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OfflineDocG
Hesco cobra

Registered: 07/09/15
Posts: 28
Last seen: 5 years, 10 months
Cloning question
    #22004161 - 07/27/15 11:58 AM (8 years, 6 months ago)

I plan on starting a new grow by cloning some of my pins. If I do so my questions are what tek is best for cloning and how should I go about the actually cloning.


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Friendly Reminder

66,000 mph. That is how fast we are hurdling through space.
1,600,000 miles. That is how far we travel every day.
All while spinning at around 1000 mph.


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OfflineYaMoonSun
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Re: Cloning question [Re: DocG]
    #22004194 - 07/27/15 12:08 PM (8 years, 6 months ago)



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Offlinethebitterbuffalo26
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Re: Cloning question [Re: YaMoonSun]
    #22004367 - 07/27/15 12:49 PM (8 years, 6 months ago)

Are you familiar with using agar?


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OfflineMorel Guy
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Re: Cloning question [Re: thebitterbuffalo26]
    #22004417 - 07/27/15 12:58 PM (8 years, 6 months ago)

Probably best to start the spores on agar.  This way you know you have rizamorphic myc.  Start several cultures and do small casings to determine potency and yeild of fruits.  You can clone or you can just have kept a wedge of your original cultures.


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"in sterquiliniis invenitur in stercore invenitur"

In filth it will be found in dung it will be found


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Invisiblemaddchef
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Re: Cloning question [Re: Morel Guy]
    #22004475 - 07/27/15 01:13 PM (8 years, 6 months ago)

Don't listen to this. To clone you simply tear your fruit in half and aseptically take a tissue sample from the core.

You can drop a pin on agar but you will have to transfer a few times likely.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Cloning question [Re: maddchef]
    #22005270 - 07/27/15 04:03 PM (8 years, 6 months ago)

Quote:

maddchef said:
Don't listen to this. To clone you simply tear your fruit in half and aseptically take a tissue sample from the core.




This. RR has a video on it.

Quote:

You can drop a pin on agar but you will have to transfer a few times likely.




You can but I've never liked this method. Pins want to roll around on the agar. If you just take a little wedge of flesh you can put it in the divot that was made in the agar while cooling the scalpel.

I also like to clone from the inside of the cap instead of the stem. It's a smaller target most of the time, but the texture isn't so stringy.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineDocG
Hesco cobra

Registered: 07/09/15
Posts: 28
Last seen: 5 years, 10 months
Re: Cloning question [Re: 36fuckin5]
    #22005792 - 07/27/15 05:43 PM (8 years, 6 months ago)

I am not familiar with using agar. Is it really as simple as dropping the tissue in and letting it do its thing?  I appreciate all of the help!


--------------------
Friendly Reminder

66,000 mph. That is how fast we are hurdling through space.
1,600,000 miles. That is how far we travel every day.
All while spinning at around 1000 mph.


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OfflineDocG
Hesco cobra

Registered: 07/09/15
Posts: 28
Last seen: 5 years, 10 months
Re: Cloning question [Re: DocG]
    #22005850 - 07/27/15 05:51 PM (8 years, 6 months ago)

Also what cultivation tek is best for clones? Sorry if this seems like common sense.


--------------------
Friendly Reminder

66,000 mph. That is how fast we are hurdling through space.
1,600,000 miles. That is how far we travel every day.
All while spinning at around 1000 mph.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
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Re: Cloning question [Re: DocG]
    #22005979 - 07/27/15 06:18 PM (8 years, 6 months ago)

Quote:

DocG said:
Also what cultivation tek is best for clones? Sorry if this seems like common sense.



http://www.shroomery.org/forums/showflat.php/Number/21997518

this is one of the ways I cultivate for the sole purpose of getting a clone.


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OfflineDocG
Hesco cobra

Registered: 07/09/15
Posts: 28
Last seen: 5 years, 10 months
Re: Cloning question [Re: bodhisatta]
    #22006254 - 07/27/15 06:49 PM (8 years, 6 months ago)

Thank you so much that link is super helpful!


--------------------
Friendly Reminder

66,000 mph. That is how fast we are hurdling through space.
1,600,000 miles. That is how far we travel every day.
All while spinning at around 1000 mph.


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OfflineTheMustardTiger
Male


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Posts: 4,700
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Last seen: 6 years, 5 months
Re: Cloning question [Re: Morel Guy]
    #22006300 - 07/27/15 06:56 PM (8 years, 6 months ago)

Quote:

Morel Guy said:
Probably best to start the spores on agar.  This way you know you have rizamorphic myc.  Start several cultures and do small casings to determine potency and yeild of fruits.  You can clone or you can just have kept a wedge of your original cultures.




Bad information and incorrect use of cultivation vocabulary.


--------------------


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InvisibleInocuole
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Re: Cloning question [Re: DocG]
    #22006316 - 07/27/15 06:58 PM (8 years, 6 months ago)

Quote:

DocG said:
I am not familiar with using agar. Is it really as simple as dropping the tissue in and letting it do its thing?  I appreciate all of the help!




It requires sterile technique but yes, it's basically that simple.  Tissue samples from the inside of the base of the fruit/pin are cleanest and most rigorous.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Invisible36fuckin5
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Re: Cloning question [Re: TheMustardTiger]
    #22006808 - 07/27/15 08:46 PM (8 years, 6 months ago)

Quote:

TheMustardTiger said:
Quote:

Morel Guy said:
Probably best to start the spores on agar.  This way you know you have rizamorphic myc.  Start several cultures and do small casings to determine potency and yeild of fruits.  You can clone or you can just have kept a wedge of your original cultures.




