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Invisibleeatyualive
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Re: PF tek for cloning on the EZ [Re: MikeBearPig]
    #22021203 - 07/30/15 05:57 PM (8 years, 5 months ago)

at previous living situations all i could get was medium coarse grade verm. so i grew up on pf tek with it. when i moved, they only had fine verm. i actually prefer the coarse for pf jars. i find the jars to colonize faster. but, i have to add more water when i use the medium coarse in my bulk subs.

recently the place i used to get the fine verm at now only carries the medium coarse. so my normal water ratios have had to be increased. if i make 3 tubs worth of subs and add 15 quarts of water. i now had to add 4 more cups of water using the large verm. see you and i are twins bod. we use the same verm.


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Edited by eatyualive (07/30/15 09:15 PM)


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Re: PF tek for cloning on the EZ [Re: eatyualive]
    #22022064 - 07/30/15 09:07 PM (8 years, 5 months ago)

:lol: that's' sort of weird when you consider fine verm should hold more water by volume, but either way it's really no big deal


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Invisibleeatyualive
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Re: PF tek for cloning on the EZ [Re: cronicr]
    #22022094 - 07/30/15 09:14 PM (8 years, 5 months ago)

it is weird. why did i have to add so much extra last night? i mixed in the normal 5 quarts per brick of coir with 2 quarts verm for the cvg mix. even at spawning it seemed a little dry. :tongue2:

now, those coir bricks were a little big this time. that is probably the difference. probably the 3 biggest ive seen in those eco earth 3 packs. ah i meant 4 cups not quarts. i updated it. i added 4 more cups than normal so 1 quart.


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InvisiblebodhisattaMDiscordReddit
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Re: PF tek for cloning on the EZ [Re: bodhisatta]
    #22087837 - 08/13/15 03:38 PM (8 years, 5 months ago)

well everything I used that PESH syringe I thought was clean on ended up fucked. a couple muda jars a couple grain jars and that tall cake from the OP in this thread.


got some knots forming on the shooting star cake though


always forget to un-click that stupid rotation box.


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InvisiblebodhisattaMDiscordReddit
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Re: PF tek for cloning on the EZ [Re: bodhisatta]
    #22098266 - 08/16/15 10:32 AM (8 years, 5 months ago)



Time to do some clones
:uptosomething:


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InvisiblebodhisattaMDiscordReddit
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Re: PF tek for cloning on the EZ [Re: bodhisatta]
    #22098950 - 08/16/15 02:21 PM (8 years, 5 months ago)



:derfase:
Cloning is easy. I'll make a transfer soon after there's growth on the dish so I didn't bother taking very many precautions. I cut the pin off with a sterile scalpel and using cleaned tweasers put it on a dish.


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InvisibleMikeBearPig
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Re: PF tek for cloning on the EZ [Re: bodhisatta]
    #22102884 - 08/17/15 01:42 PM (8 years, 5 months ago)

Quote:

bodhisatta said:


:derfase:
Cloning is easy. I'll make a transfer soon after there's growth on the dish so I didn't bother taking very many precautions. I cut the pin off with a sterile scalpel and using cleaned tweasers put it on a dish.





The thing I don't get is.. The way you did this one.. All it would take is for the air to exchange 1 time in the bag and might as well throw it in a SGFC.  It's not any more sterile than waving it around in the air.

Did you fruit it in front of a flow hood?  If not, then this looks like a method of fruiting, not cloning.


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OfflinePussyFart
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Re: PF tek for cloning on the EZ [Re: MikeBearPig]
    #22102909 - 08/17/15 01:52 PM (8 years, 5 months ago)

Quote:

MikeBearPig said:
Quote:

bodhisatta said:


:derfase:
Cloning is easy. I'll make a transfer soon after there's growth on the dish so I didn't bother taking very many precautions. I cut the pin off with a sterile scalpel and using cleaned tweasers put it on a dish.





The thing I don't get is.. The way you did this one.. All it would take is for the air to exchange 1 time in the bag and might as well throw it in a SGFC.  It's not any more sterile than waving it around in the air.

Did you fruit it in front of a flow hood?  If not, then this looks like a method of fruiting, not cloning.



The young and vigorus mycellium from the pin will most likely outrun any contams on agar.....

The entire point of agar is to clean up the culture, so even if there was a contam, it should be easily remedied.


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OfflinecronicrFacebook
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Re: PF tek for cloning on the EZ [Re: PussyFart]
    #22102962 - 08/17/15 02:17 PM (8 years, 5 months ago)

:whathesaid: i chuck pins fruited from a sgfc/mono/gh on agar all the time:super:


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InvisibleMikeBearPig
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Re: PF tek for cloning on the EZ [Re: PussyFart]
    #22102974 - 08/17/15 02:20 PM (8 years, 5 months ago)

Quote:

PussyFart said:
Quote:

MikeBearPig said:
Quote:

bodhisatta said:


:derfase:
Cloning is easy. I'll make a transfer soon after there's growth on the dish so I didn't bother taking very many precautions. I cut the pin off with a sterile scalpel and using cleaned tweasers put it on a dish.





The thing I don't get is.. The way you did this one.. All it would take is for the air to exchange 1 time in the bag and might as well throw it in a SGFC.  It's not any more sterile than waving it around in the air.

Did you fruit it in front of a flow hood?  If not, then this looks like a method of fruiting, not cloning.



