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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Syringe question
#22010646 - 07/28/15 04:29 PM (8 years, 6 months ago) |
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Is the inside of a bought-spore syringe sterile?
I want to try to make my own, and I have a syringe with like 10cc left of solution..if I squirt that out and flame-sterilize the tip...am I good to go?
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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tryptkaloids
Learner



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i'm confused.. you have spore solution but you want to throw it out to make another syringe?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Psilosoulful

Registered: 09/05/14
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I would boil some water and suck it up and squirt it out into the sink a few times to be sure the inside is completely sterile. I've always followed this tek: http://www.shroomery.org/forums/showflat.php/Number/12098357/fpart/all/vc/1
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tryptkaloids
Learner



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why don't you use the left over solution on more cakes/grains? after you are out of solution boil water and suck it up into the syringe and discard a couple times. this will sterilize your syringe and needle. this is all described in the making a syringe tek posted in the mushroom info section
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Well i was going to if i found out from tou guys that i could without making it non sterile. The thing is i only have that one syringe and really need to make a new one with a print because its from a different batch of very potent PE so i easily would have sacrificed that little spore solution left if i had to in order to make a new syringe with this print..its the only one i have left of this because i forgot to make others so i really want to save these genes
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Quote:
Psilosoulful said: I would boil some water and suck it up and squirt it out into the sink a few times to be sure the inside is completely sterile. I've always followed this tek: http://www.shroomery.org/forums/showflat.php/Number/12098357/fpart/all/vc/1
This is all I do and it's been proven to work so far testing the solution on agar dishes. That's always the best move, make sure you succeeded at sterile procedure by testing the solution on an agar dish.
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Re: Syringe question [Re: taGyo]
#22010716 - 07/28/15 04:47 PM (8 years, 6 months ago) |
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Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what im used to doing
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Quote:
Bill_Oreilly said:
Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what in used to doing 
don't do LC, a MS syringe is far more simple to make plus less room for error. Every TC I've come across says don't fuck with LC it's a waste of time.
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Edited by Dr.Satan (07/28/15 04:51 PM)
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Quote:
Bill_Oreilly said:
Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what in used to doing 
Ahh.
So you have an empty store-bought syringe, correct? I would use that boil method they talked about earlier. I've used it twice and it was good, I do my syringes different now though.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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taGyo
Strainiac/AMU



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Posts: 18,802
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Re: Syringe question [Re: Dr.Satan]
#22010740 - 07/28/15 04:50 PM (8 years, 6 months ago) |
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Quote:
Dr.Satan said:
Quote:
Bill_Oreilly said:
Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what in used to doing 
don't do LC, a MS syringe is far more simple to make.
Simpler then Karo and water?
LOL, how simple are you?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Re: Syringe question [Re: taGyo]
#22010746 - 07/28/15 04:52 PM (8 years, 6 months ago) |
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Quote:
taGyo said:
Quote:
Bill_Oreilly said:
Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what in used to doing 
Ahh.
So you have an empty store-bought syringe, correct? I would use that boil method they talked about earlier. I've used it twice and it was good, I do my syringes different now though.
Its a spore syringe bought from a sponser woth about 20cc left in it
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Re: Syringe question [Re: taGyo]
#22010753 - 07/28/15 04:53 PM (8 years, 6 months ago) |
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Quote:
taGyo said:
Quote:
Dr.Satan said:
Quote:
Bill_Oreilly said:
Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what in used to doing 
don't do LC, a MS syringe is far more simple to make.
Simpler then Karo and water?
LOL, how simple are you?
Look, I've never made an LC syringe because the trusted cultivators on here say it's a waste of time. You are the only person I've ever seen on here saying LC is a good idea. So, not to be rude, but I'm going to continue believing it is probably a waste of time. All you need to do is boil water and have a spore print to make a MS syringe.
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spacechildo
proletarians rise up



Registered: 01/24/13
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most bought syringes have a fuck ton of spores in them, sterilize a small jar with water in it and shoot the 10 or 20 cc into it. now you have a jar full of spore water and an empty syringe so you can do the boiling water to syringe thing.
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taGyo
Strainiac/AMU



