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Offlinetryptkaloids
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Registered: 02/08/15
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Re: Syringe question [Re: Dr.Satan]
    #22010899 - 07/28/15 05:25 PM (8 years, 6 months ago)

yea man, taGyo knows his shit he's helped a ton of people out a lot! :hug:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
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OfflineLoveNaborFuckHater
That one guy


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Re: Syringe question [Re: Dr.Satan]
    #22010905 - 07/28/15 05:26 PM (8 years, 6 months ago)

If he wanted to keep the potency of the variety he has shouldn't he clone by LC? I was under the impression that if he were to make a syringe out of a print it would just be a MS because that print has a random set of genetics compared to him getting a clone of a potent specimen


--------------------
"They told me drugs were bad, oh man, oh man, they had me fooled"


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OfflinetaGyo
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Re: Syringe question [Re: LoveNaborFuckHater]
    #22010925 - 07/28/15 05:30 PM (8 years, 6 months ago)

:salute:

I shouldn't have lost my temper though. With that thread with the guy with the cake I always wait for a more positive ID but contams won't spread through a dry verm layer into another jar. It's impossible with the dry verm layer.

Quote:

spacechildo said:
Quote:

taGyo said:
As Spacechild said, making an LC from an MS syringe bought from a sponsor is very tricky.




heh, I didn't say it here but its my stance on it yeah. and especially if its just a random syringe you traded/bought.



Guess I'm a mind reader :wink:

Quote:

LoveNaborFuckHater said:
If he wanted to keep the potency of the variety he has shouldn't he clone by LC? I was under the impression that if he were to make a syringe out of a print it would just be a MS because that print has a random set of genetics compared to him getting a clone of a potent specimen



Clone is ideal, from that spore syringe he could even grow bunk PE but there are so many possibilities from a spore.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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OfflineMachiavelliavore
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Re: Syringe question [Re: Bill_Oreilly]
    #22010933 - 07/28/15 05:32 PM (8 years, 6 months ago)

There are a bunch of stories about mycologists accidentally growing cubes cause a few spores were around when trying to grow exotics.

It's probably sterile with the exception of the spores that are left in it after you empty it.

No reason to be wreckless with a project that will span months, boil dat water and suck'r up.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinetryptkaloids
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Re: Syringe question [Re: taGyo]
    #22010949 - 07/28/15 05:34 PM (8 years, 6 months ago)

what if the contam got in there through a wet spot in the dry verm layer? could it transfer then?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflinetaGyo
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Re: Syringe question [Re: tryptkaloids]
    #22010975 - 07/28/15 05:38 PM (8 years, 6 months ago)

Very, extremely unlikely. Probably less likely then a dry verm layer as the water would saturate the medium and make the spores heavier.

You'd have to open it up in your grow area and push some of the contam into the holes and past the dry verm layer of the second brf jar to contam it through the air. We use dry verm because it's very good at what it does, catching anything microscopic that happens to float down.

That or a gust of wind would have to pick up the spores from the first BRF jar and magically transport them over to the second while moving the second jars dry verm.

:thumbup:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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OfflineLoveNaborFuckHater
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Re: Syringe question [Re: taGyo]
    #22011005 - 07/28/15 05:44 PM (8 years, 6 months ago)

Quote:

taGyo said:
Clone is ideal, from that spore syringe he could even grow bunk PE but there are so many possibilities from a spore.



So why throw out what solution he has in his syringe if the new print will result in such a variety of genetics that they'll likely just end up the same as they are MS and not LC? I'm wondering why take from a print because I used MS and my cubes were so weak an 8th barely did anything. Is him using a print v. MS not essentially the same because the print carries just as much variety as an MS will?


--------------------
"They told me drugs were bad, oh man, oh man, they had me fooled"


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OfflinetaGyo
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Re: Syringe question [Re: LoveNaborFuckHater]
    #22011012 - 07/28/15 05:45 PM (8 years, 6 months ago)

Quote:

LoveNaborFuckHater said:
Quote:

taGyo said:
Clone is ideal, from that spore syringe he could even grow bunk PE but there are so many possibilities from a spore.



So why throw out what solution he has in his syringe if the new print will result in such a variety of genetics that they'll likely just end up the same as they are MS and not LC? I'm wondering why take from a print because I used MS and my cubes were so weak an 8th barely did anything. Is him using a print v. MS not essentially the same because the print carries just as much variety as an MS will?



If he's not working with LC he's most likely not working with clones yet.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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OfflineLoveNaborFuckHater
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Re: Syringe question [Re: taGyo]
    #22011026 - 07/28/15 05:48 PM (8 years, 6 months ago)

I just feel it would be a waste of time and solution if he wants to get that potency he would probably have to clone


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"They told me drugs were bad, oh man, oh man, they had me fooled"


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OfflinetaGyo
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Re: Syringe question [Re: LoveNaborFuckHater]
    #22011033 - 07/28/15 05:50 PM (8 years, 6 months ago)

I agree :thumbup:

But you have to suggest what people are comfortable with. I work with agar and take clones.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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OfflineLoveNaborFuckHater
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Re: Syringe question [Re: taGyo]
    #22011053 - 07/28/15 05:54 PM (8 years, 6 months ago)

I don't want other people to waste a multi-month project if they only have one going on MS. It was a learning experience for me but next time I get around to growing I'm definitely going to try cloning with agar


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OfflinetaGyo
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Re: Syringe question [Re: LoveNaborFuckHater]
    #22011079 - 07/28/15 06:01 PM (8 years, 6 months ago)

:kenthumbup:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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InvisibleBill_Oreilly
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Re: Syringe question [Re: taGyo]
    #22011104 - 07/28/15 06:05 PM (8 years, 6 months ago)

So I shouldn't do the print in hopes to get a potency pretty close to the PE they grow into?

