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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Syringe question
#22010646 - 07/28/15 04:29 PM (8 years, 6 months ago) |
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Is the inside of a bought-spore syringe sterile?
I want to try to make my own, and I have a syringe with like 10cc left of solution..if I squirt that out and flame-sterilize the tip...am I good to go?
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 5 hours
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i'm confused.. you have spore solution but you want to throw it out to make another syringe?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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I would boil some water and suck it up and squirt it out into the sink a few times to be sure the inside is completely sterile. I've always followed this tek: http://www.shroomery.org/forums/showflat.php/Number/12098357/fpart/all/vc/1
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 5 hours
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why don't you use the left over solution on more cakes/grains? after you are out of solution boil water and suck it up into the syringe and discard a couple times. this will sterilize your syringe and needle. this is all described in the making a syringe tek posted in the mushroom info section
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Well i was going to if i found out from tou guys that i could without making it non sterile. The thing is i only have that one syringe and really need to make a new one with a print because its from a different batch of very potent PE so i easily would have sacrificed that little spore solution left if i had to in order to make a new syringe with this print..its the only one i have left of this because i forgot to make others so i really want to save these genes
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Quote:
Psilosoulful said: I would boil some water and suck it up and squirt it out into the sink a few times to be sure the inside is completely sterile. I've always followed this tek: http://www.shroomery.org/forums/showflat.php/Number/12098357/fpart/all/vc/1
This is all I do and it's been proven to work so far testing the solution on agar dishes. That's always the best move, make sure you succeeded at sterile procedure by testing the solution on an agar dish.
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Re: Syringe question [Re: taGyo]
#22010716 - 07/28/15 04:47 PM (8 years, 6 months ago) |
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Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what im used to doing
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Quote:
Bill_Oreilly said:
Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what in used to doing 
don't do LC, a MS syringe is far more simple to make plus less room for error. Every TC I've come across says don't fuck with LC it's a waste of time.
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Edited by Dr.Satan (07/28/15 04:51 PM)
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Quote:
Bill_Oreilly said:
Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what in used to doing 
Ahh.
So you have an empty store-bought syringe, correct? I would use that boil method they talked about earlier. I've used it twice and it was good, I do my syringes different now though.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Syringe question [Re: Dr.Satan]
#22010740 - 07/28/15 04:50 PM (8 years, 6 months ago) |
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Quote:
Dr.Satan said:
Quote:
Bill_Oreilly said:
Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what in used to doing 
don't do LC, a MS syringe is far more simple to make.
Simpler then Karo and water?
LOL, how simple are you?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)


Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Re: Syringe question [Re: taGyo]
#22010746 - 07/28/15 04:52 PM (8 years, 6 months ago) |
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Quote:
taGyo said:
Quote:
Bill_Oreilly said:
Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what in used to doing 
Ahh.
So you have an empty store-bought syringe, correct? I would use that boil method they talked about earlier. I've used it twice and it was good, I do my syringes different now though.
Its a spore syringe bought from a sponser woth about 20cc left in it
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Re: Syringe question [Re: taGyo]
#22010753 - 07/28/15 04:53 PM (8 years, 6 months ago) |
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Quote:
taGyo said:
Quote:
Dr.Satan said:
Quote:
Bill_Oreilly said:
Quote:
taGyo said: Why not just make an LC?
Most syringes are made by people like you and me, I got 5 APE jars that were extremely contaminated. User error occurs, I'd shoot it on agar before anything.
I dont know how to make an LC..never really researched it. I just like using syringes because its what in used to doing 
don't do LC, a MS syringe is far more simple to make.
Simpler then Karo and water?
LOL, how simple are you?
Look, I've never made an LC syringe because the trusted cultivators on here say it's a waste of time. You are the only person I've ever seen on here saying LC is a good idea. So, not to be rude, but I'm going to continue believing it is probably a waste of time. All you need to do is boil water and have a spore print to make a MS syringe.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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most bought syringes have a fuck ton of spores in them, sterilize a small jar with water in it and shoot the 10 or 20 cc into it. now you have a jar full of spore water and an empty syringe so you can do the boiling water to syringe thing.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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That's what I thought it was lol.
