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Mr.Top_Hat
The guy with a hat


Registered: 05/07/12
Posts: 114
Last seen: 3 years, 4 months
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First time working with agar about to transfer have questions.
#21999487 - 07/26/15 11:16 AM (8 years, 6 months ago) |
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Hello everyone,
So finally I was able to get quite a few of my dishes to germinate which I am extremely happy about. Now I believe it is time for a transfer to new dishes but I am not sure. My myc on my dishes seem a bit puffy and not like any of the dishes I have seen in the teks or even RR's videos. It looks like Myc grew like myc but is just puffy and seems to get thicker. I was wondering if that is a sign of contam or if that is the genetics of what I got. I have taken some photos to show you they are the best I can grab due to camera restrictions and the tek I used which is Pasty's no pour tek. I am pretty confident that my myc is fine and just needs transferred but a little reassurance would be nice. I am also wondering if I left them growing too long its been a lil over a week since germination. I have read transferring early is better than late but I am still a bit unsure.





Edited by Mr.Top_Hat (07/26/15 11:21 AM)
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Galba Cubensis
azur's handdoll



Registered: 06/16/15
Posts: 173
Loc: Norway
Last seen: 8 years, 5 months
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Re: First time working with agar about to transfer have questions. [Re: Mr.Top_Hat]
#21999511 - 07/26/15 11:21 AM (8 years, 6 months ago) |
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What kind of agar?
-------------------- RETARDS! I'LL IGNORE ALL OF YOUR ADVICE! BUT TELL ME WHAT TO DO STILL!
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: First time working with agar about to transfer have questions. [Re: Mr.Top_Hat]
#21999513 - 07/26/15 11:21 AM (8 years, 6 months ago) |
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What strain is this?
The last pic is fine, the one that's all over the place I would shoot again. Some strains are like this though. I have an AA+ that only gives me the unconformed growth you see above and a KSSS that gets extremely puffy.
I read it was because of multiple genetics at play.
For the sake of experimentation and learning I would transfer both away and start new plates and germinate again to see if you get different growth. This way if you like that growth more you can transfer and you already have a little experience.
Transferring is tricky at first but easy after your first few times. Keep your hand steady and you'll be golden.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Mr.Top_Hat
The guy with a hat


Registered: 05/07/12
Posts: 114
Last seen: 3 years, 4 months
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Re: First time working with agar about to transfer have questions. [Re: taGyo]
#21999532 - 07/26/15 11:25 AM (8 years, 6 months ago) |
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I used PDA for my agar mix.
They are both cubes. It all looks like myc I see no signs of contam but again I am brand new to agar and was just trying to be sure since I used a year old prints from my last grow. I guess I will mix up another batch and transfer then mix up another batch and try again from my print.
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DeTwizzle
Anyone


Registered: 06/08/14
Posts: 353
Last seen: 5 years, 8 months
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Re: First time working with agar about to transfer have questions. [Re: Mr.Top_Hat]
#21999535 - 07/26/15 11:27 AM (8 years, 6 months ago) |
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I just started with agar too, and that is what my dishes look like off MS syringes. I think all the images that you see of very rhinzomatic growth is from ppl 'sectoring' their initial growths. Basically look for the most rhinzo looking myc on the dish that is also growing the fastest and continue to sector, transfer, and isolate the most vigorous and fastest growing mycelium. I think that may be the reason initial agar work doesn't not look like those amazing dishes ppl post up that are super webby.
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Mr.Top_Hat
The guy with a hat


Registered: 05/07/12
Posts: 114
Last seen: 3 years, 4 months
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Re: First time working with agar about to transfer have questions. [Re: DeTwizzle]
#21999567 - 07/26/15 11:32 AM (8 years, 6 months ago) |
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Quote:
DeTwizzle said: I just started with agar too, and that is what my dishes look like off MS syringes. I think all the images that you see of very rhinzomatic growth is from ppl 'sectoring' their initial growths. Basically look for the most rhinzo looking myc on the dish that is also growing the fastest and continue to sector, transfer, and isolate the most vigorous and fastest growing mycelium. I think that may be the reason initial agar work doesn't not look like those amazing dishes ppl post up that are super webby.
I never expected it to look as good as those nice plates I was worried about puffy myc hoping it wasn't like bacterial contamination.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: First time working with agar about to transfer have questions. [Re: Mr.Top_Hat]
#21999576 - 07/26/15 11:34 AM (8 years, 6 months ago) |
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Here's a recent post on tomentose/rhizo to explain it a bit more. You don't need to transfer to get rhizomorphic:
Quote:
taGyo said:
Quote:
User said: Hopefully, I can find create a good monoculture.

Monos are created after many, many transfers, not found. IMO, start from a clone and work your way to it. At least then you know fruiting potential so if you get less then you know you fucked up or if you get more or the same then you're good. A baseline is always good.
Some of my best grows were tomentose. BRF is highly nutritious for mycelium so it doesn't surprise me that you're seeing tomentose growth. Tomentose is not good, nor is rhizomorphic, it's just a growth pattern. You can get the most rhizo tub in the world and it can be complete shit come fruiting time.
What tomentose myc is just means that your culture is satisfied with it's food source. Rhizomorphic happens when the mycelium has to stretch to find nutrients so it expands faster and reaches longer. Sometimes I have rhizo on agar and then transfer it to grain and it goes tomentose because it's no longer searching for available nutrients. Tomentose, however, typically grows slower because it's fine with the nutrient content.
I can sometimes invoke rhizo by using less agar. Instead of, say, a 1/2 inch of agar I put a 1/4" in my plates and voila, rhizo.
So in truth, is rhizo (Starving myc) better then tomentose?
When growing weed, you don't starve your plant to make it go faster, you let it take it's time.
Worry about health of the culture, not growth pattern, and you'll be on the right track.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Galba Cubensis
azur's handdoll



Registered: 06/16/15
Posts: 173
Loc: Norway
Last seen: 8 years, 5 months
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Re: First time working with agar about to transfer have questions. [Re: DeTwizzle]
#21999577 - 07/26/15 11:34 AM (8 years, 6 months ago) |
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Well wheres the blue tint from, the plastic (lid)?
-------------------- RETARDS! I'LL IGNORE ALL OF YOUR ADVICE! BUT TELL ME WHAT TO DO STILL!
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DeTwizzle
Anyone


Registered: 06/08/14
Posts: 353
Last seen: 5 years, 8 months
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Re: First time working with agar about to transfer have questions. [Re: Mr.Top_Hat]
#21999598 - 07/26/15 11:39 AM (8 years, 6 months ago) |
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Yeah, bacterial looks like snot...have you never seen it in your grain spawn, a pf cake, or a mono? It's that gelatony goo with a yellow to amber tint.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: First time working with agar about to transfer have questions. [Re: DeTwizzle]
#21999611 - 07/26/15 11:42 AM (8 years, 6 months ago) |
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Some common contams:
http://www.shroomery.org/forums/showflat.php/Number/20896027
Quote:
taGyo said: You Know You Dun' Fucked Up. Contaminations
Rye Bacteria, PC Puerto Rico, Came From Bad Spawn:
 
More Bacteria:

Mold, PC Puerto Rico, Improper PC Use:

Filled With Spare BRF, Left For A Month With No GE or Sterilization, Cobweb And Mold:

Premade Jars:

Agar Bacteria:

APE germinating on bacteria:

Aspergilus from dragging a plate through the air:
 
Unidentified Contam from touching agar plate after iso:
 
Black Mold on spare agar, Identified as Aspergilus Niger:

Unknown Contam:

Unknown contam:

Unknown:

Trichoderma:
 
 
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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