The young and vigorus mycellium from the pin will most likely outrun any contams on agar.....

The entire point of agar is to clean up the culture, so even if there was a contam, it should be easily remedied.




Nobody said otherwise.. What was said is there isn't any different than any other process we use.  The bag or methods used here are no different than using a fruiting chamber and pretending that cutting it with a sterile knife would matter.  Even down to the agar, its all the same.. 

I am trying to grasp why this method that was used here differs from the many other tek's.  If you could provide some actual facts about why this is better and not to cleaning it up to agar, that would be much better and easier for me to understand.


Edit : Maybe I am picking up on some sort of shortage of info here.

I really think you should make sure that people know that the bag's or methods you use are not going to produce fruits any more sterile than growing them next to a pile of shit, or this whole thread.


Edited by MikeBearPig (08/17/15 02:23 PM)


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Offlinespacechildo
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Re: PF tek for cloning on the EZ [Re: MikeBearPig]
    #22102993 - 08/17/15 02:26 PM (8 years, 5 months ago)

its EZ because you dont need a fc you just use a plastic bag.
not many bulk growers still have their sgfc's and this is just an easy way to get a pin.

at least that's how I see it.


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InvisiblebodhisattaMDiscordReddit
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Re: PF tek for cloning on the EZ [Re: spacechildo]
    #22103052 - 08/17/15 02:50 PM (8 years, 5 months ago)

Quote:

spacechildo said:
its EZ because you dont need a fc you just use a plastic bag.
not many bulk growers still have their sgfc's and this is just an easy way to get a pin.

at least that's how I see it.



Yep doesn't take an IQ over 100 to figure out that most ppl don't want a whole SGFC if they want to do a single cake or even a few cakes just to get a Clone. I don't want a 30+qt tub for the couple cakes Im doing.

It never said it was a cloning TEK. It's a pf TEK to skip the big FC to make it more appealing to do PF cakes for clones.

It makes no difference how clean or open air your fruits are for cloning. If you don't cometely suck dick at sterile techniques you can drop the pin right on agar from an open air grow.


Quote:

MikeBearPig said:

The thing I don't get is.. The way you did this one.. All it would take is for the air to exchange 1 time in the bag and might as well throw it in a SGFC.  It's not any more sterile than waving it around in the air.

Did you fruit it in front of a flow hood?  If not, then this looks like a method of fruiting, not cloning.



:smbfacepalm:
you have to read the OP for it to make sense

If you read the OP it says its for fruiting not cloning. It's an appealing way to fruit cakes, skip the big FC. A SGFC just to do test cakes is much too big and overkill.

it's for people who nay say the PF tek since it's soo much hassle, this offers an alternative twist on the PF tek so that it's EZ to use it to get clones. if you already have a SGFC set up then obviously use that. if you are turned away from the idea of using a really simple PF cake to clone because of the SGFC, then think outside the box and use a plastic bag and make the PF tek EZ.

technically I could have called this PF tek for PF tek on the EZ. you could fruit dozens of these on a shelf with a 3 dollar box of bags, you could re-use. and it would be just as simple as the SGFC/PF tek aside from not having the jars available right away



Edited by Trusted cuItivator (08/17/15 03:29 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: PF tek for cloning on the EZ [Re: bodhisatta]
    #22104189 - 08/17/15 07:39 PM (8 years, 5 months ago)



probably just chomp these tomorrow,



this is a great way to test your LC too,


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Re: PF tek for cloning on the EZ [Re: bodhisatta]
    #26079271 - 06/28/19 01:19 PM (4 years, 6 months ago)

Wow, amazing stuff, can't wait!


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Re: PF tek for cloning on the EZ [Re: Secretlabcoat]
    #26079296 - 06/28/19 01:29 PM (4 years, 6 months ago)

Blast from the past. Brings back memories of such asshurt people


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OfflineBentoGrow
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Re: PF tek for cloning on the EZ [Re: bodhisatta]
    #26079396 - 06/28/19 02:40 PM (4 years, 6 months ago)

Awesome tek! I will give it a try on a few jars that I will use. I'm into plastic jars since in this part of the world glass jars are not wide mouth...

Thanks!


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Re: PF tek for cloning on the EZ [Re: bodhisatta]
    #26116712 - 07/18/19 07:15 PM (4 years, 6 months ago)

Would this yield about the same as a SGFC? I have only 3 cakes that should be birthed in a few days and because im already doing agar and look forward to using only monos for bulk i really dont want to have to buy and make a 36l SGFC for only one use. I would still like to get more than a few clones also hehe.

What would you suggest? Do the ziplock backs have any holes made in them?

Thank you for your time and help mate, its bean a pleasure to learn from your info


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InvisiblebodhisattaMDiscordReddit
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Re: PF tek for cloning on the EZ [Re: Sankhara]
    #26116753 - 07/18/19 07:39 PM (4 years, 6 months ago)

Probably a little less honestly but cakes do about 2-7 grams first flush.


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Re: PF tek for cloning on the EZ [Re: bodhisatta]
    #26374420 - 12/11/19 05:10 AM (4 years, 1 month ago)

Great TEK Bod. I have one question though. Would wrapping the jar in foil to prevent light from getting in stop side pinning or is side pinning inevitable with this tek?


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Re: PF tek for cloning on the EZ [Re: Chewzer]
    #26374422 - 12/11/19 05:18 AM (4 years, 1 month ago)

Light doesn't cause side pinning anyway


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