Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate, Injection Grain Bag   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleMescalitoe
Psychonaut
Male


Registered: 05/14/14
Posts: 584
Loc: California!
Double sterilization?
    #21988681 - 07/24/15 06:54 AM (8 years, 6 months ago)

Hey guys, I'm hoping someone might have the answer to this question.

On Wednesday I made about 12 jars (PF tek). I steam sterilized for about 2 hours and I let them sit overnight to cool. I was supposed to inoculate yesterday but had work from 5 to 5 so when I got home I knocked out. It's Friday morning now. My question is, do you think I should sterilize my jars again or am I good to inoculate? Will sterilizing again burn the brf?


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Double sterilization? [Re: Mescalitoe]
    #21988691 - 07/24/15 07:02 AM (8 years, 6 months ago)

Sterilizing the substrate twice will not burn the brf.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
InvisibleMescalitoe
Psychonaut
Male


Registered: 05/14/14
Posts: 584
Loc: California!
Re: Double sterilization? [Re: hamloaf]
    #21988761 - 07/24/15 07:38 AM (8 years, 6 months ago)

Quote:

hamloaf said:
Sterilizing the substrate twice will not burn the brf.



Fersure man, thanks for the reply.


Extras: Filter Print Post Top
Offlineblackout
 User Gallery

Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 24 days
Trusted Cultivator
Re: Double sterilization? [Re: Mescalitoe]
    #21988853 - 07/24/15 08:22 AM (8 years, 6 months ago)

I wouldn't bother resterilizing them as its only been a few days. In future you can leave them in the fridge if you think its going to be any time.

People worry about moisture loss too. You can weigh your jars when they have cooled and write it on them with permanent marker, this way you can monitor moisture loss at any time.


Extras: Filter Print Post Top
Offlinemotherchimp
Enthusiast


Registered: 03/29/14
Posts: 462
Loc: Australia Flag
Last seen: 8 months, 16 days
Re: Double sterilization? [Re: Mescalitoe]
    #21988857 - 07/24/15 08:24 AM (8 years, 6 months ago)

No need to sterilise them again, I've had sterilised jars sitting for weeks and they are still fine.  If you do choose to re sterilise you can expect the moisture content to be thrown off.


Extras: Filter Print Post Top
OfflineGreenRabbit
Plutonium Pollinator
Male


Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
Re: Double sterilization? [Re: motherchimp]
    #21989025 - 07/24/15 09:13 AM (8 years, 6 months ago)

:whathesaid: Contaminants usually come from the syringe, so leaving them for long periods of time is fine. Theoretically, you could leave them for months or even years as long as they were truly sterilized, but you could loose the proper moisture content by letting them sit for so long. The fridge is best for storing sterilized jars or mycelium you want to slow down.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate, Injection Grain Bag   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* how long can i wait on sterilized jars? Spoogy 1,324 4 08/09/01 10:15 AM
by jonnyshaggs420
* fractional sterilization with BRF Buchu 1,103 3 03/03/04 09:12 PM
by Magash
* sterilizing without a PC
( 1 2 all )
Anonymous 3,015 20 05/28/15 07:01 AM
by Al Bundy
* Need advice on sterility & trading spores. The_Gnome_King 4,359 16 06/25/20 05:22 AM
by Zakkery
* Every how often do i have to sterilize the misting water? Sterile 2,761 8 10/24/23 12:30 PM
by 10ftTall
* About Sterility... bruisedBlue 832 1 08/26/02 03:00 PM
by Hippie3
* Resterilization of substrate? Innernaut 952 5 10/16/03 03:29 PM
by Innernaut
* 2 month old Sterilized PF tek Jars... Please help
( 1 2 all )
Yeti_009 3,812 28 09/26/18 06:08 PM
by Grimsweeper

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,593 topic views. 37 members, 292 guests and 54 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.006 seconds on 12 queries.