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ChemClear
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Registered: 06/16/15
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Noob Question On Colonisation Times Using LC on WBS
#22008289 - 07/28/15 07:43 AM (8 years, 6 months ago) |
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Hi Guys,
This is my first time posting on the forum, and admittedly this is a pretty noob question.
Here's the background:
This is my first shroom grow. Spores are super expensive and take months to reach my country. The chances of them arriving through our postal system are small too. This isn't a result of legality, rather it's just an indication of how awful our postal system is.
Needless to say I was thrilled when a friend gave me a B+ cubensis syringe.
None of the sterile techniques sounded particularly daunting, so I opted to go with LC spawned onto WBS for a monotub setup. (this choice was made as it seems like the best route to get the most out of a single syringe)
The LC took 5 days to start showing signs of successful inoculation.
Samples were taken from each of the 3 LC jars and tested on agar - no infection after 5 days. Healthy Mycelium growth.
I then inoculated 12 jars with the LC that was the most vigorous on the agar, on wild bird seed (red & yellow millet).
Within 2 days visible mycelium growth was forming on the bottom of the jar.
I'm now on day 12 since jar inoculation. No signs of infection, but mycelium growth appears to have slowed down. Jars are all incubating in a water bath with a constant temp of 24C.
So here's the crux of the matter - how long does it usually take to colonise a jar of WBS using LC? Am I being impatient?
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bakenast
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: ChemClear]
#22010257 - 07/28/15 03:14 PM (8 years, 6 months ago) |
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shake the jars up! they should recover fine if there isint a problem and colonise the rest..
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: bakenast]
#22010424 - 07/28/15 03:43 PM (8 years, 6 months ago) |
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Depends on how big a jar and how much lc is used. 4-20 days.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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ChemClear
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: maddchef]
#22012997 - 07/29/15 01:30 AM (8 years, 6 months ago) |
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Ok, I gave the jars a shake 2 days ago. Which seems to have sent them for a knock. I haven't seen much growth since. Perhaps the m network is busy recovering? I'm inoculating another 6 jars this evening and prepping another LC Jar. If it helps anyone I'm using 500ml jars, which I think is fairly close to a pint. Should I be going smaller? 500ml Jars were the smallest I could find. Is it a good idea to try bags instead? Thanks in advance for the help.
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HeadPot
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: ChemClear]
#22013015 - 07/29/15 01:41 AM (8 years, 6 months ago) |
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Jars are fine, they stall sometimes i got few myself (WBS) i found that if i add 5g of gypsum during the soak and then for the simmer jars colonise twice faster and they do not stall. Try to turn the stalled ones upside down i found that to help sometimes cheers
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Machiavelliavore
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: ChemClear]
#22013021 - 07/29/15 01:42 AM (8 years, 6 months ago) |
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1L jars are nice for grain spawn.
Recovery from a shake takes 1-3 days typically, so you want to do it when you have enough colonized grain to inoculate the whole jar. I haven't used LC, but typically I think you shake imediately after inoculating with a generous quantity of LC to distribute it, removing the need for a later shake that will induce a recovery time.
They should be colonized within 10 days unless you waited too long to shake'm.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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ChemClear
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: Machiavelliavore]
#22013035 - 07/29/15 01:50 AM (8 years, 6 months ago) |
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Thanks Guys. I've got about 12 Jars free, So I'm definitely going to add some gypsum to the next simmer of WBS. I'll keep you updated with the progress. Blown away by the level of support on this forum.
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Chk
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: ChemClear]
#22013170 - 07/29/15 03:07 AM (8 years, 6 months ago) |
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Well, if you have problems obtaining spores i would advice keeping 2 or 3 drops in your syringue to start some invitro grow and make some very clean print.
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Machiavelliavore
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: Chk]
#22013240 - 07/29/15 03:53 AM (8 years, 6 months ago) |
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He/she could also just save some of his LC to make new LC's with. Also if he/she has agar (sounds like he does,) open-air prints shouldn't be an issue.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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ChemClear
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: Chk]
#22013317 - 07/29/15 04:58 AM (8 years, 6 months ago) |
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Could you link me to invitro techniques that you've had success with please. I've got about 5cc of spore syringe left and I'd like to make the most of it. Spore prints are definitely on the cards once I've managed to fruit. I opted for LC for the simple reason that it seems to be the best way to extend your spores.
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Machiavelliavore
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: ChemClear]
#22013326 - 07/29/15 05:06 AM (8 years, 6 months ago) |
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Most of them are essentially making a PF cake in a quart jar or container, increasing the GE to fruit it, and then transfering the fruit to print in an airbox.
If you're already doing agar work, you can clean up an open air print easily and don't need to bother with invitro growing.
You can also do LC to LC transfers, which supposedly you can keep going almost forever from a master LC.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: Machiavelliavore] 1
#22013338 - 07/29/15 05:13 AM (8 years, 6 months ago) |
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Lc inoculated with spores. LC to grains show growth then stall. Grains shaken don't recover. Diagnosis, bacteria.
Without pics of the LC, and the jars its hard to say exactly where the vector is. Could be the syringe or the grains or both. Also stop incubating. It gives the bacteria the edge over the mycelium.
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ChemClear
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: Pastywhyte]
#22013364 - 07/29/15 05:23 AM (8 years, 6 months ago) |
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Quote:
Pastywhyte said: Lc inoculated with spores. LC to grains show growth then stall. Grains shaken don't recover. Diagnosis, bacteria.
Without pics of the LC, and the jars its hard to say exactly where the vector is. Could be the syringe or the grains or both. Also stop incubating. It gives the bacteria the edge over the mycelium.
Thanks! That's excellent advice. I'll snap some pics for you this evening when I'm back home.
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MikeBearPig
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Registered: 07/15/14
Posts: 2,319
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: ChemClear] 1
#22013368 - 07/29/15 05:24 AM (8 years, 6 months ago) |
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Quote:
ChemClear said:
Quote:
Pastywhyte said: Lc inoculated with spores. LC to grains show growth then stall. Grains shaken don't recover. Diagnosis, bacteria.
Without pics of the LC, and the jars its hard to say exactly where the vector is. Could be the syringe or the grains or both. Also stop incubating. It gives the bacteria the edge over the mycelium.
Thanks! That's excellent advice. I'll snap some pics for you this evening when I'm back home.
I had this happen to 1 jar recently.. Was all mycelium, shook it and never came back. I let it sit for 3 weeks so I knew what it was. I think it was yeast.
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hamloaf
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: Pastywhyte]
#22013376 - 07/29/15 05:26 AM (8 years, 6 months ago) |
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Quote:
Pastywhyte said: Lc inoculated with spores. LC to grains show growth then stall. Grains shaken don't recover. Diagnosis, bacteria.
Without pics of the LC, and the jars its hard to say exactly where the vector is. Could be the syringe or the grains or both. Also stop incubating. It gives the bacteria the edge over the mycelium.

24C is a good temp though. Incubation by incubation chamber is ok if you live in a region where it's constantly colder than acceptable range for the colonization of specie of mushroom in question. Otherwise it's all incubation.
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ChemClear
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: Machiavelliavore]
#22013388 - 07/29/15 05:30 AM (8 years, 6 months ago) |
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You're going to have to forgive me. I'm not sure what GE stands for and I'm battling to get a definition on the forums. Care to enlighten me?
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hamloaf
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Re: Noob Question On Colonisation Times Using LC on WBS [Re: ChemClear] 1
#22013390 - 07/29/15 05:31 AM (8 years, 6 months ago) |
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GE stands for Gas Exchange.
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