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TravelAgency
The ongoing "wow"


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New to oysters - agar to WBS to bulk bucket
#21985015 - 07/23/15 02:05 PM (8 years, 6 months ago) |
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Hello all, though I am not new to mycology I am new to growing oysters. I am going to clone store bought oysters with agar, then transfer the wedge to sterilized WBS quarts in my SAB. Then I want to spawn to buckets as I am running out of room and I will be able to hang them from the wall.
My question is: would it be good to use a mostly coir substrate (pasteurized of course) with some pH balance and vermiculite, or would you recommend going all straw? From what I've read it seems most people prefer straw as their bulk sub. But I already have a lot of coir.
And any links to an actual Tek would be handy. I've only read what other people have done with only bits and pieces of information here and there so a full write up would be nice.
Edited to add: I'm also going to try to clone enokis- I know they are hard because of how difficult it is to get a clean culture with such skinny stems.
Edited by TravelAgency (07/23/15 02:07 PM)
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wildernessjunkie
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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#21985092 - 07/23/15 02:19 PM (8 years, 6 months ago) |
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I've had problems with straw in buckets. The center has gone anerobic on me multiple times.
I've never personally used coir for oysters but I've read that others have had poor results. Having never done it myself I won't say it works or not.
With my buckets, I have had excellent results with boiled wood chips. Colonization is fast. Contamination resistance is great. And I can usually pull off about 5 good flushes.
Gimme a min and Ill look for some links.
Edit: http://www.shroomery.org/forums/showflat.php/Number/20855770#20855770
http://www.shroomery.org/forums/showflat.php/Number/16699459
Edited by wildernessjunkie (07/23/15 02:25 PM)
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MudaFuka
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Registered: 12/14/13
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Yeah I wouldn't bother with an oyster grow on coir. Straw is the best choice for oyster IMO. I've had great results with a sterilised mix of sawdust and wheat bran but straw is just so easy to prep. I've used straw in buckets and much larger totes and gon past 5 flushes with no sign of contamination. http://www.shroomery.org/forums/showflat.php/Number/21690790
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TravelAgency
The ongoing "wow"


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Re: New to oysters - agar to WBS to bulk bucket [Re: MudaFuka]
#21985875 - 07/23/15 04:05 PM (8 years, 6 months ago) |
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Thanks you guys! Time to read read read!
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nooberst
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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#21985888 - 07/23/15 04:08 PM (8 years, 6 months ago) |
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I recently spawned oysters to mulch on a bucket
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Gr0wer
always improving


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Re: New to oysters - agar to WBS to bulk bucket [Re: nooberst]
#21986449 - 07/23/15 06:01 PM (8 years, 6 months ago) |
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A pointer on the buckets. Don't stack them especially for the first 1-2 weeks while they are colonizing. Once they are colonized the temps typically drop and they can be stacked. The core temp stacks and the bottom pail will be 20-30F higher than ambient. Stack them with wood between each pail if you do. I do pails but currently using mulch and manures.
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mushme



Registered: 10/10/10
Posts: 243
Loc: Washington
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Re: New to oysters - agar to WBS to bulk bucket [Re: Gr0wer]
#21987582 - 07/23/15 10:14 PM (8 years, 6 months ago) |
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I have been curious how coir would perform by itself so last week I made this bag using just coir verm and water then spawned with wbs.
 This was spawned on 7-17 with yellow oysters, i will let you know if it works.
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TravelAgency
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Re: New to oysters - agar to WBS to bulk bucket [Re: mushme]
#21988677 - 07/24/15 06:53 AM (8 years, 6 months ago) |
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Thanks! Interesting bag you got there- more pinning points?
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mushme



Registered: 10/10/10
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Loc: Washington
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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#21988783 - 07/24/15 07:49 AM (8 years, 6 months ago) |
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I use these bags mostly because I get them free form work.
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Gr0wer
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Re: New to oysters - agar to WBS to bulk bucket [Re: mushme]
#21991769 - 07/24/15 06:41 PM (8 years, 6 months ago) |
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I'm currently doing a wood chip with 20% coir no coir experiment. Although I'm having FAE and humidity issues with a recent swap from a cool mist to a pail with ultrasonic. So the experiment needs to be redone once I dial it back in.
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TravelAgency
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Re: New to oysters - agar to WBS to bulk bucket [Re: Gr0wer]
#21991777 - 07/24/15 06:43 PM (8 years, 6 months ago) |
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Are oysters like other subs where you mostly provide moisture through sub field capacity? Or do I need to introduce humid conditions/mist?
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TravelAgency
The ongoing "wow"


