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OfflineTweakz
Professional contam grower


Registered: 07/09/15
Posts: 154
Last seen: 1 month, 27 days
Re: Need help with V-tek, No growth, Worried. [Re: jesuisravi]
    #21997829 - 07/25/15 11:50 PM (8 years, 6 months ago)

Quote:

jesuisravi said:
I hope you are not giving up on the rice jars yet.





nah i'm still hopeful that they will succeed but i just dont want to spend a month waiting to see if it will colonize without having a backup batch.


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OfflineMachiavelliavore
Vermiculite Hater
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Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
Re: Need help with V-tek, No growth, Worried. [Re: Tweakz]
    #21997835 - 07/25/15 11:51 PM (8 years, 6 months ago)

Just make it out of rice.  Grain colonizes a faster than PF, especially with a shake.

If you think you can't get V-tek to work, just hydrate a quart of coir to field capacity, nuke it, and add an equal quantity of it to your grains, then shake the shit out of it.  Should still be enough room to fruit invitro.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (07/26/15 12:27 AM)


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OfflineTweakz
Professional contam grower


Registered: 07/09/15
Posts: 154
Last seen: 1 month, 27 days
Re: Need help with V-tek, No growth, Worried. [Re: Machiavelliavore]
    #21998199 - 07/26/15 01:21 AM (8 years, 6 months ago)

Quote:

Machiavelliavore said:
Just make it out of rice.  Grain colonizes a faster than PF, especially with a shake.

If you think you can't get V-tek to work, just hydrate a quart of coir to field capacity, nuke it, and add an equal quantity of it to your grains, then shake the shit out of it.  Should still be enough room to fruit invitro.




thanks for the tip machiavelli i may do that to the dryer jars soon. just got a fresh bag of peat moss, which from my understanding is basically the same thing.


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OfflineMachiavelliavore
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Re: Need help with V-tek, No growth, Worried. [Re: Tweakz]
    #21998362 - 07/26/15 03:29 AM (8 years, 6 months ago)

Btw, I should clarify, only add coir after full colonization of the grains if you want to use a bulk substrate instead of v-tek watering.  Do not open your jars until full colonization.

Peat has lower nutritional content than coir, it's acidic and it tends not to have a watterlogged texture when hydrated alone.  Coir has some nutritional content (though not much,) hydrates quite nicely to a fluffy consistency, and is more pH balanced.

I have never heard of using peat as a bulk substrate.  If it's possible to do, you probably need to do something like peat + verm 50/50 to get it to a texture the mycellium will like.  It tends not to be colonized as aggressively as coir regardless.  It makes a better casing layer (non-nutritious, high humidity pinning surface) because it seems to have higher water capacity/not dry out easily, while being less nutritious (good for inducing pinning,) and not fully colonizing as easily (good for casing.)  I doubt you want to sacrifice your spiritual quest/lulzfiesta to experimentation that nobody will advise you to do :smile:

Using straight peat as a casing requires a high degree of proficiency in hydrating it correctly, so I would use 50/50 peat verm, and just do v-tek cased invitro containers.

If you wanna do the bulk thing, buy some coir.  It's like $3 at petstores.  You could also use straight vermiculite hydrated to field capacity as a bulk sub, but it would be more expensive and less nutritious than coir.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineTweakz
Professional contam grower


Registered: 07/09/15
Posts: 154
Last seen: 1 month, 27 days
Re: Need help with V-tek, No growth, Worried. [Re: Machiavelliavore]
    #21998414 - 07/26/15 04:36 AM (8 years, 6 months ago)

oh okay i think i got it mixed up, peat moss seems to be just for casing.


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OfflineMachiavelliavore
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Re: Need help with V-tek, No growth, Worried. [Re: Tweakz]
    #21998420 - 07/26/15 04:40 AM (8 years, 6 months ago)

They do have equivalent uses in horticulture and in casing layers, so you were right about that.  Coir just also makes a good bulk substrate while peat afaik does not.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleViolet
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Registered: 12/06/11
Posts: 4,205
Re: Need help with V-tek, No growth, Worried. [Re: Machiavelliavore]
    #22000960 - 07/26/15 05:39 PM (8 years, 6 months ago)

Tweaks, you don't really appear to be following the tek of mine you linked.
Firstly, brown rice is not given as an intended substrate for the tek – only an alternative for growers when they get to the point of being better at grain preparations and are ready to use casing layers.
Secondly, you're using a jar, which wouldn't be a problem if you had an unmodified plastic lid (as that would technically be following the tek),
but having so little substrate in a jar (yours sub is especially small btw) that large can have moisture problems when the grain isn't thoroughly hydrated and the lid allows moisture loss like yours definitely does thru the poly-fil.  It's pretty clear from the sides of the jar that you're low on moisture.  It may still grow, but...
Thirdly, dropping dry spores onto the top of grain like that is something meant for the likes of my culturing tek, and that ONLY, in an entirely-enclosed container with plenty of moisture.  Between those two things, it's possible that you might have trouble getting quick growth, even growth at all, from the tops of the sub which is the most prone to drying out, and almost the only places your spores likely landed.

It's possible you just haven't had germination yet but may yet.  But personally I would set that grow aside and start something else.  If you get growth on those jars, just use them to spread some mycelium to another grow if you don't have another source of mycelium first.

In the future, keep in mind it's best to have your spores hydrated and in a moisture-retaining environment for germination. Such as on agar, in a spore solution, or like is possible with my culture tek by putting them on well-hydrated grain in a smaller and enclosed container.

If you want to use methods like mine as a beginner using multi-spore inoculations without agar, having your spores in solution syringes is fairly important.  As is using a solid amount of substrate in the invitro containers - 1/4 full!
Try following the instructions outlined in teks to-a-T.
And Im happy to help out!


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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OfflineTweakz
Professional contam grower


Registered: 07/09/15
Posts: 154
Last seen: 1 month, 27 days
Re: Need help with V-tek, No growth, Worried. [Re: Violet]
    #22002152 - 07/26/15 09:52 PM (8 years, 6 months ago)

hmm so it seems this  may not work. Luckily yesterday i prepared some pf tek jars as a backup. Would taping the polyfill lids be benificial at this stage?

i'll try your tek again when i can find those plastic containers and grass seed.


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OfflineTweakz
Professional contam grower


Registered: 07/09/15
Posts: 154
Last seen: 1 month, 27 days
Re: Need help with V-tek, No growth, Worried. [Re: Tweakz]
    #22018105 - 07/30/15 01:28 AM (8 years, 5 months ago)

Update:

10 days now no signs of growth, not even a little bit of contam. :sad:
Which means my sterile technique is effective, but i'm starting to think my spores might be dead or something.

pf jars are on day 4 now, hopefully those start colonizing soon. This uncertainty is driving me crazy, every day im thinking "maybe i should make more jars with a different tek and different substrate".

if i saw some colonization i would be able to relax and just wait it out but when you don't know if it will ever colonize it can be stressful.

i feel im alone in this situation too because i constantly see people posting their first ever grow and the pics look amazing.


Edited by Tweakz (07/30/15 01:31 AM)


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