Registered: 10/16/14
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That's what I thought it was lol.
If you're looking to extend it I would really do an LC or Agar.
@Dr.Satan Which TC? Lol. I've been around the block, LCs aren't a waste of time with a clean syringe, it's a good way to extend a life cycle.
I'm more of an agar man myself but if that's not an option then I would suggest an LC, .25 cc from that syringe can make a whole viable, clean LC with the right technique.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Re: Syringe question [Re: taGyo]
#22010816 - 07/28/15 05:05 PM (8 years, 6 months ago) |
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Quote:
spacechildo said: most bought syringes have a fuck ton of spores in them, sterilize a small jar with water in it and shoot the 10 or 20 cc into it. now you have a jar full of spore water and an empty syringe so you can do the boiling water to syringe thing. 
This seems like the best option for OP. I mean there's little to no way to fuck that up.
Quote:
taGyo said:
@Dr.Satan Which TC? Lol. I've been around the block, LCs aren't a waste of time with a clean syringe, it's a good way to extend a life cycle.
It's not like I remember the TC's usernames, but either way they say LC has far more room for error sterilization wise then making a MS syringe does. I know LC can work given the right technique, but is it really worth all the trouble in comparison?
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SteveRogers
gandy dancer



Registered: 10/24/06
Posts: 3,450
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Quote:
Bill_Oreilly said: so i really want to save these genes
agar and slants FTW. it will change you life man.
-------------------- "General, I am loyal to nothing......except The Dream"
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taGyo
Strainiac/AMU



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Re: Syringe question [Re: Dr.Satan]
#22010837 - 07/28/15 05:09 PM (8 years, 6 months ago) |
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Okay.
I'm sorry, I shouldn't have snapped and been rude.
Here's the breakdown of LC: It is something that is very easy to mess up which is why most people don't recommend it to new members. Bill has been around the block so I think he can comprehend and understand what it takes to make a clean LC.
As Spacechild said, making an LC from an MS syringe bought from a sponsor is very tricky. My first LC, and Spacechild will confirm this, I made was before I even saw my first mushroom. they were all 100% clean and the tub they were used on had a flush so full I had to break mushrooms to harvest it.
LC is a very viable way to extend a clone, syringe, or Liquid Innoculant. You can actually take a part of the LC and squeeze it into more LC water and extend it AGAIN.
Now here is where the difficulty comes in to play: Without agar work to make sure that syringe is clean you could also be breeding a contam. LC is very tricky in the fact that if you are breeding a contam you will most likely not see it so you have to test it on either a jar or on agar.
With proper testing and control, you'll be fine to do an LC. With 20cc of a syringe, that's A LOT of LC.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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spacechildo
proletarians rise up



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Re: Syringe question [Re: taGyo]
#22010851 - 07/28/15 05:13 PM (8 years, 6 months ago) |
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Quote:
taGyo said: As Spacechild said, making an LC from an MS syringe bought from a sponsor is very tricky.
heh, I didn't say it here but its my stance on it yeah. and especially if its just a random syringe you traded/bought.
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Re: Syringe question [Re: taGyo]
#22010858 - 07/28/15 05:16 PM (8 years, 6 months ago) |
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Quote:
taGyo said: Okay.
I'm sorry, I shouldn't have snapped and been rude.
Here's the breakdown of LC: It is something that is very easy to mess up which is why most people don't recommend it to new members. Bill has been around the block so I think he can comprehend and understand what it takes to make a clean LC.
As Spacechild said, making an LC from an MS syringe bought from a sponsor is very tricky. My first LC, and Spacechild will confirm this, I made was before I even saw my first mushroom. they were all 100% clean and the tub they were used on had a flush so full I had to break mushrooms to harvest it.
LC is a very viable way to extend a clone, syringe, or Liquid Innoculant. You can actually take a part of the LC and squeeze it into more LC water and extend it AGAIN.
Now here is where the difficulty comes in to play: Without agar work to make sure that syringe is clean you could also be breeding a contam. LC is very tricky in the fact that if you are breeding a contam you will most likely not see it so you have to test it on either a jar or on agar.
With proper testing and control, you'll be fine to do an LC. With 20cc of a syringe, that's A LOT of LC.
I just checked out your journal and you're far more experienced with this hobby then I would of guessed. I will consider you a trusted cultivator from here on out
--------------------

