since these were transfers that went wrong, I know for a fact everything outside of the spots I transfered to are contaminations (also I've been keeping a close eye on them since I noticed the white growths).

Exactly 3 weeks later

First one:

Notice how much the growth has grown out.. :thumbdown:

Second one:

The clean growth is black, but what's interesting is the mycelium colonized through the stuff highlighted in red. HOWEVER. The white growths and shit in the red shows how unorganized it is. This is something you SHOULD NEVER trust. Mycelium colonizing contams doesn't mean it vanquished it. The contamination is still there, and it's still dormant.

It's also interesting to note.. they both smelled extremely mushroomy.. If I was to use my nose, I'd think they were clean. Even though I know they're not. This is why I really don't like advising people to sniff shit (unless your spawning or birthing, then sniffing every jar is recommended :thumbup:). Your eyes are 10x better at spotting crap during colonization though.

Quote:

Kizzle said:
Here's one with hidden Aspergillus.


This is a clone of one of the agar pins.




Quote:

blindingleaf said:
here is a good pic of a hidden contam.
its verticillium fungicola and its a white mold.
it was a nuisance to me for many months.
found out it was riding on the myc, but would not show itself until a week+ after full colonization of plate.
top left is vert only
top right is vert + cube mycelium after plate consolidated for a week or two
bottom plate is healthy cube mycelium after 2 week consolidation.


note the intertwined plate has a 3d fuzz look to it.  starts in the middle, and works its way out




Quote:

bodhisatta said:


its hard to tell from pictures because of shitty white balance settings but though the cobweb on this plate looks white it was pretty off white. the cube mycelium is in the middle it's the brighter white and it was completely overcame.




cobweb is a bit of a bad word since there's cobweb disease which is a disease of the fruit body crop. and then what shroomery calls cobweb mold which can be many species of molds with a similar morphology. but may have very different spores for instance




Unknowns and mixture between the 2

Quote:

maddchef said:


From the back with a light through it



Honestly I'm not sure exactly what it is but it only showed up after about 15 days post transfer and is in every single transfer from the mother plate.

This is likely the reason I was throwing all my stuff away



This is a great example to show how sometimes it can get so intertwined you'd need to be :wizard: to get it undone.



If you look carefully, the areas that are clean are always organized. It's the main reason I go for rizmorphic organized areas


Here are some examples of organized clean plates:

For this one I would stay clear of the stuff on the bottom one at 6 o'clock.


Turned into:



Quote:

blindingleaf said:
a very clean Penis envy culture






Some species/varieties don't have rizmorphs. Like panaeolus species, but I still aspire for organization:



It's just crazy how close and hidden they all look, and makes me wonder how many contaminations I've had slip under the radar. The majority of these contams will most likely still have fruits if they don't turn green during spawning or fruiting. You'd just have a crappie pinset/BE. Or in the case of bl, a massive vert infestation on your fruits. Since I've become thorough, I RARELY see any contaminations. This right here is why I never say that I don't need to improve my techniques, or that I'm 100% clean. I'm not, and I'm constantly working on my sterile procedures.


--------------------
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Edited by Mad Season (04/09/17 09:56 AM)


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OfflineSyntheticwords
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Re: Hidden contams [Re: Mad Season]
    #22020293 - 07/30/15 03:10 PM (8 years, 6 months ago)

I've just begun working with agar myself

I'll be keeping my eye out for these sneaky bastards

Performing my first transfer from a clone agar to another plate


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OfflinecronicrFacebook
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Re: Hidden contams [Re: Syntheticwords] * 1
    #22020298 - 07/30/15 03:12 PM (8 years, 6 months ago)

this is why even though i love my pasty plates and what not i always finish on petri's, i get a much better picture, nice pics and good post


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InvisibleMad Season
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Re: Hidden contams [Re: cronicr]
    #22020330 - 07/30/15 03:17 PM (8 years, 6 months ago)

Quote:

cronicr said:
this is why even though i love my pasty plates and what not i always finish on petri's, i get a much better picture, nice pics and good post




Thanks man, also I'm getting my hands on petris in bulk. You're absolutely right. That is what makes petris superior. I was actually just thinking about this the other day, and this is kind of the icing on the cake, so to speak.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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OfflineSyntheticwords
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Re: Hidden contams [Re: Mad Season]
    #22020351 - 07/30/15 03:21 PM (8 years, 6 months ago)

I need something to not melt in the pc, to pour in my SAB


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OfflinetaGyo
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Re: Hidden contams [Re: cronicr]
    #22020365 - 07/30/15 03:24 PM (8 years, 6 months ago)

Quote:

cronicr said:
this is why even though i love my pasty plates and what not i always finish on petri's, i get a much better picture, nice pics and good post



:super:


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Invisiblejbaby007
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Re: Hidden contams [Re: Mad Season]
    #22021420 - 07/30/15 06:40 PM (8 years, 6 months ago)

I've learned my lesson the hard way when it comes to hidden contams. The white molds looking so similar seemed OK to me until I ended up with a sneaky contaminated jar and g2ging it. I'm glad you guys post things like this. It really is very helpful. I'll definitely invest in some petris here soon.


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Invisiblemaddchef
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Re: Hidden contams [Re: jbaby007] * 1
    #22021624 - 07/30/15 07:18 PM (8 years, 6 months ago)

I still have a culture that has a mold intertwined so tight that you can't tell its there. Grow it out and try to fruit it and you can.

It's fucking spooky when it happens to you, you become paranoid that every piece of mycelium looks suspect.


--------------------
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InvisibleMad Season
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Re: Hidden contams [Re: maddchef]
    #22021702 - 07/30/15 07:35 PM (8 years, 6 months ago)

@synthetic, thanks :smile: and I hope it helps when you're working with agar. If you get sleeves of petris, they're pre-sterilized. They don't need to get pc'd. You can use glass petris too, but I think disposable petris are just easier, stack nicer, and nicer to look through. Plus they don't need a pc cycle.