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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#22004577 - 07/27/15 01:43 PM (8 years, 6 months ago) |
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My samples are starting to recover on my agar plates! Oysters here I come! Will post pics when they've grown enough to do isolates.
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Tunnel_Bunny
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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#22013219 - 07/29/15 03:40 AM (8 years, 6 months ago) |
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I am buying my supplies currently to grow oysters in a bucket. Good luck to you as well!
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TravelAgency
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Re: New to oysters - agar to WBS to bulk bucket [Re: Tunnel_Bunny]
#22014189 - 07/29/15 10:16 AM (8 years, 6 months ago) |
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Thanks! You as well!
My current PC can only fit one qt jar and still operate so until I can afford a new one (had only done PF Tek in the past, it could fit 14 1/2 pints) it will be slow work but I'm super excited.
Are you starting with agar as well?
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MudaFuka
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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#22014215 - 07/29/15 10:22 AM (8 years, 6 months ago) |
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Why not do grain in 1/2 pints. I used 24 1/2 pints of WBS for my first grow.
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TravelAgency
The ongoing "wow"


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Re: New to oysters - agar to WBS to bulk bucket [Re: MudaFuka]
#22014257 - 07/29/15 10:30 AM (8 years, 6 months ago) |
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Honestly that seems like way more of a hassle than just waiting to buy a bigger PC. I only have so many agar plates- was planning on g2g- wouldn't that make g2g difficult? And also reduce how far you can take an inoculation?
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MudaFuka
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Registered: 12/14/13
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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#22014275 - 07/29/15 10:33 AM (8 years, 6 months ago) |
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Making a grain jar is no more work than making a PF cake. G2G is easier with smaller jars. Just a suggestion though. Do what you want. When I used 1/2 pints I used 1 1/2 pint to inoculate 12 via G2G.
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Tunnel_Bunny
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Re: New to oysters - agar to WBS to bulk bucket [Re: MudaFuka]
#22014295 - 07/29/15 10:36 AM (8 years, 6 months ago) |
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I have no source to clone so I have to buy grain spawn. I got the 23 quart presto pc brand new. It's huge. I'm going to put 5 gallon buckets worth of hay in it once I have the spawn ready. Gunna pc some wbs probably like 5-6 quarts per 5 gallon bucket. Thinking about making 6 buckets for marketing locally
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TravelAgency
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Re: New to oysters - agar to WBS to bulk bucket [Re: MudaFuka]
#22014342 - 07/29/15 10:45 AM (8 years, 6 months ago) |
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Well I might give it a go... About to birth 14 cakes so I would have the spare room. What kind of filter did you use for them? I've got polyfil and micropore tape- need to wait to get paid to get sfd.
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TravelAgency
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Re: New to oysters - agar to WBS to bulk bucket [Re: Tunnel_Bunny]
#22014348 - 07/29/15 10:46 AM (8 years, 6 months ago) |
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I just cloned store bought oysters. If you are near any Asian markets the generally have a plethora of mushrooms to choose from
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Tunnel_Bunny
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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#22014400 - 07/29/15 11:02 AM (8 years, 6 months ago) |
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just poke a hole in the lid push polyfill thru and mix in grain spawn right?
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TravelAgency
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Re: New to oysters - agar to WBS to bulk bucket [Re: Tunnel_Bunny]
#22014480 - 07/29/15 11:24 AM (8 years, 6 months ago) |
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That's what I have for my qt jars, though I do want to upgrade to sfd- but my 1/2 pints already have the 4 inoculation sized holes (dry verm over brf). I was more curious what Muda did personally.
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MudaFuka
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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#22017219 - 07/29/15 08:55 PM (8 years, 5 months ago) |
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I used a 1/4" hole stuffed tightly with polly for about a year. I switched to a double layer of EZ felt after I started making 72 qts a week. I stuffed poly into 100 lids in one sitting and said fuck poly after that. Now I use a small piece of SFD siliconed over a 1/4" hole. Poly-fill works great it's just a pain to stuff.
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nooberst
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Re: New to oysters - agar to WBS to bulk bucket [Re: MudaFuka]
#22017379 - 07/29/15 09:26 PM (8 years, 5 months ago) |
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I hate stuffing polyfil I'm going to order some sfd
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Gr0wer
always improving


