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HairSnake
Science Nerd



Registered: 06/19/15
Posts: 11
Loc: Midwest USA
Last seen: 8 years, 4 months
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Lions Mane experimenting. Start over?
#21995285 - 07/25/15 01:31 PM (8 years, 6 months ago) |
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My success with oyster mushrooms may have had my confidence a bit to high or my expectations unreasonable when I started a lions mane project.
My first thought was to create a back up of the liquid culture so I don't have to buy when I eff it up through inexperience.
1. ) I used a purchased LC syringe and put a few drops into several test tube slants, they grew and actually started to produce primordia before putting them in the fridge.
2. ) I put a few drops into sterilized jars filled with 200cc water and various sugar sources. I am not 100% sure what a successful LC is supposed to looks like but they accumulating "stuff" looks similar to the stuff floating around in syringe. So, I assume it's mycelium. The jar with honey produced the most snotty looking stuff, the Jar with Dex has about half that amount, the jars with brown sugar and turbinado did nothing and remain clear. I think I am OK as for a Back-up with the other two.
3.) The bulk of the syringe solution was used to inoculate 5 PF tek jars. (I sprinkled in some hydrated pine sawdust before realizing that hardwood is what's needed.) Compared to the oyster jars, The mycelium is very thin and almost invisible unless very close. These very thin spider web looking filaments made it all the way to the bottom of the jar 360 degrees over the next 2 weeks. This last week, I finally noticed some 2 to 3 mm spots where there was observable white patches.
The oysters colonized pretty densely from the start and radiated out densely. There was a clear demarcation line between colonized and un-colonized substrate. even then, it happened fairly quickly.
On the test tube slants, there was reasonably dense mycelium from the inoculation point from the start on PDAY.
What the heck is going on in these Jars? Is this typical Lions Mane behavior? Could the pine sawdust be causing problems? Should I abourt and try again with some of the LC I made?
Or is this normal and I need to learn to exercise more patience??
-------------------- A sure sign of happiness is when its difficult to determine whether one is working or playing.
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AIRDOG



Registered: 10/16/99
Posts: 3,493
Loc: world's shroom capital
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Re: Lions Mane experimenting. Start over? [Re: HairSnake]
#21995310 - 07/25/15 01:38 PM (8 years, 6 months ago) |
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hericium mycelium is very wispy and thin until it reaches full colonization then it thickens nicely
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Jenn
Supreme Leader



Registered: 04/27/15
Posts: 200
Last seen: 7 years, 11 months
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Re: Lions Mane experimenting. Start over? [Re: AIRDOG]
#21996148 - 07/25/15 04:40 PM (8 years, 6 months ago) |
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Quote:
AIRDOG said: hericium mycelium is very wispy and thin until it reaches full colonization then it thickens nicely
Yep. Found this out too a few months ago. But, yeah, AIRDOG is right.
-------------------- Sit down before fact as a little child, be prepared to give up every preconceived notion, follow humbly wherever and to whatever abysses nature leads, or you shall learn nothing.
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HairSnake
Science Nerd



Registered: 06/19/15
Posts: 11
Loc: Midwest USA
Last seen: 8 years, 4 months
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Re: Lions Mane experimenting. Start over? [Re: Jenn]
#21998511 - 07/26/15 06:04 AM (8 years, 6 months ago) |
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http://
So, do you think this looks ok for 3 weeks after inoculation?
-------------------- A sure sign of happiness is when its difficult to determine whether one is working or playing.
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Alkaloids
3,4,5-trimethoxyphenethylamine



Registered: 11/15/98
Posts: 743
Loc: pubis mons
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Re: Lions Mane experimenting. Start over? [Re: HairSnake]
#22001778 - 07/26/15 08:37 PM (8 years, 6 months ago) |
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That does seem a bit on the slow side for a mycelial syringe. Pine sawdust? That might be causing some slow growth.
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AIRDOG



Registered: 10/16/99
Posts: 3,493
Loc: world's shroom capital
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Re: Lions Mane experimenting. Start over? [Re: Alkaloids]
#22004787 - 07/27/15 02:20 PM (8 years, 6 months ago) |
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yeah it must be the pine... hericium abietis grows well on pine as they have said
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