Bad information and incorrect use of cultivation vocabulary.




No, his info was right. And nothing was misused. Rhizomorphic is misspelled.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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InvisibleInocuole
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Re: Cloning question [Re: 36fuckin5]
    #22007065 - 07/27/15 09:48 PM (8 years, 6 months ago)

"Do small casings to determine potency"

Casings don't determine potency, they help maintain a microclimate at the sub surface.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Invisiblemaddchef
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Re: Cloning question [Re: Inocuole] * 1
    #22007186 - 07/27/15 10:18 PM (8 years, 6 months ago)

I like turtles.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleInocuole
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Re: Cloning question [Re: maddchef]
    #22007262 - 07/27/15 10:35 PM (8 years, 6 months ago)

Quote:

maddchef said:
I like turtles.




:thatsayes:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Invisiblejbaby007
Badass
I'm a teapot


Registered: 02/28/15
Posts: 1,026
Re: Cloning question [Re: 36fuckin5]
    #22007304 - 07/27/15 10:45 PM (8 years, 6 months ago)

Quote:

36fuckin5 said:
I also like to clone from the inside of the cap instead of the stem. It's a smaller target most of the time, but the texture isn't so stringy.




So simple yet so helpful! Ugh I get so annoyed trying to take tissue from the stem. It's like peeling the top of a celery stalk. :themoreyouknow:


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OfflineKizzle
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Re: Cloning question [Re: jbaby007]
    #22007917 - 07/28/15 03:12 AM (8 years, 6 months ago)

Quote:

You can but I've never liked this method. Pins want to roll around on the agar.



Yeah that's exactly why I don't do that anymore except with sterile agar pins sometimes. You're screwed if it rolls out of place, bacteria colonies popping up everywhere so it's pretty much impossible to isolate any clean mycelium. It only has to occur a few times and then it's "Fuck this!" :lol:


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Invisiblemaddchef
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Re: Cloning question [Re: Kizzle]
    #22008297 - 07/28/15 07:47 AM (8 years, 6 months ago)

To be fair its really easy to make a divot when you cool your scalpel. But indeed, if it rolls its game over.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Cloning question [Re: Inocuole]
    #22008786 - 07/28/15 10:10 AM (8 years, 6 months ago)

Quote:

Inocuole said:
"Do small casings to determine potency"

Casings don't determine potency, they help maintain a microclimate at the sub surface.




A tray can be referred to as a casing. Has been for years.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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InvisibleInocuole
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Re: Cloning question [Re: 36fuckin5]
    #22008788 - 07/28/15 10:11 AM (8 years, 6 months ago)

Quote:

36fuckin5 said:
Quote:

Inocuole said:
"Do small casings to determine potency"

Casings don't determine potency, they help maintain a microclimate at the sub surface.




A tray can be referred to as a casing. Has been for years.




:justnotright:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Invisiblemaddchef
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Re: Cloning question [Re: Inocuole]
    #22008819 - 07/28/15 10:20 AM (8 years, 6 months ago)

I'm going to make up 3 casings and case them with perlite.

Also, I like turtles


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineKizzle
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Re: Cloning question [Re: maddchef]
    #22008909 - 07/28/15 10:39 AM (8 years, 6 months ago)

Quote:

maddchef said:
To be fair its really easy to make a divot when you cool your scalpel. But indeed, if it rolls its game over.



True. I've been working under the assumption though that it's best to minimize the area of the pin that will be touching the agar during that kind of transfer as it minimizes the chance of any mold spores on the pin coming into direct contact with the agar where they'd be able to germinate.


--------------------


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InvisibleInocuole
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Re: Cloning question [Re: maddchef]
    #22009264 - 07/28/15 12:12 PM (8 years, 6 months ago)

Quote:

maddchef said:
I'm going to make up 3 casings and case them with perlite.

Also, I like turtles




:uhno:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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OfflinetaGyo
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Re: Cloning question [Re: Inocuole]
    #22009577 - 07/28/15 12:59 PM (8 years, 6 months ago)

Quote:

Inocuole said:
Quote:

36fuckin5 said:
Quote:

Inocuole said:
"Do small casings to determine potency"

Casings don't determine potency, they help maintain a microclimate at the sub surface.




A tray can be referred to as a casing. Has been for years.




:justnotright:



Quote:

Inocuole said:
Quote:

maddchef said:
I'm going to make up 3 casings and case them with perlite.

Also, I like turtles




:uhno:



:thefuckisthis:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Edited by taGyo (07/28/15 01:00 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Cloning question [Re: Inocuole]
    #22009645 - 07/28/15 01:15 PM (8 years, 6 months ago)

Quote:

Inocuole said:
Quote:

36fuckin5 said:
Quote:

Inocuole said:
"Do small casings to determine potency"

Casings don't determine potency, they help maintain a microclimate at the sub surface.




A tray can be referred to as a casing. Has been for years.




:justnotright:



:whathesaid:


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InvisibleInocuole
Scalpel of Evil's Bane
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Re: Cloning question [Re: bodhisatta]
    #22009809 - 07/28/15 01:51 PM (8 years, 6 months ago)

Yeah.. the frequency of a misnomer doesn't make it any more correct...


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Cloning question [Re: Inocuole]
    #22012597 - 07/28/15 10:52 PM (8 years, 6 months ago)

The beauty of English is how adaptive it is. That's the only beauty of English. The rest is a fucking wreck.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
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