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Invisible0000000000000
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H2O2, KMNO4 decomp low temp evap (dehumidifier). CHITINASE TEMP STABIULITY, tomatoes, hybridization
    #21993679 - 07/25/15 04:18 AM (8 years, 6 months ago)

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i read CHITINASE helps for hydridization as an alternate to rattlesnake venom.

assuming pH is neutralized from tomatoe acidity (https://en.wikipedia.org/wiki/Chitinase Chitinase occurs naturally in many common foods. This is at least one cause of the cross-reaction phenomenon in latex-fruit syndrome. Bananas, chestnuts, kiwis, avocados, papaya, and tomatoes, for example, all contain significant levels of chitinase.),

what is the stable temperature range for the enzyme.

will Pressure Cooking destroy it?


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InvisibleMad Season
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Re: H2O2, KMNO4 decomp low temp evap (dehumidifier). CHITINASE TEMP STABIULITY, tomatoes, hybridization [Re: 0000000000000]
    #21993688 - 07/25/15 04:26 AM (8 years, 6 months ago)

Quote:

H2O2, KMNO4 decomp low temp evap (dehumidifier). CHITINASE TEMP STABIULITY, tomatoes, hybridization



:notsureif:

Overall after reading your post on contamination forum, and this forum, I'd stay clear from h2o2. It also degrades in the pc. Not really sure why you want/need h2o2. Not sure if either snake venom or whatever your alternative is can survive the pc. If you're working on trying to work on germination/bacterial stuff,  look up the agar sandwich, as well as stros forced hydration


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Edited by Mad Season (07/25/15 04:32 AM)


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Offlinerxb
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Re: H2O2, KMNO4 decomp low temp evap (dehumidifier). CHITINASE TEMP STABIULITY, tomatoes, hybridization [Re: Mad Season]
    #21993799 - 07/25/15 06:01 AM (8 years, 6 months ago)

there are some uses for h2o2, but its not going to survive the pressure cooker.

it degrades in light and heat.

also, the stuff from the pharmacy has things in it which are bad for fungi

gotta get food grade


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. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

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InvisiblebodhisattaMDiscordReddit
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Re: H2O2, KMNO4 decomp low temp evap (dehumidifier). CHITINASE TEMP STABIULITY, tomatoes, hybridization [Re: rxb]
    #21994186 - 07/25/15 09:03 AM (8 years, 6 months ago)

People who can't grow a single shroomery want to talk about hybridization. Fucking college kids


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OfflinetaGyo
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Re: H2O2, KMNO4 decomp low temp evap (dehumidifier). CHITINASE TEMP STABIULITY, tomatoes, hybridization [Re: bodhisatta]
    #21994407 - 07/25/15 09:41 AM (8 years, 6 months ago)

Blackdust...?


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Offlinerxb
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Re: H2O2, KMNO4 decomp low temp evap (dehumidifier). CHITINASE TEMP STABIULITY, tomatoes, hybridization [Re: bodhisatta]
    #21994779 - 07/25/15 11:24 AM (8 years, 6 months ago)

i would love to get started on hybrids at some point.

it seems like there's an endless amount of things to do first :smile:


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->$10 FLOW HOOD ALTERNATIVE <-

. i cleaned a mold contaminated live culture and saved it. (might have useful applications)

[quote]Enlil said:
I'd be the guy with thousands of minions doing my bidding and all of the hot women locked in a cage for my use.[/quote]


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Re: H2O2, KMNO4 decomp low temp evap (dehumidifier). CHITINASE TEMP STABIULITY, tomatoes, hybridization [Re: bodhisatta]
    #21994947 - 07/25/15 12:08 PM (8 years, 6 months ago)

Quote:

bodhisatta said:
People who can't grow a single shroomery want to talk about hybridization. Fucking college kids





I grew a shroomery once.


Op if you're really interested in hybridization there are other ways to go about it aside from weakening and/or destroying cell wass.


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InvisiblePastywhyteMDiscord
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Re: H2O2, KMNO4 decomp low temp evap (dehumidifier). CHITINASE TEMP STABIULITY, tomatoes, hybridization [Re: maddchef]
    #21995534 - 07/25/15 02:40 PM (8 years, 6 months ago)

Yeah hybrids require patience and preparation. Venoms and serial dilution is effective of course but that's really the easy part anyway.  Stabilization is the hard part and that takes time amd generations.


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Invisible0000000000000
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Re: H2O2, KMNO4 decomp low temp evap (dehumidifier). CHITINASE TEMP STABIULITY, tomatoes, hybridization [Re: Pastywhyte]
    #22129570 - 08/23/15 04:00 AM (8 years, 5 months ago)

mad season thanks those two teks are great to know

maddchef, that sounds awesome, more please

idea was to decontam without heat destroying chitinase and/or the H2O2 oxidizing/decomposing the chitinase.

i just read mold makes chitinase. perhaps let it take over, destroy it and use the chitinase, if it is left intact, to help hybridize.

please let me know about hybridization without cell wall weakening and about stabilizing, at least in general.


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