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dWarriors88


Registered: 07/15/08
Posts: 174
Loc: Q
Last seen: 7 years, 11 months
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jj
#22012738 - 07/28/15 11:43 PM (8 years, 6 months ago) |
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jj
Edited by dWarriors88 (02/24/16 09:23 AM)
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DensePlacebo
Stranger



Registered: 05/14/15
Posts: 186
Loc: canada
Last seen: 6 years, 4 months
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Yup. Trich. Garbage.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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jbaby007
Badass



Registered: 02/28/15
Posts: 1,026
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Yes, it's contam. Throw it out. Hopefully you have other projects going. Make sure to go over your sterile technique and try to figure out what went wrong. That's if you made these yourself.
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dWarriors88


Registered: 07/15/08
Posts: 174
Loc: Q
Last seen: 7 years, 11 months
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hh
Edited by dWarriors88 (02/24/16 09:22 AM)
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MikeBearPig
Not liked.


Registered: 07/15/14
Posts: 2,319
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Quote:
dWarriors88 said: Ya, currently have 18, well 17 jars now. I utilized the oven door method, and burned the syringe in between every jar during inoculation. Alcohol swab to wipe the syringe after the burn. I hope I didn't carry to contamination into another jar. Thanks for the quick responses guys.
I am sorry to inform you that the information you got is bad and all your jars are more than likely contaminated. Never wipe after a flame. Never wipe at all, just flame it red.
How ever, what is the oven door method? Are you trying to create a flow hood using your oven?
I know it seems like the air is rising up and it will keep stuff clean, but the air is flowing in the wrong direction. It will rise and pull contaminated air up with it, it is best to do your first inoculations in a still room or a SAb.
Another one for the myth page.
Edited by MikeBearPig (07/29/15 05:15 AM)
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dWarriors88


Registered: 07/15/08
Posts: 174
Loc: Q
Last seen: 7 years, 11 months
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kk
Edited by dWarriors88 (02/24/16 09:22 AM)
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MikeBearPig
Not liked.


Registered: 07/15/14
Posts: 2,319
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Quote:
dWarriors88 said: Well the majority of the jars look healthy thusfar, what is so detrimintal about wiping with an alcohol swab after innoculating? I assumed the heat from the glowing needle would eviscerate the spores considering they don't like high temps? And I just preheat the oven to 350, open the door, pull a rack out, and set cardboard on the rack for a work area. And inoculate in front of the oven. I turned the AC off in the house so shit wouldn't be floating around in the air.
When you wipe a flame sterilized needle, you just wipe it down with all the contaminates on it. It is 100% good to go flame hot, it is not 100% after you introduce contamination's to it.
Spores are ALWAYS in your house, if the AC was on or not. And the oven method does not do shit but stir more contaminates up and possibly in your holes. Use a still room like a bathroom or a Still air box.
I don't want to argue with you about this. I am not posting anymore on here, you will listen when all your jars turn green.
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dWarriors88


Registered: 07/15/08
Posts: 174
Loc: Q
Last seen: 7 years, 11 months
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kk
Edited by dWarriors88 (02/24/16 09:21 AM)
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DensePlacebo
Stranger



Registered: 05/14/15
Posts: 186
Loc: canada
Last seen: 6 years, 4 months
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Don't worry. That's guys seems like a dick who doesn't know what he's talking about. Lots of people wipe there syringe after, it's true its not the best way but its definitely not like all your jars are fucked. Fucking contam nazis gotta watch out. If your worried about it being too hot just squirt a little spore water out first. And the oven tek works wonders for lots of guys. Your bathroom IME is a bad place to do anything. I has black mold after black mold after black mold in jars inoculated in there. I switched to the oven tek for along while before finally building a GB. And I never got black mold again.
-------------------- Alright then, picture this if you will: 10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51. Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this. Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping... Holy fucking shit!
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Don't wipe a flame sterilized needle. Flame STERILIZES, alcohol SANITIZES, big difference.
Quote:
taGyo said: Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation. Don't wipe your needle with alcohol after flame sterilization:
Quote:
swatt_haze said: wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.
Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.
Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar. Grains is 1CC as well.
After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.
If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Quote:
dWarriors88 said: Who's arguing? I'm appreciative of your advice and will improve upon my methods next time. I appologize if my tone came across you negatively.
don't worry about it. some users have little patience for new growers. just a misunderstanding.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Possible early contam? [Re: Munchauzen]
#22014532 - 07/29/15 11:36 AM (8 years, 6 months ago) |
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Oven door is super bad news, and wiping after flaming is also bad news. The alcohol can only be dirtier than the sterile needle you're wiping it on. There is no other possibility. You squirt out a drop or two of liquid to cool the needle, and then proceed with the inoculation as normal. Using a still air box solves both issues, since you can cool the needle before you put it in the jar, AND you might actually be doing it in an environment without contams floating right above your work.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
taGyo said: Don't wipe a flame sterilized needle. Flame STERILIZES, alcohol SANITIZES, big difference.
Quote:
taGyo said: Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation. Don't wipe your needle with alcohol after flame sterilization:
Quote:
swatt_haze said: wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.
Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.
Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar. Grains is 1CC as well.
After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.
If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.
This.
Quote:
DensePlacebo said: Don't worry. That's guys seems like a dick who doesn't know what he's talking about. Lots of people wipe there syringe after, it's true its not the best way but its definitely not like all your jars are fucked. Fucking contam nazis gotta watch out.
Not this. All those people that do wipe after are noobs. I used to use alcohol for my knife on agar and got every contamination in the book. I finally opted for a blow torch and now I have no contaminations.
Edited by Mad Season (07/29/15 11:42 AM)
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Possible early contam? [Re: Mad Season]
#22014562 - 07/29/15 11:41 AM (8 years, 6 months ago) |
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Spreading bad procedure is bad.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
DensePlacebo said: Don't worry. That's guys seems like a dick who doesn't know what he's talking about. Lots of people wipe there syringe after, it's true its not the best way but its definitely not like all your jars are fucked.
This guy is clearly dense and grows placebos. Of all the things that you'd benefit from listening to, this isn't one of them I'm afraid.
@taGyo- for real, shame shame.
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MikeBearPig
Not liked.


