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Shop: Kraken Kratom Red Vein Kratom   North Spore Cultivation Supplies, Injection Grain Bag

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Offlineuninc4life2010
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When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB?
    #21986798 - 07/23/15 07:19 PM (8 years, 6 months ago)

Since my agar dishes come sterilized in a sleeve, I assume that it is generally best to pour all 20 at the same time.  My question is: Is it best to tape with parafilm after I pour them in the SAB?  I assume that I would need to let them cool off for a few hours first, since I could easily spill them trying to tape while they are still liquid.  I feel like I would need to tape them with parafilm since I don't plan on inoculating all 20 at once.  Plus, I would want the area around where the top and bottom of the petri come into contact to be taped to prevent contaminants from entering the dish.  I maybe plan on doing 3 petris first, and storing the rest in a clean ziploc until I perform transfers/clones or what not.  My last concern is getting the parafilm off once the petri is back in the glovebox.  I'm just afraid that it might be a little difficult and that I could increase the risk of contamination by fumbling around with it like a noob  :facepalm3:

Anyways, I'm a little confused about this since I don't yet have experience with agar, so if you all could lend some advice, I would really appreciate the help.  Thanks!  :sunny:


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Invisibleblindingleaf
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Re: When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB? [Re: uninc4life2010] * 1
    #21986819 - 07/23/15 07:22 PM (8 years, 6 months ago)

when i used SAB, i just left plates in there, sometimes even a few days, and then put them in gallon zip lock.  20 plates fit in a zip lock easy. i never wrap them before i use them, but u can if u want.


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OfflinetaGyo
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Re: When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB? [Re: uninc4life2010]
    #21986822 - 07/23/15 07:22 PM (8 years, 6 months ago)

Nope.

Only use parafilm when the cultures are in there :wink:. Before that just put them back in the sleeve.

Or :whathesaid:


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Offlineuninc4life2010
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Re: When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB? [Re: taGyo]
    #21986946 - 07/23/15 07:50 PM (8 years, 6 months ago)

Quote:

taGyo said:
Nope.

Only use parafilm when the cultures are in there :wink:. Before that just put them back in the sleeve.

Or :whathesaid:




Okay.  They don't run the risk of contamination they aren't sealed?  Is it best to put the sleeve in the fridge until they are needed?


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OfflinetaGyo
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Re: When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB? [Re: blindingleaf]
    #21987016 - 07/23/15 08:03 PM (8 years, 6 months ago)

Quote:

blindingleaf said:
when i used SAB, i just left plates in there, sometimes even a few days, and then put them in gallon zip lock.  20 plates fit in a zip lock easy. i never wrap them before i use them, but u can if u want.



Check out what this guy said.

Nope, I even leave them in my fridge and tape them together in a 4 stack with two strips of long tape and never had a contam problem. I wouldn't advise this for long term storage but you're good bro :thumbup:


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Offlineuninc4life2010
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Re: When I pour my agar dishes, do I need to tape them with parafilm while they are still in the SAB? [Re: taGyo]
    #21987087 - 07/23/15 08:14 PM (8 years, 6 months ago)

The tape actually sounds like a good idea.  Thanks!


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