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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Why Slants: Wouldn't a Popsicle stick in a petri work?
#21995623 - 07/25/15 03:01 PM (8 years, 6 months ago) |
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I'm wondering why a slant is advantageous. Are they going for a long skinny stretch of mycelium? Because it seems like a petri has a larger surface area. I could see it being advantageous for the mycelium to have a long skinny substrate so that it takes a long time to eat but doesn't cause a lot of cell division. (senescence, whatever)
Maybe wood in a petri would at least be worth an experiment. Thoughts?
(side note, RR logged in for the first time in over 5 months today, hope he's doing well)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Psilosopherr]
#21996085 - 07/25/15 04:26 PM (8 years, 6 months ago) |
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slants don't dry out nearly as fast due to the surface area aspect
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: cronicr]
#21996136 - 07/25/15 04:36 PM (8 years, 6 months ago) |
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perhaps a deep petri. I'm using tiny jars so I can probly go deeper than a traditional petri.
A deep petri with a wet water/agar ration perhaps.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Psilosopherr]
#21996157 - 07/25/15 04:42 PM (8 years, 6 months ago) |
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well a deep dish will last longer, probably not as long as a slant but i'm sure somebody has a better answer then i
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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stevo


Registered: 04/11/05
Posts: 5,100
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? *DELETED* [Re: Psilosopherr]
#21996179 - 07/25/15 04:48 PM (8 years, 6 months ago) |
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Post deleted by stevo
Reason for deletion: .
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: stevo]
#21996184 - 07/25/15 04:49 PM (8 years, 6 months ago) |
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i'd think grain jars would be an ideal way to store cultures
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: cronicr]
#21996220 - 07/25/15 04:59 PM (8 years, 6 months ago) |
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Hey cron, sup?
You don't use as much agar for slants and deep agar petri dishes will never be deep enough for long term storage, they will end up drying out. Slants can last 10 years or more.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Mr. Alien]
#21996390 - 07/25/15 05:36 PM (8 years, 6 months ago) |
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I've never heard of slants lasting 10 years. Like 1-2 years is what I usually hear.
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nobody83
stranger danger


Registered: 03/15/14
Posts: 1,486
Loc: around town
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Inocuole]
#21996439 - 07/25/15 05:48 PM (8 years, 6 months ago) |
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Quote:
Inocuole said: I've never heard of slants lasting 10 years. Like 1-2 years is what I usually hear.
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Inocuole]
#21996442 - 07/25/15 05:48 PM (8 years, 6 months ago) |
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I did heard of slants lasting 10 years. I heard from eatualive reviving a 10 years old culture and he doesn't even used slants at that time.
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nobody83
stranger danger


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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Mr. Alien] 1
#21996448 - 07/25/15 05:49 PM (8 years, 6 months ago) |
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Eatualive can revive a dead snake
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: nobody83]
#21996466 - 07/25/15 05:53 PM (8 years, 6 months ago) |
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why wouldn't a slant dry? Because its sealed better than a petri? So are my petri jars.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Psilosopherr]
#21996490 - 07/25/15 05:57 PM (8 years, 6 months ago) |
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Surface area. if 10ml of agar has 2 square inches of surface area, that's going to take significantly longer to dry out than 10ml of agar with 5 or 6 square inches of surface area.
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nobody83
stranger danger


Registered: 03/15/14
Posts: 1,486
Loc: around town
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Inocuole]
#21996516 - 07/25/15 06:01 PM (8 years, 6 months ago) |
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Bill nye would approve
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Inocuole]
#21996741 - 07/25/15 06:55 PM (8 years, 6 months ago) |
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Quote:
Inocuole said: Surface area. if 10ml of agar has 2 square inches of surface area, that's going to take significantly longer to dry out than 10ml of agar with 5 or 6 square inches of surface area.

thanks for clarifying all the reasoning everybody.
Especially cron
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: nobody83]
#21996846 - 07/25/15 07:18 PM (8 years, 6 months ago) |
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Quote:
nobody83 said: Eatualive can revive a dead snake
lol.
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Inocuole]
#21996879 - 07/25/15 07:25 PM (8 years, 6 months ago) |
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Quote:
Inocuole said: I've never heard of slants lasting 10 years. Like 1-2 years is what I usually hear.
"(Note that a square centimeter of mycelium is generally transferred from one culture dish to the next.) I have strains in my possession, from which I regularly regenerate cultures, that are 10 years old, and keep at a P2 or P3" Paul stamets.
That's were i heard of that. By regenerating cultures of course... But even then... i think is possible to revive a 10 year old culture slant.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Mr. Alien]
#21996910 - 07/25/15 07:31 PM (8 years, 6 months ago) |
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slants last 10 years stored at -40C
petri dishes unlike slants also have edges the mycelium grows to and can pick up contamination. a tube has the mycelium crawl up the glass a bit but it's not nearly as much of a concern for LTS
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: bodhisatta]
#21997246 - 07/25/15 09:02 PM (8 years, 6 months ago) |
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Quote:
bodhisatta said: slants last 10 years stored at -40C
petri dishes unlike slants also have edges the mycelium grows to and can pick up contamination. a tube has the mycelium crawl up the glass a bit but it's not nearly as much of a concern for LTS
-40C? Or -40F? -40ºC is a lot.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Why Slants: Wouldn't a Popsicle stick in a petri work? [Re: Mr. Alien]
#21997276 - 07/25/15 09:07 PM (8 years, 6 months ago) |
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yes -40c is a lot but yeast libraries use that cold
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