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OfflineDonate
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Registered: 07/23/15
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general questions
    #21986158 - 07/23/15 04:59 PM (8 years, 6 months ago)

new here, but have been researching mycology through the site for awhile.
i have a few questions that hopefully can be answered:

does pc'ing to sterilize jars become ineffective at a certain point in time, pressure or temperature? i ask because i am unable to gauge the psi on my pc therefore im unsure on the correct timing. can i just pc for 45 mins (max time allowed on pc) , then again for 25? or more? i tried 45 + 15 and had decent results, only contam was green mold.

distilled or spring water for BRF substrate? i used all distilled but had a minor accident where about 3 jars had spring instead. of these 3 at least 2 if not all 3 have rhizomorphic (spelling?) growth, whereas most others dont... an interesting observation for me.

and lastly, is there any reasons for not cutting the cake in half, either way now that i think about it (originally i thought lengthwise,) and putting it in the FC like that, verm down obviously? it just seems like there is more myc to examine on the bottom, and you can directly rewater the source.

thanks for keeping the site up guys, its a really great resource.


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OfflineMachiavelliavore
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Re: general questions [Re: Donate]
    #21986261 - 07/23/15 05:21 PM (8 years, 6 months ago)

Quote:

Donate said:
new here, but have been researching mycology through the site for awhile.
i have a few questions that hopefully can be answered:

does pc'ing to sterilize jars become ineffective at a certain point in time, pressure or temperature? i ask because i am unable to gauge the psi on my pc therefore im unsure on the correct timing. can i just pc for 45 mins (max time allowed on pc) , then again for 25? or more? i tried 45 + 15 and had decent results, only contam was green mold.

distilled or spring water for BRF substrate? i used all distilled but had a minor accident where about 3 jars had spring instead. of these 3 at least 2 if not all 3 have rhizomorphic (spelling?) growth, whereas most others dont... an interesting observation for me.

and lastly, is there any reasons for not cutting the cake in half, either way now that i think about it (originally i thought lengthwise,) and putting it in the FC like that, verm down obviously? it just seems like there is more myc to examine on the bottom, and you can directly rewater the source.

thanks for keeping the site up guys, its a really great resource.




PC times depend on what and how much you are cooking.  PF cakes I assume.  Are you using an electric pressure cooker?  Regardless, the point of long PC times is to get rid of bacteria, mold dies pretty easily.  The fact that you had it suggests it either took advantage of an area with bacterial contamination, your inoculation procedure or a shitty verm barrier introduced the contam, or your syringe was dirty.

Steaming PF cakes in a pot for 90 minutes is sufficient.  I imagine 65-70 @ whatever PSI will likely get the job done as well.  Do you have to open the cooker to add more water or just reset an electronic timer?

Just use tap water.

Re cutting them in half:  you'd just be disrupting the mycellial network for no reason.  I can't really think why you'd want to do it.  I suppose saucerwatering might work better.  Try it and show us that there is a reason to do it.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineDonate
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Re: general questions [Re: Machiavelliavore]
    #21986401 - 07/23/15 05:50 PM (8 years, 6 months ago)

thanks much for quick response

i cant trust los angeles tap water, ive heard you can contract hepatitis from even stepping in the water in the tunnels..

i had to resort to using the longer 8 oz jars, forgot to add that :crazy:

just seems because one side (bottom) isnt able to grow properly.


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OfflineMachiavelliavore
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Re: general questions [Re: Donate]
    #21986462 - 07/23/15 06:03 PM (8 years, 6 months ago)

You will be pressure boiling that water, that's about as clean as it's gonna get.  Are you talking about sewers or are you being sarcastic?  I lived in LA, drank tap erry day, still gotta liver to slay.  yeeeeeeeeee.... anyway

8oz is fine.  Just means you'll have a smaller cake.  The general colonization patterns is that you have  mycellial expolosion around the incoluation points, then creeping growth that unites the inital plooms.


How long has it been since spore germination?  You can always try slicing the shitty piece off the bottom.


What's the deal with your pressure cooker?  Electric?  You can always just steam your PF cakes if your PC is too much of a pain in the ass.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisiblebodhisattaMDiscordReddit
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Re: general questions [Re: Donate]
    #21986484 - 07/23/15 06:06 PM (8 years, 6 months ago)

LA tap water is fine for growing cubes. spring water is fine too, distilled water is only for lead acid batteries, humidifiers, and spore syringes.

if you get mold after sterilization, you're the culprit or your inoculant is the culprit. molds die at much lower temperatures than sterilization. even the most heat resistant fungal spores only survive 190F or so (byssochlamys spp.) any mold you would find in your jars would have died well before 190 even.

electric pressure cookers are for food not sterilization. buy a real pressure cooker. or steam your PF jars. PF/BRF cakes are acceptable to steam sterilize in a reasonable amount of time


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OfflineDonate
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Re: general questions [Re: Machiavelliavore]
    #21986512 - 07/23/15 06:12 PM (8 years, 6 months ago)

no, the water in question was for the brf substrate. obviously any water can be used for boiling. its an electric pc that has only preset times, not temperatures.
there is no shitty piece, just something i was wondering. it seems like all myc from the elongated 8oz jars, if cut in half longways, could all start growing somewhat upwards while more open to light/mist instead of perpendicular.


Edited by Donate (07/23/15 06:18 PM)


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OfflineDonate
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Re: general questions [Re: Donate]
    #21986528 - 07/23/15 06:14 PM (8 years, 6 months ago)

ok, noted.
since i am to blame for all of my contams, (not very many) the electric pc must work.
i would use a normal one but no access to stove.
thanks again


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InvisiblebodhisattaMDiscordReddit
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Re: general questions [Re: Donate]
    #21986547 - 07/23/15 06:20 PM (8 years, 6 months ago)

you can buy a hot plate for 20-40 dollars and that will run a PC.


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OfflineMachiavelliavore
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Re: general questions [Re: Donate]
    #21986548 - 07/23/15 06:20 PM (8 years, 6 months ago)

The water will reach boiling temperatures inside the PC and inside the sub...  Do you bake, cook pasta, or shower with bottled water?  I hope not.  What a waste of plastic.

As long as you don't need to open the electric PC, you should be fine.  Might want to do like 75 minutes to be safe, since 60 is what's usually recommended for cakes at 15psi.

You might consider (depending on how your jars are configured) giving a squirt of spore solution down the side of each jar to help spore solution reach the bottom if it's quite a tall substrate.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineDonate
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Re: general questions [Re: Machiavelliavore]
    #21986613 - 07/23/15 06:37 PM (8 years, 6 months ago)

wasn't aware the water in the sub would boil. sorry


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