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Isaiahkiki
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Registered: 10/16/14
Posts: 178
Last seen: 3 years, 7 months
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Good isolating a clone write up?
#21991572 - 07/24/15 06:02 PM (8 years, 6 months ago) |
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I've been trying to look for a tek or a write up or something similar on how to go about isolating clones on agar. any help/links would be appreciated
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Morel Guy
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Registered: 01/23/13
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Re: Good isolating a clone write up? [Re: Isaiahkiki]
#21991639 - 07/24/15 06:15 PM (8 years, 6 months ago) |
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I am sure a search will help you out. All I know is the cut comes from inside the stem.
-------------------- "in sterquiliniis invenitur in stercore invenitur" In filth it will be found in dung it will be found
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kmetric



Registered: 08/23/14
Posts: 140
Last seen: 7 years, 10 months
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Re: Good isolating a clone write up? [Re: Morel Guy]
#21992426 - 07/24/15 08:42 PM (8 years, 6 months ago) |
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Don't think this belongs here..but, take a clone then keep isolating on agar until you achieve a monoculture. Morel is right, run a search for monoculture, cloning, agar work.
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: Good isolating a clone write up? [Re: kmetric]
#21992452 - 07/24/15 08:46 PM (8 years, 6 months ago) |
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-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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Isaiahkiki
Stranger


Registered: 10/16/14
Posts: 178
Last seen: 3 years, 7 months
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Re: Good isolating a clone write up? [Re: Toadstool5]
#21995522 - 07/25/15 02:37 PM (8 years, 6 months ago) |
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what rule did i break? my head is so bad you have no idea >.<
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: Good isolating a clone write up? [Re: Isaiahkiki]
#21995604 - 07/25/15 02:56 PM (8 years, 6 months ago) |
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Quote:
2) This forum is for the discussion of advanced techniques and experimental procedures. This forum is NOT a support forum, for problems with your grows. Any posts regarding how to use a pressure cooker, your PF Cakes, a species which has been cultivated ad nauseam, grain ratios, contaminated grows, how to prepare dung, or anything else which has been covered in the forum, etc... will be moved immediately upon review - so please, do not waste the resources, time, or bandwidth of the shroomery.
3) Use the SEARCH feature. This is one of the most comprehensive methods of finding if something has been posted, please use a little bit of creativity in your searches, and append all words which you think you may be able to find posts regarding your subject. It's a very simple and helpful tool. The search engine GOOGLE also has a lot of information.
Its basically not advanced mycology. If you want to discuss the morphology, metabolism, or taxonomy of the mycota kingdom this is the place. If you need help with your grow and want to improve your technique you should ask questions in mushroom cultivation, the noob forum, or gourmet and medicinal.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Good isolating a clone write up? [Re: Toadstool5]
#21997610 - 07/25/15 10:34 PM (8 years, 6 months ago) |
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Is there a way to put at least a post count or member for x amount of time here?
It may sadly keep out someone with good info or a great question but these threads happen more often lately
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: Good isolating a clone write up? [Re: maddchef]
#21997671 - 07/25/15 10:58 PM (8 years, 6 months ago) |
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Yeah its happening a lot, but i think the mods would have to make the change 
Maybe 50 posts in mushroom cult or gourmet/med before gaining access?
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Good isolating a clone write up? [Re: Toadstool5]
#22002799 - 07/27/15 03:32 AM (8 years, 6 months ago) |
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It is a quiet forum anyway so why does it matter? Eventually somebody checks in and sweeps it out but it would be easier to simply pm a mod rathher then post about it lol
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 6 years, 5 months
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Re: Good isolating a clone write up? [Re: cronicr]
#22004730 - 07/27/15 02:14 PM (8 years, 6 months ago) |
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you want to isolate a clone? isn't a clone already a single culture?
-------------------- My trade list In search of sporeless oyster cultures
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Good isolating a clone write up? [Re: knomadic_niki]
#22004836 - 07/27/15 02:30 PM (8 years, 6 months ago) |
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No. It's still random genetics
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 6 years, 5 months
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Re: Good isolating a clone write up? [Re: maddchef]
#22004959 - 07/27/15 02:50 PM (8 years, 6 months ago) |
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i guess you're not talking about a cloned fruit. what is being cloned? does this refer to a transfer from ms culture on agar?
-------------------- My trade list In search of sporeless oyster cultures
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MagicInMichigan
Internal conquistador



Registered: 07/07/15
Posts: 138
Loc: Michigan,U.S.A.
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Re: Good isolating a clone write up? [Re: knomadic_niki]
#22007177 - 07/27/15 10:15 PM (8 years, 6 months ago) |
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I think the OP is looking more along the lines of isolating a sector from MS on agar. The best way I've been told how to do this is to take a very tiny piece from the end of the longest/fastest spread growth area. The smaller/thinner the piece from the end the better chance you have of getting a less varies set of genetics, or at least that's how it was explained to me.
In terms of cloning for certain traits it's much easier to just clone a fruit as opposed to growing out multiple sectors over and over until you have some to test. Doing small scale V-Tek or mudabottle Tek is a good way to do fast& easy grows to see what traits each isolate is expressing and if it's worth cloning the fruits produced based of cluster/size/potency/speed and such.
-MIM
-------------------- “the only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.” I surround myself with people who aren't afraid to live. They have had what they love most taken from them, be it freedom, love, money, or anything for that matter. When you lose your greatest love you also lose your greatest fear, when that happens you are completely and utterly free.
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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 Yeah always clone the tips of the mycellial fans if possible, dont want it to have to re-establish apical dominance unless needed.
Smaller areas do help prevent taking 3 or 4 different intertwined fans but even if you get several different morphologies on the next agar you simply isolate again and again until a monoculture is finally formed. More than likely you will need to transfer at least twice to get a true isolate.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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knomadic_niki
A mile high



Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 6 years, 5 months
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Re: Good isolating a clone write up? [Re: Toadstool5]
#22008658 - 07/28/15 09:40 AM (8 years, 6 months ago) |
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i see, thanks for explaining
-------------------- My trade list In search of sporeless oyster cultures
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MagicInMichigan
Internal conquistador



Registered: 07/07/15
Posts: 138
Loc: Michigan,U.S.A.
Last seen: 8 years, 2 months
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Re: Good isolating a clone write up? [Re: knomadic_niki]
#22008959 - 07/28/15 10:51 AM (8 years, 6 months ago) |
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Quote:
knomadic_niki said: i see, thanks for explaining 
No problem, I just started doing stuff with agar like 3 weeks ago and it was the best thing I think I could have done. Just started my first set of grains using a super aggressive isolate from a Chilean print I had & transferred 3 times, now I just have to wait for them to colonize and fruit so I can clone a giant cluster and then I'll be happy.
-MIM
-------------------- “the only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.” I surround myself with people who aren't afraid to live. They have had what they love most taken from them, be it freedom, love, money, or anything for that matter. When you lose your greatest love you also lose your greatest fear, when that happens you are completely and utterly free.
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Isaiahkiki
Stranger


Registered: 10/16/14
Posts: 178
Last seen: 3 years, 7 months
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I posted here because I read somewhere that if you have any strain isolation or anything with agar it's for advanced growers so my mind put 2 and 2 together. Advanced growing tequnices = advanced mycology. And thanks for the replies I'll be trying to do this shit later this week with ms then when I grow out my mushrooms I'll take a clone I think.
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cronicr


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Good isolating a clone write up? (moved) [Re: Isaiahkiki]
#22048407 - 08/05/15 04:34 PM (8 years, 5 months ago) |
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This thread was moved from Advanced Mycology.
Reason:
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