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OfflineLibertyPryme
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Registered: 06/04/15
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First time agar-pour questions ?
    #22015875 - 07/29/15 04:23 PM (8 years, 6 months ago)

Hello friends, I have a few general questions about my upcoming agar preparation that I will be attempting in the next day or so. This is my first attempt at agar so I feel i need some more info about some of the specific procedures. I will be using the basic MEA type agar mix that will be poured into my petris in my SAB.

First off, after pressure cooking the agar mixture, how long should I keep the actual bottle at a hot temperature ? And what exact temperature should the bottle/mixture be at when I pour it into my plates ? I understand the mixture needs to be at a higher temperature for pouring because it will solidify when cooling.

Also, once each petri has been poured with agar, how long would I expect to wait before I begin to inoculate ? just until the plate is at room temperature ? or until it has begun to visibly solidify ? Should I let the plates consolidate overnight and then inoculate the next day ?

many thanks for any input/tips/tricks


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OfflineMorel Guy
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Re: First time agar-pour questions ? [Re: LibertyPryme]
    #22015957 - 07/29/15 04:40 PM (8 years, 6 months ago)

Cool enough to handle the agar.  Some people store sealed plates in the fridge, to see if they are contaminated.


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"in sterquiliniis invenitur in stercore invenitur"

In filth it will be found in dung it will be found


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OfflineSmelltheflowers
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Re: First time agar-pour questions ? [Re: LibertyPryme]
    #27272118 - 03/27/21 04:47 PM (2 years, 9 months ago)

I would like to know the same thing. How long do I wait to inoculate after pouring agar? Can I put the bags they came in or ziplock bags over them until they are ready to inoculate? Rather than parafilming each dish?


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InvisibleSmartattack
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Re: First time agar-pour questions ? [Re: Smelltheflowers]
    #27272203 - 03/27/21 05:53 PM (2 years, 9 months ago)

Yes you can keep the sleeves clean and carefully recover them all rather than wrapping. I personally like to vet my plates for a week before use, but sometimes I just roll dice because my failure rate is well below low enough to worry about.


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OfflineBrian Jones
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Re: First time agar-pour questions ? [Re: Smartattack]
    #27272222 - 03/27/21 06:20 PM (2 years, 9 months ago)

Using bodhi's video I pour at 117 degrees which gives you enough time to do a sleeve. I've never tried inoculating till the next day.


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"The Rolling Stones will break up over Brian Jones' dead body"    John Lennon

I don't want no commies in my car. No Christians either.

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InvisibleSmartattack
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Re: First time agar-pour questions ? [Re: Brian Jones]
    #27272258 - 03/27/21 06:58 PM (2 years, 9 months ago)

Funny thing. I just plopped a transfer onto some not yet even solidified plates the other night. The myc totally shot out from the transfer crazy fast. It's normal now but it seemed to like the warm bath of goo. 🤷


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: First time agar-pour questions ? [Re: Smartattack]
    #27272271 - 03/27/21 07:09 PM (2 years, 9 months ago)

I wonder if it was frantically searching for a cooler spot.

Have to get another candy thermometer. Normally i saran wrap one to the bottle and wait till 120° to move it into the sab.


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