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tryptkaloids
Learner



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Re: Syringe question [Re: Dr.Satan]
#22010899 - 07/28/15 05:25 PM (8 years, 6 months ago) |
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yea man, taGyo knows his shit he's helped a ton of people out a lot!
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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LoveNaborFuckHater
That one guy


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Re: Syringe question [Re: Dr.Satan]
#22010905 - 07/28/15 05:26 PM (8 years, 6 months ago) |
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If he wanted to keep the potency of the variety he has shouldn't he clone by LC? I was under the impression that if he were to make a syringe out of a print it would just be a MS because that print has a random set of genetics compared to him getting a clone of a potent specimen
-------------------- "They told me drugs were bad, oh man, oh man, they had me fooled"

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taGyo
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I shouldn't have lost my temper though. With that thread with the guy with the cake I always wait for a more positive ID but contams won't spread through a dry verm layer into another jar. It's impossible with the dry verm layer.
Quote:
spacechildo said:
Quote:
taGyo said: As Spacechild said, making an LC from an MS syringe bought from a sponsor is very tricky.
heh, I didn't say it here but its my stance on it yeah. and especially if its just a random syringe you traded/bought.
Guess I'm a mind reader 
Quote:
LoveNaborFuckHater said: If he wanted to keep the potency of the variety he has shouldn't he clone by LC? I was under the impression that if he were to make a syringe out of a print it would just be a MS because that print has a random set of genetics compared to him getting a clone of a potent specimen
Clone is ideal, from that spore syringe he could even grow bunk PE but there are so many possibilities from a spore.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Machiavelliavore
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There are a bunch of stories about mycologists accidentally growing cubes cause a few spores were around when trying to grow exotics.
It's probably sterile with the exception of the spores that are left in it after you empty it.
No reason to be wreckless with a project that will span months, boil dat water and suck'r up.
--------------------
I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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tryptkaloids
Learner



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Re: Syringe question [Re: taGyo]
#22010949 - 07/28/15 05:34 PM (8 years, 6 months ago) |
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what if the contam got in there through a wet spot in the dry verm layer? could it transfer then?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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taGyo
Strainiac/AMU



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Very, extremely unlikely. Probably less likely then a dry verm layer as the water would saturate the medium and make the spores heavier.
You'd have to open it up in your grow area and push some of the contam into the holes and past the dry verm layer of the second brf jar to contam it through the air. We use dry verm because it's very good at what it does, catching anything microscopic that happens to float down.
That or a gust of wind would have to pick up the spores from the first BRF jar and magically transport them over to the second while moving the second jars dry verm.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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LoveNaborFuckHater
That one guy


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Re: Syringe question [Re: taGyo]
#22011005 - 07/28/15 05:44 PM (8 years, 6 months ago) |
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Quote:
taGyo said: Clone is ideal, from that spore syringe he could even grow bunk PE but there are so many possibilities from a spore.
So why throw out what solution he has in his syringe if the new print will result in such a variety of genetics that they'll likely just end up the same as they are MS and not LC? I'm wondering why take from a print because I used MS and my cubes were so weak an 8th barely did anything. Is him using a print v. MS not essentially the same because the print carries just as much variety as an MS will?
-------------------- "They told me drugs were bad, oh man, oh man, they had me fooled"

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taGyo
Strainiac/AMU



Registered: 10/16/14
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Quote:
LoveNaborFuckHater said:
Quote:
taGyo said: Clone is ideal, from that spore syringe he could even grow bunk PE but there are so many possibilities from a spore.
So why throw out what solution he has in his syringe if the new print will result in such a variety of genetics that they'll likely just end up the same as they are MS and not LC? I'm wondering why take from a print because I used MS and my cubes were so weak an 8th barely did anything. Is him using a print v. MS not essentially the same because the print carries just as much variety as an MS will?
If he's not working with LC he's most likely not working with clones yet.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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LoveNaborFuckHater
That one guy


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Re: Syringe question [Re: taGyo]
#22011026 - 07/28/15 05:48 PM (8 years, 6 months ago) |
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I just feel it would be a waste of time and solution if he wants to get that potency he would probably have to clone
-------------------- "They told me drugs were bad, oh man, oh man, they had me fooled"

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taGyo
Strainiac/AMU



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I agree 
But you have to suggest what people are comfortable with. I work with agar and take clones.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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LoveNaborFuckHater
That one guy


