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dirttoldfart
Stranger
Registered: 07/12/15
Posts: 38
Last seen: 8 years, 2 months
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My first attempt at mushroom cultivation
#22004881 - 07/27/15 02:37 PM (8 years, 6 months ago) |
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So this is my first time trying to grow shrooms, and I don't really know what I'm doing wrong. I followed this tek exactly:
http://www.shroomery.org/forums/showflat.php/Number/15223324/fpart/1/vc/1
I inoculated 12 jars, 4 with Cambodian, 4 with B+, and 4 with Golden Teacher. This was 10 days ago, and there is no sign of contamination, but also no sign of mycelium. I keep them on a shelf in a closet, with temperature ranging from 79F in the day to 74F in the night. Am I just not being patient enough, or is there something wrong? I bought the spore syringes from a trusted and well-reviewed vendor.
Here are some pictures of my jars today:

(I don't keep them stacked like they are in the first picture, I just did that to make them more visible in the picture)
Edited by dirttoldfart (07/27/15 02:44 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22004901 - 07/27/15 02:40 PM (8 years, 6 months ago) |
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Only been 10 days. They can take 2-30 days to germinate sometimes
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blackout


Registered: 07/16/00
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Re: My first attempt at mushroom cultivation [Re: bodhisatta]
#22005010 - 07/27/15 02:59 PM (8 years, 6 months ago) |
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If you are sure you let them cool enough I would wait it out. 10 days is not much.
Instead of 3 different cube strains I would have advised getting a sclerotia / stone producing strain from 1 particular sponsor (who I cannot name) if you mention you are a shroomery member and ask nicely they will typically throw in a free cube strain of their choice for free. Research and you would fine out who, maybe more than one does it.
This way if your cubensis fail you have the sclerotia to fall back on. You can grow sclerotia on whole grain rice which can be steamed.
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Re: My first attempt at mushroom cultivation [Re: blackout]
#22005126 - 07/27/15 03:28 PM (8 years, 6 months ago) |
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I know the waiting game sucks.
Try this, give them a little light. Its a common misconception that incubation requires absolute darkness.. When in fact the actually need a little light to get going! Give it a shot. Don't use sunlight (direct sun is NOT good for your jars) Us indirect light. Just open the closet door for like 4-8 hours a day. I'm using this method also (indirect light) and I always have mycelium growth on day 3 or 4.
I'm following the the same tek as you from the link in my signature.
Best of luck buddy, report back ya?
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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ShroominMe
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Re: My first attempt at mushroom cultivation [Re: JacobStorm]
#22005147 - 07/27/15 03:33 PM (8 years, 6 months ago) |
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Yeah it's frustrating reading all those posts about seeing growth in three days and you look... day after day... and nothing.
You should have some spore syringe solution left over. Why not inoculate a few more jars as a backup. Plus it'll give you something to do.
Good luck!
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Re: My first attempt at mushroom cultivation [Re: ShroominMe]
#22005330 - 07/27/15 04:13 PM (8 years, 6 months ago) |
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This hobby rewards the dedicated, the diligent and the patient.
I learned that the hard way..
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
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Re: My first attempt at mushroom cultivation [Re: ShroominMe]
#22005337 - 07/27/15 04:16 PM (8 years, 6 months ago) |
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Patience. This shit is all about patience. You're in the right timeline so far.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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dirttoldfart
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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22005390 - 07/27/15 04:32 PM (8 years, 6 months ago) |
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Thanks for all the replies, I'll let the jars sit for a week or two and report back then.
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22005427 - 07/27/15 04:39 PM (8 years, 6 months ago) |
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Also try not handle them TOO much. Your a germ factory try to let them be.
And don't tip the jars on their side, always look at them in a vertical sense. You don't wanna go shifting or moving around that dry-vermiculite-contaminate-barrier.
When you were inoculating, and putting the needle through the inoculation hole in the jar, and didn't have the needle its self directly against the side of the jar, theres a good chance what happened was the liquid spores soaked into more of the substrate towards the middle rather than the very OUTSIDE of the substrate, where we look for mycelium growth. (it happens) So patients, patients and more patients!
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Re: My first attempt at mushroom cultivation [Re: JacobStorm]
#22005496 - 07/27/15 04:56 PM (8 years, 6 months ago) |
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Quote:
JacobStorm said: This hobby rewards the dedicated, the diligent and the patient.
I learned that the hard way..
--------------------

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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: My first attempt at mushroom cultivation [Re: Dr.Satan]
#22006766 - 07/27/15 08:36 PM (8 years, 6 months ago) |
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If you did your filter right you can touch it all you want. But yeah, don't tilt them if you can help it.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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jesuisravi
The Old Noob



