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Offlinekushroom
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First try at agar
    #22014345 - 07/29/15 10:45 AM (8 years, 6 months ago)

Hey guys i just had my first try at agar resently. Before i get into the post to far imma just apologize before hand if my thoughts are scattered i just doomed a fat bowl with to much kief on top haha :bonghit: Below i posted pictures of three different cubes im trying to work that i recieved as prints thru trade. Im not quite sure what to look for here, i looked at the pictures on the what it should look like thread nd throughout the forum, but mine looks nothing like there growth. I feel like i might have not mixed it well enough, when i look at the bottom of the jar at a horizontel view of a side view of the agar it seems like something settled a little to the bottom but i dont think it would be going if i did it completely wrong. But here is the pictures, also what can i use for a simple innoc loop? Thanks a bunch as always -kushroom



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Edited by kushroom (07/29/15 10:46 AM)


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OfflineTravelAgency
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Re: First try at agar [Re: kushroom]
    #22014363 - 07/29/15 10:49 AM (8 years, 6 months ago)

Me too! Good luck to you.


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Offlinekiksen
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Re: First try at agar [Re: TravelAgency]
    #22014464 - 07/29/15 11:21 AM (8 years, 6 months ago)

Looking good, good job, i am also trying to do it, but in petri dishes


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OfflinetaGyo
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Re: First try at agar [Re: kiksen]
    #22014595 - 07/29/15 11:47 AM (8 years, 6 months ago)

Transfer away from the 3rd jar immediately.

Second one is fucked.

Third one is either fucked or you dropped way to much spore on it :lol:

I use the tip of a screw driver I can flame sterilize.


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Offlinekushroom
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Re: First try at agar [Re: kiksen]
    #22014785 - 07/29/15 12:21 PM (8 years, 6 months ago)

Quote:

taGyo said:
Transfer away from the 3rd jar immediately.

Second one is fucked.

Third one is either fucked or you dropped way to much spore on it :lol:

I use the tip of a screw driver I can flame sterilize.




Where should i transfer from the third jar at? As well for the second one?

Nd oh yeah i kinda loaded it up with spores lol :shrug:

Awesome ill do that thanks for the tip man!

Quote:

kiksen said:
Looking good, good job, i am also trying to do it, but in petri dishes




Thanks man, i hoping it will do me well. Good luck to you with it!


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OfflinetaGyo
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Re: First try at agar [Re: kushroom]
    #22014803 - 07/29/15 12:24 PM (8 years, 6 months ago)

Second one is trash can tek time.

Third one, right in the middle I see mycelium, I also see bacteria to the right of it so you need to seperate those.

First pic I can't see anything, maybe it's all good :shrug:


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Offlinekushroom
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Re: First try at agar [Re: taGyo]
    #22014859 - 07/29/15 12:33 PM (8 years, 6 months ago)

Quote:

taGyo said:
Second one is trash can tek time.

Third one, right in the middle I see mycelium, I also see bacteria to the right of it so you need to seperate those.

First pic I can't see anything, maybe it's all good :shrug:




Thank you very much man, imma go make up some agar now. For the second jar what do you recommend i do to clean it?

Also how should i do about flame sterilizing in a SAB, last time i just put a rubbing alcohol soaked paper towel in there, should i just clean a lighter in throw it in there?


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All submitted posts are by Someone Who Isn't Me (SWIM) -  and in any event are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.


Edited by kushroom (07/29/15 12:36 PM)


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OfflinetaGyo
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Re: First try at agar [Re: kushroom]
    #22014922 - 07/29/15 12:49 PM (8 years, 6 months ago)

Don't bother cleaning the lighter, flame it outside of the SAB, bring it.

Quote:

taGyo said:
Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation.
Don't wipe your needle with alcohol after flame sterilization:

Quote:

swatt_haze said:
wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.





Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.

Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar.
Grains is 1CC as well.

After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.

If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.




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Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Offlinekushroom
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Re: First try at agar [Re: taGyo]
    #22015077 - 07/29/15 01:30 PM (8 years, 6 months ago)

Quote:

taGyo said:
Don't bother cleaning the lighter, flame it outside of the SAB, bring it.

Quote:

taGyo said:
Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation.
Don't wipe your needle with alcohol after flame sterilization:

Quote:

swatt_haze said:
wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.





Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.

Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar.
Grains is 1CC as well.

After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.

If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.







Ive read that before but i would have to flame the plastic nd all.


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OfflinetaGyo
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Re: First try at agar [Re: kushroom]
    #22015087 - 07/29/15 01:34 PM (8 years, 6 months ago)

What?

No. Just flame it outside the SAB. After flaming a needle a phenomenon called thermophoresis occurs, causing all airborne contams to be repelled from the needle, this it's safe to flame outside and move into the SAB. Just do this smoothly so you don't throw air everywhere putting your hands in.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Offlinekushroom
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Re: First try at agar [Re: taGyo]
    #22021215 - 07/30/15 05:59 PM (8 years, 5 months ago)

Quote:

taGyo said:
What?

No. Just flame it outside the SAB. After flaming a needle a phenomenon called thermophoresis occurs, causing all airborne contams to be repelled from the needle, this it's safe to flame outside and move into the SAB. Just do this smoothly so you don't throw air everywhere putting your hands in.




Wow thank you for the detailed responce. You refer to the needle, how bout a innoc loop, does that occur to all metal instruments we use for agar nd what not? What should i do for multiply agar transfers so i dont have to take my arms in and out. Thanks abunch! -Kushroom


--------------------
:/



All submitted posts are by Someone Who Isn't Me (SWIM) -  and in any event are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.


Edited by kushroom (07/30/15 06:00 PM)


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OfflinetaGyo
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Re: First try at agar [Re: kushroom]
    #22021226 - 07/30/15 06:02 PM (8 years, 5 months ago)

Unfortunately you have to leave the SAB every time you flame. If I'm using a loop I flame it and cool it on the receiving plate and then scrape spores off.

A great way to get around holding a lighter is to buy an alcohol lamp.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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OfflineTravelAgency
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Re: First try at agar [Re: taGyo]
    #22021704 - 07/30/15 07:35 PM (8 years, 5 months ago)

I just poor a little alcohol on the bottom of an upturned coffee mug and light it


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OfflinetaGyo
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Re: First try at agar [Re: TravelAgency]
    #22021845 - 07/30/15 08:13 PM (8 years, 5 months ago)

Dangerous but works.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum


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