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tonpole
Stranger
Registered: 02/12/12
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How do professional mycologists select for sectors on species that do not sector?
#22012881 - 07/29/15 12:45 AM (8 years, 6 months ago) |
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Or to put it another way, is agar sector selection specific to rhizomorphic species? As it pertains to my situation i have plates of a cubensis strain that is abnormally fuzzy and will not sector or produce rhizomorphs under any number of isolations, im lost as to what i should be isolating.
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kmetric



Registered: 08/23/14
Posts: 140
Last seen: 7 years, 10 months
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Re: How do professional mycologists select for sectors on species that do not sector? [Re: tonpole]
#22014536 - 07/29/15 11:37 AM (8 years, 6 months ago) |
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Any pictures?
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36fuckin5
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Registered: 08/11/03
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Loc: Diving into Mystical Territori...
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Re: How do professional mycologists select for sectors on species that do not sector? [Re: kmetric]
#22014554 - 07/29/15 11:40 AM (8 years, 6 months ago) |
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They all sector, rhizo or not.
Cut down on the amount of nutrients in your agar and it should go rhizo.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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tonpole
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Re: How do professional mycologists select for sectors on species that do not sector? [Re: 36fuckin5]
#22014691 - 07/29/15 12:05 PM (8 years, 6 months ago) |
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I use the prescribed amount of PDA to water, 1g:25ml, the same amount that other strains rhizo well with. Of course this strain is odd in that it produces much larger mushrooms and heavier flushes than any other of 6 or so strains ive worked with which have all been very rhizo.
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Toadstool5
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Registered: 01/22/15
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Loc: The Golden State
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Re: How do professional mycologists select for sectors on species that do not sector? [Re: tonpole]
#22015533 - 07/29/15 03:14 PM (8 years, 6 months ago) |
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Quote:
They all sector, rhizo or not.
Cut down on the amount of nutrients in your agar and it should go rhizo.

Maybe it is not sectoring because it is already a monoculture? How much are you allowing the transfers to grow out?
I think pictures would help a lot. Or a jeweler's loop
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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matsc
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Re: How do professional mycologists select for sectors on species that do not sector? [Re: Toadstool5]
#22016150 - 07/29/15 05:25 PM (8 years, 5 months ago) |
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Rhizomorphic growth is a behavior/adaptation that the fungus will undertake when it needs to move from one area to another with some alacrity, most often due to nutrient limitation.
If nutrients are abundant, it will just happily grow exponentially via copious hyphal tip growth (aka Fuzzy, diffuse growth). As it gets hungry and it runs low on nutrients to support all those tips, it will instead focus growth in a "single" direction and form rhizomorphs.
If you want to sector something that isnt growing rhizo's, just cut a section away. If you have a microscope you can cut a single tip and isolate it, or just pick a section that looks good to you. Rhizomorphic sections are usually picked because they look impressive and tend to have a dense amount of growth per unit area.
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tonpole
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Re: How do professional mycologists select for sectors on species that do not sector? [Re: matsc]
#22021393 - 07/30/15 06:34 PM (8 years, 5 months ago) |
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Thanks for the information, i wasnt aware that excessive nutrients caused that, my plates do have excess agar as some tends to settle on the bottom when the plates cool.
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