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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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30 days later, the jars....
#22017905 - 07/29/15 11:44 PM (8 years, 5 months ago) |
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did nothing. ONE of them was able to fully colonize, 2 others are trying, but overall the Agar piece covered in myc just simply turned into a small lump... And they've been lumps for quite a while. Given, some of the jars are 4 weeks old, some of them are 3 and some are 2, i expected i'd see more of them act l;ike the first jar. The first group i did was five or six jars, and only two (out of 15) are anywhere close.... Could it be too dry?
Could i put some of this jar into the other jars via transfer in my SAB with a sterilized spoon? after all, each agar piece probably had about 1/3 CC of columbian rust growing on it, I see no reason I couldnt do grain to grain like that?
I keep it in a 66qt storage tub, top off. in a room that's either AC at 69/70, or hot as balls at around 80. and the light varies on what comes in my window, but there's a curtain over it so it isnt too bright.... doesnt seem like too much of an issue. Reccomendations here? i could be missing out on several several ounces here
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new2doinstuff
Traveller


Registered: 06/15/14
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Last seen: 5 years, 9 months
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also- how much shaking is too much, and what is specifically a recovery? I'm about to do different jars in a few days from agar again if i have to dump these and restart so I shouldn't even really care. I'm probably going to do 2 shakes next time. first growth, and 50%.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
new2doinstuff said: The first group i did was five or six jars, and only two (out of 15) are anywhere close.... Could it be too dry?
I would say so, last time i prepped some rye jars i put a piece of colonized brf paste in the jars and no matter how much I distributed it within the grains, it just clumped up and didn't spread. What are your PC'ing times and how long do you dry your grains before loading them? I always air dry my grains on a window screen for 20 minutes or until the outside of the grain doesn't feel moist. Put some grains on a piece of toilet paper, it shouldn't leave behind a wet spot, that's how you know it has the perfect moisture content. Then load them up! 
Quote:
new2doinstuff said: Could i put some of this jar into the other jars via transfer in my SAB with a sterilized spoon? after all, each agar piece probably had about 1/3 CC of columbian rust growing on it, I see no reason I couldnt do grain to grain like that?
I would go for it, seems like the best option available at this point.
Quote:
new2doinstuff said: I keep it in a 66qt storage tub, top off. in a room that's either AC at 69/70, or hot as balls at around 80. and the light varies on what comes in my window, but there's a curtain over it so it isnt too bright.... doesnt seem like too much of an issue. Reccomendations here? i could be missing out on several several ounces here
Just keep the jars out of direct sunlight and you're good to go!
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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can i PC some water, and put it through the top? like just a few drops or a small squirt (giggity) through the top and shake???
my PC times were 90mins@15psi... this is wbs, not rye.
Also, i left the wbs out for a while on my table after simmering. (will not do next time) i then put it on a paper towel, rolled it back and forth (like a u shape holding the paper towels in my hand) and rolled it back and forth into the jar... it left behind some wet, but it mostly dried and i used a new paper towel for every jar (after they sat on paper towels for a few hours. had to go buy more jars and run an errand) I really think they might need moisture. There's vermiculite in there too, so even if there IS too much moisture, it isnt too much of a problem. i read somewhere if i was worried ab moisture (i was) i could add some verm (i did.)
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
new2doinstuff said: can i PC some water, and put it through the top? like just a few drops or a small squirt (giggity) through the top and shake???
my PC times were 90mins@15psi... this is wbs, not rye.
Also, i left the wbs out for a while on my table after simmering. (will not do next time) i then put it on a paper towel, rolled it back and forth (like a u shape holding the paper towels in my hand) and rolled it back and forth into the jar... it left behind some wet, but it mostly dried and i used a new paper towel for every jar (after they sat on paper towels for a few hours. had to go buy more jars and run an errand) I really think they might need moisture. There's vermiculite in there too, so even if there IS too much moisture, it isnt too much of a problem. i read somewhere if i was worried ab moisture (i was) i could add some verm (i did.)
The verm is what made the jars too dry. The first jars I ever PC'd I added verm to them(I was following an outdated tek), and they made the grains stick to each other more as well as drying out the grains too fast(I added 1/4 cup). Luckily, I was able to get spores to germinate on them. In your case, I would just do the G2G transfer with a fully colonized jar as you mentioned before.
For next time, add some gypsum as you soak the WBS, then let them air-dry for 20 minutes, PC them at the same times, and the GE hole on your lid will take care of the excess moisture. Shaking the grain jar throughout the day will also speed up the process.
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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Hmmm.... if i do a g2g transfer wont i still run into problems with moisture? Putting mycel into a dry jar wont dry out what i put in?
I think i did an outdated tek too, why cant the forum delete all outdated things or edit the tek to say outdated?
anyways, i did about 10quart jars 3/4 full of wbs [ended up being about 16 jars full] how much gypsum should i do with them rather than putting vem in the jars
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Prep new grains and G2G.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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new2doinstuff
Traveller