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Re: New to oysters - agar to WBS to bulk bucket [Re: nooberst]
#22017911 - 07/29/15 11:46 PM (8 years, 5 months ago) |
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I made the swap to EZ felt as well. I got the heavy duty felt that's twice as thick. I don't use glue either, i melt a hole using a torched hot socket and use the melted ring of plastic to hold the felt in place.
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TravelAgency
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Re: New to oysters - agar to WBS to bulk bucket [Re: Gr0wer]
#22019433 - 07/30/15 11:44 AM (8 years, 5 months ago) |
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Cool thanks
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Tunnel_Bunny
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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#22024435 - 07/31/15 01:43 PM (8 years, 5 months ago) |
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ok so i got my 6lb of blue oyster spawn ordered. it should be here in a week or two. i am PCing seven quarts half filled with WBS so hopefully i can turn 6lb of spawn (7quarts) into 14 quarts. i might run out and pick up another case of jars.... how many quart jars; ratio 1:1 of blue oyster grain spawn will it take to inoc 6 buckets of straw??
i am going to use the plastic bag in the bucket teqnique to start the oysters off well with little holes poked through for gas exchange.
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Gr0wer
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Re: New to oysters - agar to WBS to bulk bucket [Re: Tunnel_Bunny]
#22024462 - 07/31/15 01:54 PM (8 years, 5 months ago) |
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I use two quart jars per five gallon pails with good results. It's fully colonized in 8-12 days.
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Tunnel_Bunny
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Re: New to oysters - agar to WBS to bulk bucket [Re: Gr0wer]
#22024677 - 07/31/15 02:48 PM (8 years, 5 months ago) |
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cool so i should be able to turn 6lb of spawn into 12 quarts of spawn for making 6 buckets of straw for oysters.
nice
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bryanbzl
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Re: New to oysters - agar to WBS to bulk bucket [Re: Tunnel_Bunny]
#22049523 - 08/05/15 07:05 PM (8 years, 5 months ago) |
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Hey Travel,
Thought I'd give you something easy try. Check out this link. The substrate is "scotts" brand mulch. you can find it pretty much anywhere in north America. It is cheap and comes already hydrated. by far the easiest and cheapest way to go for a beginner. I have also found that wetting it further only leads to possible trich. If the bag comes punctured and seems dry (never had this happen), then I would soak it and then let it sit till the surface is bone dry. Check it out. you wont regret it.
http://www.shroomery.org/forums/showflat.php/Number/21744280
any questions shoot me a PM directly.
-------------------- Cheers, bzl -------------------------------------------------------------------------------------------------------------------------------------------------------------------------- "From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children." conclusion: poor fucking children of the early 1900's.
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bryanbzl
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Re: New to oysters - agar to WBS to bulk bucket [Re: bryanbzl]
#22049576 - 08/05/15 07:17 PM (8 years, 5 months ago) |
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Quote:
bryanbzl said: Hey Travel,
Thought I'd give you something easy try. Check out this link. The substrate is "scotts" brand mulch. you can find it pretty much anywhere in north America. It is cheap and comes already hydrated. by far the easiest and cheapest way to go for a beginner. I have also found that wetting it further only leads to possible trich. If the bag comes punctured and seems dry (never had this happen), then I would soak it and then let it sit till the surface is bone dry. Check it out. you wont regret it.
http://www.shroomery.org/forums/showflat.php/Number/21744280
any questions shoot me a PM directly.
Forgot to mention.
I have done this in buckets/tubs/bags/etc. I don't think there is a situation where you cannot use this substrate.
Also, I have switched from WBS to Hard red winter wheat. I have never tried Rye because it isn't really common here. But from what I can tell it is similar. Make sure it is HARD RED WINTER WHEAT and not the SOFT type wheats as they tend to be gooey. I have had way better results since switching. I attribute this to an increase in airflow around the kernels in the jar. WBS kernels are so small that they matt together in the jar and make a much more favourable environment for the organisms you want to avoid. The trade off is less inoculation points. but to be honest I think anything smaller then wheat/rye doesn't seem to make a noticeable difference to warrant using it. Also the spawn breaks up a whole lot easier and when you need to break up a bunch of jars in one sitting... smacking them on a tire for an hour isn't fun when you can whack rye or wheat spawn two times and have perfectly broken up spawn ready to go. Unless your dead broke and really can't spend the extra cents on rye or wheat. I'd highly recommend avoiding WBS. I have used it for almost a decade and although it works... I wish I switch a long time ago.
-------------------- Cheers, bzl -------------------------------------------------------------------------------------------------------------------------------------------------------------------------- "From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children." conclusion: poor fucking children of the early 1900's.
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
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Re: New to oysters - agar to WBS to bulk bucket [Re: bryanbzl]
#22049814 - 08/05/15 07:52 PM (8 years, 5 months ago) |
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Quote:
bryanbzl said:
Quote:
bryanbzl said: Hey Travel,
Thought I'd give you something easy try. Check out this link. The substrate is "scotts" brand mulch. you can find it pretty much anywhere in north America. It is cheap and comes already hydrated. by far the easiest and cheapest way to go for a beginner. I have also found that wetting it further only leads to possible trich. If the bag comes punctured and seems dry (never had this happen), then I would soak it and then let it sit till the surface is bone dry. Check it out. you wont regret it.
http://www.shroomery.org/forums/showflat.php/Number/21744280
any questions shoot me a PM directly.
Forgot to mention.
I have done this in buckets/tubs/bags/etc. I don't think there is a situation where you cannot use this substrate.
Also, I have switched from WBS to Hard red winter wheat. I have never tried Rye because it isn't really common here. But from what I can tell it is similar. Make sure it is HARD RED WINTER WHEAT and not the SOFT type wheats as they tend to be gooey. I have had way better results since switching. I attribute this to an increase in airflow around the kernels in the jar. WBS kernels are so small that they matt together in the jar and make a much more favourable environment for the organisms you want to avoid. The trade off is less inoculation points. but to be honest I think anything smaller then wheat/rye doesn't seem to make a noticeable difference to warrant using it. Also the spawn breaks up a whole lot easier and when you need to break up a bunch of jars in one sitting... smacking them on a tire for an hour isn't fun when you can whack rye or wheat spawn two times and have perfectly broken up spawn ready to go. Unless your dead broke and really can't spend the extra cents on rye or wheat. I'd highly recommend avoiding WBS. I have used it for almost a decade and although it works... I wish I switch a long time ago.
Ok, sweet! Thanks for the info! Just did some agar to agar transfers today! I'm stoked!
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mushme