Registered: 07/15/14
Posts: 2,319
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Re: Possible early contam? [Re: Inocuole]
#22014578 - 07/29/15 11:43 AM (8 years, 6 months ago) |
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Quote:
Inocuole said: Oven door is super bad news, and wiping after flaming is also bad news. The alcohol can only be dirtier than the sterile needle you're wiping it on. There is no other possibility. You squirt out a drop or two of liquid to cool the needle, and then proceed with the inoculation as normal. Using a still air box solves both issues, since you can cool the needle before you put it in the jar, AND you might actually be doing it in an environment without contams floating right above your work.
I think there is just a confusion on how Alcohol works. It takes 1-2 min to kill things.. So every second you are waving it around and wiping needles, you are adding new life. It is IMPOSSIBLE for a wipe to ever be clean if it is in your hands due to the 1-2 min buffer depending on the concentration of alcohol.
I think people think of it like a nuclear bomb and everywhere they wipe is just instant mass death and genocide.
I apologize, I misread.. It was really early in the morning and I was very tired and agitated that my sleeping meds did not work 5 hours previous.
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dWarriors88


Registered: 07/15/08
Posts: 174
Loc: Q
Last seen: 7 years, 11 months
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hjj
Edited by dWarriors88 (02/24/16 09:20 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
MikeBearPig said:
Quote:
Inocuole said: Oven door is super bad news, and wiping after flaming is also bad news. The alcohol can only be dirtier than the sterile needle you're wiping it on. There is no other possibility. You squirt out a drop or two of liquid to cool the needle, and then proceed with the inoculation as normal. Using a still air box solves both issues, since you can cool the needle before you put it in the jar, AND you might actually be doing it in an environment without contams floating right above your work.
I think there is just a confusion on how Alcohol works. It takes 1-2 min to kill things.. So every second you are waving it around and wiping needles, you are adding new life. It is IMPOSSIBLE for a wipe to ever be clean if it is in your hands due to the 1-2 min buffer depending on the concentration of alcohol.
I think people think of it like a nuclear bomb and everywhere they wipe is just instant mass death and genocide.
I apologize, I misread.. It was really early in the morning and I was very tired and agitated that my sleeping meds did not work 5 hours previous.
1-2 minutes still isn't going to kill everything, and nobody has that kind of time between each jar. Are you.. condoning this or am I misunderstanding?
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MikeBearPig
Not liked.


Registered: 07/15/14
Posts: 2,319
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Re: Possible early contam? [Re: Inocuole]
#22016052 - 07/29/15 05:01 PM (8 years, 5 months ago) |
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The birthing means you made it and you have nothing to worry about other than getting a nice coat of verm on it.
I dunk mine in the fridge, you can dunk them any way you want. We have not found any reason to believe that fridge is different than room temp. As long as your room temp is not scorching hot of course. Use a pot with a lid and place a plate over them so they are 100% submerged.
To get a nice coating of verm on them, I use the biggest possible bowl I can find, load it with 4x as much verm as I plan to use and just submerge the cake and pack it lightly against the sides. If you do this step right after you rinse, then the verm sticks better in my past experiences.
When you put them in the SGFC, make sure the aluminum foil you cut is the circumference of the cake.Quote:
Inocuole said:
Quote:
MikeBearPig said:
Quote:
Inocuole said: Oven door is super bad news, and wiping after flaming is also bad news. The alcohol can only be dirtier than the sterile needle you're wiping it on. There is no other possibility. You squirt out a drop or two of liquid to cool the needle, and then proceed with the inoculation as normal. Using a still air box solves both issues, since you can cool the needle before you put it in the jar, AND you might actually be doing it in an environment without contams floating right above your work.
I think there is just a confusion on how Alcohol works. It takes 1-2 min to kill things.. So every second you are waving it around and wiping needles, you are adding new life. It is IMPOSSIBLE for a wipe to ever be clean if it is in your hands due to the 1-2 min buffer depending on the concentration of alcohol.
I think people think of it like a nuclear bomb and everywhere they wipe is just instant mass death and genocide.
I apologize, I misread.. It was really early in the morning and I was very tired and agitated that my sleeping meds did not work 5 hours previous.
1-2 minutes still isn't going to kill everything, and nobody has that kind of time between each jar. Are you.. condoning this or am I misunderstanding?
I was trying to explain that alcohol sucks balls
For every second the wipe is exposed to the air, it restarts the timer for it to kill what ever lands..
You are CONSTANTLY fighting a 1-2 min timer and NEVER killing everything because it is all dying at a different pace.
Edited by MikeBearPig (07/29/15 05:03 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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