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Posts: 260
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Re: My first attempt at mushroom cultivation [Re: 36fuckin5]
#22006857 - 07/27/15 08:57 PM (8 years, 6 months ago) |
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I feel your pain.
-------------------- Most of my beliefs I acquired from my father and from John Wayne, and anything that wasn't ultra tough and ultra cool was to me ultra embarrassing. In fact, I lived in a state of near continuous embarrassment, never measuring up to the ridiculous standards I had accepted without question, applied to a framework of expectations neither I nor anyone else could meet.--J C Amberchele almost nothing important that ever happens to you happens because you engineer it. Destiny has no beeper; destiny always leans trenchcoated out of an alley with some sort of 'psst' that you usually can't even hear because you're in such a rush to or from something important you've tried to engineer. ” ― David Foster Wallace, Infinite Jest
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dirttoldfart
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Registered: 07/12/15
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Last seen: 8 years, 2 months
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So i glanced at my jars this morning and it looks like one jar may have developed some kind of contamination.

I'm looking at the small patch towards the top in the middle that looks greenish.
I moved it away from the other jars to be safe. The rest of the jars still show no signs of growth.
Edited by dirttoldfart (07/28/15 01:35 PM)
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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You did the right thing moving it away from the others.
I hope it's not a contame but it does kinda look green...
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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Psilosoulful

Registered: 09/05/14
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Last seen: 1 year, 1 month
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Quote:
dirttoldfart said: So i glanced at my jars this morning and it looks like one jar may have developed some kind of contamination.

I'm looking at the small patch towards the top in the middle that looks greenish.
That's the beginnings of trich. And the bottom of the jar looks dark, I'm not sure if thats a shadow or black mold? Either way, I'd toss it.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
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Quote:
dirttoldfart said: So i glanced at my jars this morning and it looks like one jar may have developed some kind of contamination.

I'm looking at the small patch towards the top in the middle that looks greenish.
I moved it away from the other jars to be safe. The rest of the jars still show no signs of growth.
I'd just wait it out 
You'll know soon enough.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Dr.Satan
Mad Professor



Registered: 06/26/15
Posts: 2,182
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Re: Update 1 [Re: taGyo]
#22010648 - 07/28/15 04:29 PM (8 years, 6 months ago) |
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Quote:
taGyo said:
Quote:
dirttoldfart said: So i glanced at my jars this morning and it looks like one jar may have developed some kind of contamination.

I'm looking at the small patch towards the top in the middle that looks greenish.
I moved it away from the other jars to be safe. The rest of the jars still show no signs of growth.
I'd just wait it out 
You'll know soon enough.
If you decide to wait it out make sure you do so outside. Any suspected contams I get I just move them outdoors until I can be sure. If anything other then pure white myc starts to grow in your jars you can be pretty certain it's a contam. That has a greenish appearance so I would say it's a definite contam.
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taGyo
Strainiac/AMU



Registered: 10/16/14
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It's not going to pass the dry verm layer to infect everything lol.
You're being overly cautious. I fruit tubs next to contamed tubs and they don't cross-contam because of the polyfill.
But do what you want
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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dirttoldfart
Stranger
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Last seen: 8 years, 2 months
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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22010689 - 07/28/15 04:40 PM (8 years, 6 months ago) |
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Decided just to get rid of the contaminated jar. I took it outside and dumped the cake out, and then just because I was curious I broke the cake apart. The green growth looked fuzzy like some kind of mold and it continued well into the center of the cake.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22012578 - 07/28/15 10:48 PM (8 years, 6 months ago) |
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I just skimmed, but I saw something about black mold. If you see any black or purple molds, you DO NOT fuck around. PC them immediately, don't open the jar, double bag them and throw them the fuck out. Black and purple molds can kill.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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dirttoldfart
Stranger
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Posts: 38
Last seen: 8 years, 2 months
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Re: My first attempt at mushroom cultivation [Re: 36fuckin5]
#22012670 - 07/28/15 11:16 PM (8 years, 6 months ago) |
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No black mold, just a shadow from my camera on one of the jars in a picture of some greenish contam.
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Angel_Dander
Entheogenist


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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22015608 - 07/29/15 03:31 PM (8 years, 6 months ago) |
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No tape on the holes???
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: My first attempt at mushroom cultivation [Re: Angel_Dander]
#22015614 - 07/29/15 03:32 PM (8 years, 6 months ago) |
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If you have a dry verm layer the tape is 100% useless.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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dirttoldfart
Stranger
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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22015776 - 07/29/15 04:01 PM (8 years, 6 months ago) |
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Update:
Checked on the jars this afternoon, and one more jar looks like it has some kind of contamination.

