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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Offlineuninc4life2010
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Is it better to inoculate WBS jars with LC made from agar, or inoculate with an agar wedge?
    #21984727 - 07/23/15 01:12 PM (8 years, 6 months ago)

I was just asking which method you prefer?  It seems like the LC could stretch your agar further, but would be more susceptible to contamination.  The LC would still need to be tested for sterility with a jar before it could be used.  Which do you all recommend?  I have only used LC from multispore with iffy results in the past, and I am still a bit cautious when it comes to LC's.  But, I have been told that it is much better to inoculate an LC with agar, as the wedge can be inspected for contams before use. Any suggestions would be great.  Thanks!


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InvisibleJuiceh
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Re: Is it better to inoculate WBS jars with LC made from agar, or inoculate with an agar wedge? [Re: uninc4life2010]
    #21984780 - 07/23/15 01:16 PM (8 years, 6 months ago)

LC from agar is the most reliable way to make a clean LC. But agar wedges are the most reliable way to inoculate grains. If you inoculated your LC in aseptic conditions and your sterile technique was good and your hands didn't float around over the open LC jar, the LC should have a very high chance of being clean. You should still test it on a petri dish though.


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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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