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Re: Syringe question [Re: taGyo]
#22011053 - 07/28/15 05:54 PM (8 years, 6 months ago) |
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I don't want other people to waste a multi-month project if they only have one going on MS. It was a learning experience for me but next time I get around to growing I'm definitely going to try cloning with agar
-------------------- "They told me drugs were bad, oh man, oh man, they had me fooled"

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taGyo
Strainiac/AMU



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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Re: Syringe question [Re: taGyo]
#22011104 - 07/28/15 06:05 PM (8 years, 6 months ago) |
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So I shouldn't do the print in hopes to get a potency pretty close to the PE they grow into?
I thought if I took a print of the PE I have, then the genetics would be MS, but also close to what the potency of the mushroom I printed.
if that's not the case im just going to say fuck the whole printing thing and just use the syringe up and just take a print later just for the reason of not having to buy my own.
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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taGyo
Strainiac/AMU



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Okay here's something I recently learned so I hope I'm explaining it right:
Lets say you take a print from Flush A. In that print you have Flush A genetics, Flush B genetics and then a shit ton of other spores. If you happen to get the same potency from the next PE and you print that eventually, after many, many generations of continuing this, you will create a spore print with a base potency. This is the process of training a strain to do a certain thing, for instance most GT and B+ prolifically fruits.
It's just a shot in the dark as to whether you'll find that same exact potency, more potent or completely bunk which is why clone >.
But yes, in theory, if you keep taking prints from a potent cube you will have a higher chance of creating potent cubes through generation after generation. This is also how you breed for leucistic traits and whatnot. Print the leucistic ones until you get all white shrooms.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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LoveNaborFuckHater
That one guy


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If you still have the fruits you took the print from you could just clone and transfer straight to a jar or use those outdoor fruits in your other thread if they end up being PE (correct me if I'm wrong).
-------------------- "They told me drugs were bad, oh man, oh man, they had me fooled"

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taGyo
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I'd put it on agar first though but you can go straight to grain with success, it'll be a bit slow though.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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tryptkaloids
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I'm assuming that by adding cloning to the process you can cut down the time it takes to do that.. if you take tissue from a really tall fruit then the fruits will come out tall. if you print from a rally potent clone that has been isolated over and over again then there will be a higher percentage of the potent genes in it. atleast this is my basic understanding of biology. please correct me if any of this sounds incorrect
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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LoveNaborFuckHater
That one guy


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Re: Syringe question [Re: taGyo]
#22011252 - 07/28/15 06:27 PM (8 years, 6 months ago) |
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Quote:
taGyo said:

I'd put it on agar first though but you can go straight to grain with success, it'll be a bit slow though.
Would cutting out a bigger piece out the fruit make any difference in speed? Someone also told me the other day that there can be differences depending on where you cut from
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tryptkaloids
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i don't see why not, often the amount of inoculant is pretty strong in determining speed of colonisation. at least with liquids. I think it has to do with inoculation points
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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taGyo
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Quote:
tryptkaloids said: I'm assuming that by adding cloning to the process you can cut down the time it takes to do that.. if you take tissue from a really tall fruit then the fruits will come out tall. if you print from a rally potent clone that has been isolated over and over again then there will be a higher percentage of the potent genes in it. atleast this is my basic understanding of biology. please correct me if any of this sounds incorrect

Takes many generations though.
Quote:
LoveNaborFuckHater said:
Quote:
taGyo said:

