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uninc4life2010
Unincorporated



Registered: 06/05/10
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When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB?
#21986798 - 07/23/15 07:19 PM (8 years, 6 months ago) |
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Since my agar dishes come sterilized in a sleeve, I assume that it is generally best to pour all 20 at the same time. My question is: Is it best to tape with parafilm after I pour them in the SAB? I assume that I would need to let them cool off for a few hours first, since I could easily spill them trying to tape while they are still liquid. I feel like I would need to tape them with parafilm since I don't plan on inoculating all 20 at once. Plus, I would want the area around where the top and bottom of the petri come into contact to be taped to prevent contaminants from entering the dish. I maybe plan on doing 3 petris first, and storing the rest in a clean ziploc until I perform transfers/clones or what not. My last concern is getting the parafilm off once the petri is back in the glovebox. I'm just afraid that it might be a little difficult and that I could increase the risk of contamination by fumbling around with it like a noob
Anyways, I'm a little confused about this since I don't yet have experience with agar, so if you all could lend some advice, I would really appreciate the help. Thanks!
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB? [Re: uninc4life2010] 1
#21986819 - 07/23/15 07:22 PM (8 years, 6 months ago) |
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when i used SAB, i just left plates in there, sometimes even a few days, and then put them in gallon zip lock. 20 plates fit in a zip lock easy. i never wrap them before i use them, but u can if u want.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB? [Re: uninc4life2010]
#21986822 - 07/23/15 07:22 PM (8 years, 6 months ago) |
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Nope.
Only use parafilm when the cultures are in there . Before that just put them back in the sleeve.
Or
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
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Re: When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB? [Re: taGyo]
#21986946 - 07/23/15 07:50 PM (8 years, 6 months ago) |
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Quote:
taGyo said: Nope.
Only use parafilm when the cultures are in there . Before that just put them back in the sleeve.
Or 
Okay. They don't run the risk of contamination they aren't sealed? Is it best to put the sleeve in the fridge until they are needed?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB? [Re: blindingleaf]
#21987016 - 07/23/15 08:03 PM (8 years, 6 months ago) |
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Quote:
blindingleaf said: when i used SAB, i just left plates in there, sometimes even a few days, and then put them in gallon zip lock. 20 plates fit in a zip lock easy. i never wrap them before i use them, but u can if u want.
Check out what this guy said.
Nope, I even leave them in my fridge and tape them together in a 4 stack with two strips of long tape and never had a contam problem. I wouldn't advise this for long term storage but you're good bro
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
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Re: When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB? [Re: taGyo]
#21987087 - 07/23/15 08:14 PM (8 years, 6 months ago) |
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The tape actually sounds like a good idea. Thanks!
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