I thought if I took a print of the PE I have, then the genetics would be MS, but also close to what the potency of the mushroom I printed.

if that's not the case im just going to say fuck the whole printing thing and just use the syringe up and just take a print later just for the reason of not having to buy my own.


--------------------
Something there is mysteriously formed,
Existing before Heaven and Earth,
Silent, still, standing alone, unchanging,
All-pervading, unfailing,
I do not know its name; I call it tao.
If forced to give it a name, I call it
Great (ta). Being great, it flows out;
Flowing out means far-reaching;
Being far-reaching, it is said to return.


It's just a shot away..


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OfflinetaGyo
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Re: Syringe question [Re: Bill_Oreilly]
    #22011147 - 07/28/15 06:12 PM (8 years, 6 months ago)

Okay here's something I recently learned so I hope I'm explaining it right:

Lets say you take a print from Flush A. In that print you have Flush A genetics, Flush B genetics and then a shit ton of other spores. If you happen to get the same potency from the next PE and you print that eventually, after many, many generations of continuing this, you will create a spore print with a base potency. This is the process of training a strain to do a certain thing, for instance most GT and B+ prolifically fruits.

It's just a shot in the dark as to whether you'll find that same exact potency, more potent or completely bunk which is why clone >.

But yes, in theory, if you keep taking prints from a potent cube you will have a higher chance of creating potent cubes through generation after generation. This is also how you breed for leucistic traits and whatnot. Print the leucistic ones until you get all white shrooms.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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OfflineLoveNaborFuckHater
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Re: Syringe question [Re: Bill_Oreilly]
    #22011170 - 07/28/15 06:16 PM (8 years, 6 months ago)

If you still have the fruits you took the print from you could just clone and transfer straight to a jar or use those outdoor fruits in your other thread if they end up being PE (correct me if I'm wrong).


--------------------
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OfflinetaGyo
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Re: Syringe question [Re: LoveNaborFuckHater]
    #22011178 - 07/28/15 06:17 PM (8 years, 6 months ago)

:kenthumbup:

I'd put it on agar first though but you can go straight to grain with success, it'll be a bit slow though.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Offlinetryptkaloids
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Re: Syringe question [Re: Bill_Oreilly]
    #22011206 - 07/28/15 06:20 PM (8 years, 6 months ago)

I'm assuming that by adding cloning to the process you can cut down the time it takes to do that.. if you take tissue from a really tall fruit then the fruits will come out tall. if you print from a rally potent clone that has been isolated over and over again then there will be a higher percentage of the potent genes in it. atleast this is my basic understanding of biology. 
please correct me if any of this sounds incorrect


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineLoveNaborFuckHater
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Re: Syringe question [Re: taGyo]
    #22011252 - 07/28/15 06:27 PM (8 years, 6 months ago)

Quote:

taGyo said:
:kenthumbup:

I'd put it on agar first though but you can go straight to grain with success, it'll be a bit slow though.



Would cutting out a bigger piece out the fruit make any difference in speed? Someone also told me the other day that there can be differences depending on where you cut from


--------------------
"They told me drugs were bad, oh man, oh man, they had me fooled"


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Offlinetryptkaloids
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Re: Syringe question [Re: LoveNaborFuckHater]
    #22011288 - 07/28/15 06:32 PM (8 years, 6 months ago)

i don't see why not, often the amount of inoculant is pretty strong in determining speed of colonisation. at least with liquids. I think it has to do with inoculation points


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflinetaGyo
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Re: Syringe question [Re: LoveNaborFuckHater]
    #22011307 - 07/28/15 06:35 PM (8 years, 6 months ago)

Quote:

tryptkaloids said:
I'm assuming that by adding cloning to the process you can cut down the time it takes to do that.. if you take tissue from a really tall fruit then the fruits will come out tall. if you print from a rally potent clone that has been isolated over and over again then there will be a higher percentage of the potent genes in it. atleast this is my basic understanding of biology. 
please correct me if any of this sounds incorrect



:kenthumbup:

Takes many generations though.

Quote:

LoveNaborFuckHater said:
Quote:

taGyo said:
:kenthumbup:

I'd put it on agar first though but you can go straight to grain with success, it'll be a bit slow though.



Would cutting out a bigger piece out the fruit make any difference in speed? Someone also told me the other day that there can be differences depending on where you cut from



A slight difference :thumbup:. It will definitely be faster but depending on how big the piece not by much. The mycelium has to re-orient itself to colonization and then "leap-off" the clone tissue to begin colonization. First flush is best for this as subsequent flushes have much less vigorous mycelium.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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