If you're looking to extend it I would really do an LC or Agar.
@Dr.Satan Which TC? Lol. I've been around the block, LCs aren't a waste of time with a clean syringe, it's a good way to extend a life cycle.
I'm more of an agar man myself but if that's not an option then I would suggest an LC, .25 cc from that syringe can make a whole viable, clean LC with the right technique.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Re: Syringe question [Re: taGyo]
#22010816 - 07/28/15 05:05 PM (8 years, 6 months ago) |
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Quote:
spacechildo said: most bought syringes have a fuck ton of spores in them, sterilize a small jar with water in it and shoot the 10 or 20 cc into it. now you have a jar full of spore water and an empty syringe so you can do the boiling water to syringe thing. 
This seems like the best option for OP. I mean there's little to no way to fuck that up.
Quote:
taGyo said:
@Dr.Satan Which TC? Lol. I've been around the block, LCs aren't a waste of time with a clean syringe, it's a good way to extend a life cycle.
It's not like I remember the TC's usernames, but either way they say LC has far more room for error sterilization wise then making a MS syringe does. I know LC can work given the right technique, but is it really worth all the trouble in comparison?
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SteveRogers
gandy dancer



Registered: 10/24/06
Posts: 3,450
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Quote:
Bill_Oreilly said: so i really want to save these genes
agar and slants FTW. it will change you life man.
-------------------- "General, I am loyal to nothing......except The Dream"
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Syringe question [Re: Dr.Satan]
#22010837 - 07/28/15 05:09 PM (8 years, 6 months ago) |
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Okay.
I'm sorry, I shouldn't have snapped and been rude.
Here's the breakdown of LC: It is something that is very easy to mess up which is why most people don't recommend it to new members. Bill has been around the block so I think he can comprehend and understand what it takes to make a clean LC.
As Spacechild said, making an LC from an MS syringe bought from a sponsor is very tricky. My first LC, and Spacechild will confirm this, I made was before I even saw my first mushroom. they were all 100% clean and the tub they were used on had a flush so full I had to break mushrooms to harvest it.
LC is a very viable way to extend a clone, syringe, or Liquid Innoculant. You can actually take a part of the LC and squeeze it into more LC water and extend it AGAIN.
Now here is where the difficulty comes in to play: Without agar work to make sure that syringe is clean you could also be breeding a contam. LC is very tricky in the fact that if you are breeding a contam you will most likely not see it so you have to test it on either a jar or on agar.
With proper testing and control, you'll be fine to do an LC. With 20cc of a syringe, that's A LOT of LC.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Syringe question [Re: taGyo]
#22010851 - 07/28/15 05:13 PM (8 years, 6 months ago) |
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Quote:
taGyo said: As Spacechild said, making an LC from an MS syringe bought from a sponsor is very tricky.
heh, I didn't say it here but its my stance on it yeah. and especially if its just a random syringe you traded/bought.
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Re: Syringe question [Re: taGyo]
#22010858 - 07/28/15 05:16 PM (8 years, 6 months ago) |
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Quote:
taGyo said: Okay.
I'm sorry, I shouldn't have snapped and been rude.
Here's the breakdown of LC: It is something that is very easy to mess up which is why most people don't recommend it to new members. Bill has been around the block so I think he can comprehend and understand what it takes to make a clean LC.
As Spacechild said, making an LC from an MS syringe bought from a sponsor is very tricky. My first LC, and Spacechild will confirm this, I made was before I even saw my first mushroom. they were all 100% clean and the tub they were used on had a flush so full I had to break mushrooms to harvest it.
LC is a very viable way to extend a clone, syringe, or Liquid Innoculant. You can actually take a part of the LC and squeeze it into more LC water and extend it AGAIN.
Now here is where the difficulty comes in to play: Without agar work to make sure that syringe is clean you could also be breeding a contam. LC is very tricky in the fact that if you are breeding a contam you will most likely not see it so you have to test it on either a jar or on agar.
With proper testing and control, you'll be fine to do an LC. With 20cc of a syringe, that's A LOT of LC.
I just checked out your journal and you're far more experienced with this hobby then I would of guessed. I will consider you a trusted cultivator from here on out
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