Registered: 06/15/14
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Last seen: 5 years, 9 months
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Re: 30 days later, the jars.... [Re: taGyo]
#22020327 - 07/30/15 03:17 PM (8 years, 5 months ago) |
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Perfect. Thanks everyone!
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
new2doinstuff said: how much gypsum should i do with them rather than putting vem in the jars
I always add about half a cup to the soak water then stir it up real good. Keep checking on the bucket as their soaking and shake it up a bit more. It always reduces the clumping issues after the PC cycle.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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IMO proper prep > gypsum to prevent clumping.
I save my gypsum for my bulk substrate.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: 30 days later, the jars.... [Re: taGyo]
#22020377 - 07/30/15 03:25 PM (8 years, 5 months ago) |
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Quote:
taGyo said: IMO proper prep > gypsum to prevent clumping.
I always stock up on the gypsum, it doesn't hurt to add it tho. I like to think of it as a backup, in case I fuck up the drying process.
Edited by Psilosoulful (07/30/15 03:26 PM)
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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What's your bulk grain?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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shroomboomer
Stranger



Registered: 05/07/15
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Last seen: 8 years, 4 months
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Re: 30 days later, the jars.... [Re: taGyo]
#22020498 - 07/30/15 03:50 PM (8 years, 5 months ago) |
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Are you getting gas exchange?
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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wbs. and yes i have gas exchange
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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How are the jars lookin now, any pics?
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new2doinstuff
Traveller


Registered: 06/15/14
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Last seen: 5 years, 9 months
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they look not at all right after day 30. im pretty descriptive so ill try my best.
They darkened in the PC, but didnt burn. Innocc'd 7/3, now, there's only one jar (and it looks proper. no colors, no trich) one other looks a little powdered over with myc that seemed to stop growing. most of the jars have some clumps of myc, but they never went any farther than that. I plan on redoing WBS jars, and doing them grain to grain from the one that worked flawlessly and see if i can get 20-25 jars full colonized. If this gets messed up, i have b+/creeper print on agar right now i can do up later
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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If your mycelium is clumping up, then the moisture of the grains is probably too dry. Make sure you do a 18-24 hour soak(with gypsum) followed by a good simmer. I leave it for 10 minutes or until the grains look plump. The most important part is to dry them till the outside is air dry, do the toilet paper test, if it leaves a wet spot after 30 seconds let it keep drying. Then load them up.
IME, rye berries are much harder to get properly hydrated. I've tried all kinds of methods but they always come out on the dry side. WBS is much easier to prep, not to mention more inoculation points for mycelium to spread. WBS ftw!!
Edited by Psilosoulful (07/31/15 08:09 PM)
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new2doinstuff
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Registered: 06/15/14
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Last seen: 5 years, 9 months
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can i add just a little bit of distilled water (I'll probably PC it in a jar anyways)??
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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You're gonna add water to dry WBS and then PC?
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new2doinstuff
Traveller


Registered: 06/15/14
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Last seen: 5 years, 9 months
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no, in the jars already messed up. I would like to add the water to an empty jar, PC it, and add it to the current dry jars, maybe a tablespoon of water per, and shake
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