@jbaby thanks :smile:

@madd haha honestly, if you look carefully, the areas that are clean are always organized. It's the main reason I go for rizmorphic organized areas.. However as you can see in kizzles first picture, clean and organized looking doesn't mean you're in the clear. So yeah being paranoid and thorough on agar is how you get success :thumbup:

Also do you have pics of said intertwined contamination? That'd be really cool to see :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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Edited by Mad Season (07/30/15 07:46 PM)


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OfflineKizzle
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Re: Hidden contams [Re: Mad Season]
    #22022812 - 07/31/15 01:10 AM (8 years, 6 months ago)

Here's one with hidden Aspergillus.


This is a clone of one of the agar pins.


Quote:

Plus they don't need a pc cycle.



Glass petris should really be oven sterilized anyway.


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InvisibleMad Season
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Re: Hidden contams [Re: Kizzle]
    #22023160 - 07/31/15 05:59 AM (8 years, 6 months ago)

Aspergillus! I've had a couple transfers that looked kinda fluffy/tomentose and pure white, that turned out to be aspergillus. Glad you got some pics of it kizzle :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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InvisibleMad Season
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Re: Hidden contams [Re: Mad Season]
    #22045438 - 08/05/15 12:52 AM (8 years, 6 months ago)

How do I bump? :lol: god I love contams. Well it's a love hate relationship. I love it when you work your way around or through them. I'm just rambling. Cheddar cheese anyone?

In all seriousness, I hope this helps someone who's having major contamination issues realize that maybe they need to take a step back, read my improve your sterile techniques link in my signature, and try it all over again. I've had to go back to spores on agar before. Won't be the last time either


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflinecronicrFacebook
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Re: Hidden contams [Re: Mad Season]
    #22045446 - 08/05/15 12:52 AM (8 years, 6 months ago)

:bump:


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Offlinepurplecap
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Re: Hidden contams [Re: cronicr]
    #22045824 - 08/05/15 05:52 AM (8 years, 6 months ago)

nice thread. I was recently made aware that I have been probably transfering mold from one plate to the other just because I assumed that the myc of that variety was puffier. How many times can you transfer from plate to plate to keep stuff going ? I have no slants for cultures but I have a lot of petris :smile:


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InvisibleDr.Satan
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Re: Hidden contams [Re: purplecap]
    #22045895 - 08/05/15 06:33 AM (8 years, 6 months ago)

That was an interesting read :fuckinawesome:


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InvisibleMad Season
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Re: Hidden contams [Re: purplecap]
    #22046070 - 08/05/15 08:04 AM (8 years, 6 months ago)

Quote:

purplecap said:
nice thread. I was recently made aware that I have been probably transfering mold from one plate to the other just because I assumed that the myc of that variety was puffier. How many times can you transfer from plate to plate to keep stuff going ? I have no slants for cultures but I have a lot of petris :smile:



You can transfer weekly for well over 6 months. They say to g2g only 3 times max due to the amount you're expanding the mycelium, and rough housing it due to shaking it and breaking it up. It'll bring on senescence way quicker. With agar you can go well over 20 times. If you pour your dishes a bit thick, you can let it colonize and store it in the fridge in petris for a few months.

Generally I transfer once or twice to ensure it's clean. Also every variety can be fuzzy/tomentose. My ape and aa+ are extremely fuzzy looking. However there's contaminations that are fuzzy looking too :thumbdown:. That's why rizmorphic and organized is the best thing to go for. Especially when starting out.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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Invisibleblindingleaf
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Re: Hidden contams [Re: Mad Season]
    #22046133 - 08/05/15 08:22 AM (8 years, 6 months ago)

Quote:

That's why rizmorphic and organized is the best thing to go for. Especially when starting out.




QFT.
not saying tomentose can't perform, but as far as safety goes, i also go for rhizomorphic.

awesome thread man.  half of me hopes to have some more con tams to add pictures, the other half does not :P


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InvisibleMad Season
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Re: Hidden contams [Re: blindingleaf]
    #22046375 - 08/05/15 09:31 AM (8 years, 6 months ago)

:ilold: about the contams. Too true! I wanna get some more bacteria stuff. I've never really had a hidden bacteria contam problem. Not that I really should be complaining :lol:. I actually love getting contams. It's some good experience.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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InvisibleDr.Satan
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Re: Hidden contams [Re: Mad Season]
    #22046393 - 08/05/15 09:35 AM (8 years, 6 months ago)

Quote:

Mad Season said:
:ilold: about the contams. Too true! I wanna get some more bacteria stuff. I've never really had a hidden bacteria contam problem. Not that I really should be complaining :lol:. I actually love getting contams. It's some good experience.



Contams make me feel like I have poison growing in my house. :feelsbadman:


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InvisibleMad Season
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Re: Hidden contams [Re: Dr.Satan]
    #22046441 - 08/05/15 09:47 AM (8 years, 6 months ago)

:rofl: until you find out the different uses each of those contams have. Like the black mold aspergillus Niger. It can actually be eaten and used for enzymes. Penicillium is used to make penicillin and cheese. Certain yeasts can make different things with just sugar water. Like alcohol. I've heard of people isolating yeast in the air and using it to brew some insane wines.

There's lots of other things out there just waiting to be discovered through the processing of molds. Mycology is such a young science. If you get a microscope you can even start looking at the contams under the lens. Which I NEED to start doing. I can't believe I don't have a microscope yet :sad:.. here's a video showing their beauty:

But yeah for this particular hobby, contams are the bane of our existence :lol:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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