Registered: 10/10/10
Posts: 243
Loc: Washington
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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#22175421 - 09/01/15 07:40 PM (8 years, 4 months ago) |
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Update on the coir verm. log, I would not waste your time with the coir here is my first flush.

I did start with spores so it could be partly genetics but it seems they are getting very little from the coir.
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TravelAgency
The ongoing "wow"


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Re: New to oysters - agar to WBS to bulk bucket [Re: mushme]
#22179247 - 09/02/15 05:45 PM (8 years, 4 months ago) |
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Thanks for the update! Yeah I found a place with really cheap wheat straw so I'm going that route. One of my master jars is almost 100%! It'll be soon.
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mushme



Registered: 10/10/10
Posts: 243
Loc: Washington
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Re: New to oysters - agar to WBS to bulk bucket [Re: TravelAgency]
#22179739 - 09/02/15 07:19 PM (8 years, 4 months ago) |
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No problem, good luck with your grow I look forward to seeing your results.
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midnightmaraude
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Re: New to oysters - agar to WBS to bulk bucket [Re: mushme]
#22182306 - 09/03/15 10:00 AM (8 years, 4 months ago) |
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Quote:
mushme said: No problem, good luck with your grow I look forward to seeing your results.
Is your bag in a greenhouse type set up or just laid out somewhere?
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mushme



Registered: 10/10/10
Posts: 243
Loc: Washington
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Re: New to oysters - agar to WBS to bulk bucket [Re: midnightmaraude]
#22183048 - 09/03/15 01:12 PM (8 years, 4 months ago) |
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It's just laying on a counter.
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eatyualive
Eat's You Alive :)



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Re: New to oysters - agar to WBS to bulk bucket [Re: mushme]
#22202889 - 09/07/15 04:12 PM (8 years, 4 months ago) |
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