I'd put it on agar first though but you can go straight to grain with success, it'll be a bit slow though.
Would cutting out a bigger piece out the fruit make any difference in speed? Someone also told me the other day that there can be differences depending on where you cut from
A slight difference . It will definitely be faster but depending on how big the piece not by much. The mycelium has to re-orient itself to colonization and then "leap-off" the clone tissue to begin colonization. First flush is best for this as subsequent flushes have much less vigorous mycelium.
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LoveNaborFuckHater
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Re: Syringe question [Re: taGyo]
#22011336 - 07/28/15 06:38 PM (8 years, 6 months ago) |
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What if I just throw a whole stem in a grain jar?
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taGyo
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Quote:
tryptkaloids said: i don't see why not, often the amount of inoculant is pretty strong in determining speed of colonisation. at least with liquids. I think it has to do with inoculation points
There is such a thing as to much though but yeah, generally more liquid faster colonization. But if you put 2cc in a cake you won't make the cake colonize faster .
Grains are a lot more resistant to water content then cakes.
Quote:
LoveNaborFuckHater said: What if I just throw a whole stem in a grain jar? 
Better be a clean stem
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tryptkaloids
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contam central..
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Bill_Oreilly
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Could you put a mushroom cap in a jar of water then pc it and suck up the water in a syringe? Lol
Which reminds me...could i get a Pf jar..open it and break it up..put it back into the jar..PC it..then throw a few little pieces in a rye berry quart jar? I know its a dumb question
Edited by Bill_Oreilly (07/28/15 07:00 PM)
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taGyo
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You'd kill the mycelium.
Little pieces of BRF? I'd just PC the BRF inside the grain jar together.
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Bill_Oreilly
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Re: Syringe question [Re: taGyo]
#22011405 - 07/28/15 06:49 PM (8 years, 6 months ago) |
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What about the 1st question to make a syringe?
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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LoveNaborFuckHater
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Re: Syringe question [Re: taGyo]
#22011440 - 07/28/15 06:55 PM (8 years, 6 months ago) |
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Would cutting out from the inside of a fruit help prevent from transferring contams to your agar or grain jar?
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tryptkaloids
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aslong as you dont sterilise the cap
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tryptkaloids
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Quote:
LoveNaborFuckHater said: Would cutting out from the inside of a fruit help prevent from transferring contams to your agar or grain jar?
yes this is common practice
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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taGyo
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Quote:
LoveNaborFuckHater said: Would cutting out from the inside of a fruit help prevent from transferring contams to your agar or grain jar?
Yup, that's standard tek 
Quote:
Bill_Oreilly said: Could you put a mushroom cap in a jar of water then pc it and suck up the water in a syringe? Lol
Which reminds me...could i get a Pf jar..open it and break it up..put it back into the jar..PC it..then throw a few little pieces in a rye berry quart jar? I know its a dumb question
The spores die at 122f which a PC reaches and surpasses so I wouldn't try that. Spores are also naturally dirty because they grow underneath the shroom which is exposed to open-air.
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Bill_Oreilly
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Re: Syringe question [Re: taGyo]
#22011497 - 07/28/15 07:06 PM (8 years, 6 months ago) |
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Ok thanks. Im just trying to find the easiest way to make a syringe..buying them is getting too expensive
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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taGyo
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What are your reserves about agar work?
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Bill_Oreilly
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Re: Syringe question [Re: taGyo]
#22011513 - 07/28/15 07:09 PM (8 years, 6 months ago) |
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Yeah i just don't think im ready for it right now. I believe i still have some more learning to do before i jump into the ol agar
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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LoveNaborFuckHater
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Quote:
Bill_Oreilly said: Ok thanks. Im just trying to find the easiest way to make a syringe..buying them is getting too expensive 
That's what I've been trying to think of, I just got a couple ozs of strong but dried cubes and I'm gonna try to drop some water into the cap and hopefully get some spores. Now I just need to order some empty syringes
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Bill_Oreilly
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I mean i havent even made a spore print yet
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Bill_Oreilly
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I was thinking of using this dudes spore syringe tek
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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LoveNaborFuckHater
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Quote:
Bill_Oreilly said: I mean i havent even made a spore print yet 
That's still a goal of mine
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taGyo
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This is the one I followed:
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taGyo
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Re: Syringe question [Re: taGyo]
#22011560 - 07/28/15 07:21 PM (8 years, 6 months ago) |
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The only thing I would change is don't rub everything with iso, wrap it in foil and PC it.
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Bill_Oreilly
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Re: Syringe question [Re: taGyo]
#22011616 - 07/28/15 07:32 PM (8 years, 6 months ago) |
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I just like the other method because you don't need a flowhead/SAB/GB..but man that method looks easy
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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taGyo
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You don't even have an SAB Bill?
, now you gotta step it up man.
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Bill_Oreilly
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Re: Syringe question [Re: taGyo]
#22011630 - 07/28/15 07:35 PM (8 years, 6 months ago) |
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nope 
I do everything the lazy way...PC jars, inoculate them(with sponsers syringes), crumble cakes and spawn to bulk, then throw the trays in humidity domes.
but I do have a PC...im fucking lazy but im not stupid!
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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taGyo
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Get to an SAB bro, then the world is your oyster
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Bill_Oreilly
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Re: Syringe question [Re: taGyo]
#22011726 - 07/28/15 07:56 PM (8 years, 6 months ago) |
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I just might have to get on that then
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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LoveNaborFuckHater
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Re: Syringe question [Re: taGyo]
#22011727 - 07/28/15 07:56 PM (8 years, 6 months ago) |
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All I do is take a good sized cardboard box, cut the whole top off and cut to arm holes in the side. Then I just tape a bunch of saran wrap over the top so I can look in. Noc'd 15 or so jars this way and never got a contam
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taGyo
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This was my first SAB:

The box my premade jars came from.
They all turned to shit:
Not the SAB's fault though. I went on to make five LCs in that thing that were perfect.
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Bill_Oreilly
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Re: Syringe question [Re: taGyo]
#22011749 - 07/28/15 08:03 PM (8 years, 6 months ago) |
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for some odd reason ive been having really good luck inoculating jars...im like 20/21 as of late..and the 1 contammed jar was the jar that I forgot to put the micropore tape on, so I was noc'ing up the jar withot anything over the holes
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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taGyo
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I just wouldn't take the open-air risk with spores unless I was working with agar
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LoveNaborFuckHater
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Re: Syringe question [Re: taGyo]
#22011817 - 07/28/15 08:15 PM (8 years, 6 months ago) |
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I read on a tek you can no them up outside just after a rain, I don't know how trusting I'd be of this though.
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MagicInMichigan
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Quote:
LoveNaborFuckHater said: I read on a tek you can no them up outside just after a rain, I don't know how trusting I'd be of this though.
(runs outside with umbrella & starts transferring wedges to grain jars) god that would be nice, SABs are a bitch with my monster hands. Tall lanky people problems Sorry for thread jacking, continue on.
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Pastywhyte
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Agar. Grow forever for free. Never buy spores again. Get wicked clones and never have a shitty harvest again. Is as easy as PF tek but far better. Make LC if you like, use clean wedges with fast cloned genetics to make it really kick ass.
I did pf tek once, cloned the fruits and never looked back.
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maddchef
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Got me beat. I never did pf tek.
To pop my cherry I used whole brown rice and made a few cakes.
Then my first monotub I didn't pay attention to quart vs pint and made a tub with 2 bricks coir and 4 pints spawn.
Fucking thing went 4-5 flushes and put out roughly a qp a week, I was booked.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Buck513
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Quote:
Bill_Oreilly said: The thing is i only have that one syringe and really need to make a new one with a print because its from a different batch of very potent PE so i easily would have sacrificed that little spore solution left if i had to in order to make a new syringe with this print..
You got a pe print? Im sensing in the very near future, Bill will get around to writing a tek.
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Bill_Oreilly
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Re: Syringe question [Re: Buck513]
#22012107 - 07/28/15 09:08 PM (8 years, 6 months ago) |
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waiting for my PE outdoor patch to pin..stupid me forgot to take a print off the indoor fruits so now I have to wait.
is a print the very first step in cloning/agar and all that jazz?
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Buck513
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No, not really. Taking at print in hopes of somewhat holding a line of genes is useless. There's hundreds of millions, even billions of spores in a spore print. More spores = larger gene pool.
Agar is extremely easy. I'm fortunate I only grew once before I got my nut up and got into agar. Don't be a fool bill.
Agar, motherfucker. You're gonna realize you should have done it sooner.
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Pastywhyte
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Re: Syringe question [Re: Buck513]
#22012149 - 07/28/15 09:15 PM (8 years, 6 months ago) |
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You can start a no pour tek for about $20. A SAB is the cost of a tote. Small investment to make IMO.
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taGyo
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Bill_Oreilly
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Re: Syringe question [Re: taGyo]
#22013447 - 07/29/15 06:01 AM (8 years, 6 months ago) |
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So lets say i wanted to go that route. Whats the first step?
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Pastywhyte
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Build a SAB. Basically a clear tote with 2 four inch holes cut into it. Then check the link in my sig on easy agar.
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taGyo
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