I don't know how visible it is in that picture, but I'm talking about the greenish spot on the top right.
Still no signs of mycelium growth on the other 10 jars.
Edited by dirttoldfart (07/29/15 04:02 PM)
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taGyo
Strainiac/AMU



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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22015783 - 07/29/15 04:03 PM (8 years, 6 months ago) |
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What is your procedure?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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dirttoldfart
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Re: My first attempt at mushroom cultivation [Re: taGyo]
#22016391 - 07/29/15 06:20 PM (8 years, 6 months ago) |
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I followed this tek
http://www.shroomery.org/forums/showflat.php/Number/15223324/fpart/1/vc/1
Since I was using a pot with tight fitting lid to sterilize, I could only fit 6 jars at a time so the first 6 jars were inoculated about 12 hours before the second six. I (stupidly) inoculated the first 6 without a still air box, but used one for the second set of jars. However, of the two jars that became contaminated in the last couple days, 1 of them was inoculated in the box, and the other was not.
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taGyo
Strainiac/AMU



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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22016424 - 07/29/15 06:26 PM (8 years, 6 months ago) |
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Try 24 hour cool down. You PC with foil, right?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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dirttoldfart
Stranger
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Re: My first attempt at mushroom cultivation [Re: taGyo]
#22016627 - 07/29/15 07:03 PM (8 years, 6 months ago) |
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Yup, steam sterilize with foil and then leave the jars in the pot with the lid closed until it's time to inoculate. I let them cool until they're cooled to room temperature, which was about 5-7 hours. Do you think it could be that they were still warm enough on the inside to kill spores that I injected?
Edited by dirttoldfart (07/29/15 07:03 PM)
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taGyo
Strainiac/AMU



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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22016638 - 07/29/15 07:05 PM (8 years, 6 months ago) |
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Yes.
24 hours
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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ShroominMe
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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22016711 - 07/29/15 07:20 PM (8 years, 6 months ago) |
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Quote:
dirttoldfart said: Yup, steam sterilize with foil and then leave the jars in the pot with the lid closed until it's time to inoculate. I let them cool until they're cooled to room temperature, which was about 5-7 hours. Do you think it could be that they were still warm enough on the inside to kill spores that I injected?
No. If it's room temperature on the outside the core will only be a few degrees warmer. Jars have relatively little mass.
If you're curious next time you prepare some jars use a meat thermometer to check one and you will see how small the temperature fluctuation is through the jar mass.
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taGyo
Strainiac/AMU



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Re: My first attempt at mushroom cultivation [Re: ShroominMe]
#22016717 - 07/29/15 07:21 PM (8 years, 6 months ago) |
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Quote:
ShroominMe said:
Quote:
dirttoldfart said: Yup, steam sterilize with foil and then leave the jars in the pot with the lid closed until it's time to inoculate. I let them cool until they're cooled to room temperature, which was about 5-7 hours. Do you think it could be that they were still warm enough on the inside to kill spores that I injected?
No. If it's room temperature on the outside the core will only be a few degrees warmer. Jars have relatively little mass.
If you're curious next time you prepare some jars use a meat thermometer to check one and you will see how small the temperature fluctuation is through the jar mass.
That's bad advice to give to someone having germination issues.
Always start with the basics and then you can cut shit down. Do you think I wait 24 hours to inject my jars? No but would I suggest someone to do the same as I? No.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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dirttoldfart
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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22027836 - 08/01/15 10:57 AM (8 years, 5 months ago) |
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So it's been a little more than two weeks since inoculation. So far 2 jars have become contaminated, and not a single jar is showing any sign of mycelium growth. Should I just keep waiting? It seems weird that by now not even one jar would be showing signs of growth, which makes me think something is wrong. Any ideas?
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taGyo
Strainiac/AMU



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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22027942 - 08/01/15 11:10 AM (8 years, 5 months ago) |
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Toss them and wait 24 hour cool down. Also, make sure you're only shooting .25 cc a hole. Are you making it per the video:
http://mushroomvideos.com/
Are you flaming your needle?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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dirttoldfart
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Re: My first attempt at mushroom cultivation [Re: taGyo]
#22028201 - 08/01/15 12:39 PM (8 years, 5 months ago) |
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Yup, flamed the needle between every jar.
Edited by dirttoldfart (08/01/15 12:41 PM)
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taGyo
Strainiac/AMU



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Re: My first attempt at mushroom cultivation [Re: dirttoldfart]
#22028223 - 08/01/15 12:45 PM (8 years, 5 months ago) |
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Quote:
taGyo said: Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation. Don't wipe your needle with alcohol after flame sterilization:
Quote:
swatt_haze said: wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.
Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.
Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar. Grains is 1CC as well.
After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.
If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.
Anything in there you're not doing?
Maybe you got a contamed syringe. I would e-mail who you got it from and tell them you can't identify anything except mold under your microscope.
Remember, they will ignore your refund request if you mention cultivation. Spores are sold for microscopic use.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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