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Ic4rus
Stranger

Registered: 02/10/15
Posts: 26
Last seen: 8 years, 3 months
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Needles aren't attached to my syringes?
#22015409 - 07/29/15 02:49 PM (8 years, 6 months ago) |
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Hey there guys, I just received my syringes in the mail, and I noticed the needles werent attached to my syringes, but they provided some. Do I need to follow sterile technique to put on the needles?
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SteveRogers
gandy dancer



Registered: 10/24/06
Posts: 3,450
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Re: Needles aren't attached to my syringes? [Re: Ic4rus]
#22015449 - 07/29/15 02:58 PM (8 years, 6 months ago) |
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You can do it in open air, however best practice would be to attach them in a SAB while wearing clean gloves. Sounds like you are probably not using agar. If that is the case then you don't want to contaminate that MS syringe more than it already is.
The needles are sterile out of the blister pack. Just don't touch the opening.
-------------------- "General, I am loyal to nothing......except The Dream"
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Needles aren't attached to my syringes? [Re: SteveRogers]
#22015462 - 07/29/15 02:59 PM (8 years, 6 months ago) |
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Attach the needles, then flame sterilize, don't wipe with iso after flame sterilization.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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SteveRogers
gandy dancer



Registered: 10/24/06
Posts: 3,450
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Re: Needles aren't attached to my syringes? [Re: taGyo]
#22015477 - 07/29/15 03:03 PM (8 years, 6 months ago) |
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good point.
wiping with ISO after flame sterilization is like taking a shower and then drying yourself off with a turd.
-------------------- "General, I am loyal to nothing......except The Dream"
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Ic4rus
Stranger

Registered: 02/10/15
Posts: 26
Last seen: 8 years, 3 months
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Re: Needles aren't attached to my syringes? [Re: SteveRogers]
#22015478 - 07/29/15 03:03 PM (8 years, 6 months ago) |
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Thank you, much appreciated!
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Needles aren't attached to my syringes? [Re: Ic4rus]
#22015483 - 07/29/15 03:04 PM (8 years, 6 months ago) |
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Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation. Don't wipe your needle with alcohol after flame sterilization:
Quote:
swatt_haze said: wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.
Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.
Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar. Grains is 1CC as well.
After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.
If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Re: Needles aren't attached to my syringes? [Re: SteveRogers]
#22015486 - 07/29/15 03:05 PM (8 years, 6 months ago) |
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Quote:
SteveRogers said: good point.
wiping with ISO after flame sterilization is like taking a shower and then drying yourself off with a turd.
I honestly didn't know that though so thanks.
--------------------

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