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Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Liquid Cultures

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Offlinebakenast
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Registered: 03/14/11
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GLC question is this ok to do
    #21984234 - 07/23/15 11:44 AM (8 years, 6 months ago)

im needing to make some lc syringes to inoculate some more jars, I made small 1/2 pint grain jar with injector port just to make some lc syringes,  my question is, is it ok to go ahead and make add my sterile water and make my syringes if 95% of the jar is colonized?  its colonized quickly but I wont be availaible to wait for it to get to 100%, is this ok to do even though about 5% is uncolonized?  thanks


Edited by bakenast (07/23/15 12:04 PM)


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OfflinePsilosoulful

Registered: 09/05/14
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Re: LC question is this ok to do [Re: bakenast]
    #21984238 - 07/23/15 11:46 AM (8 years, 6 months ago)

It would be taking a risk, but when will you be back to check on the jars again? I would let it colonize to 100%, then when you come back break up the grains and inject the sterile water. It's a GLC btw.


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Offlinehamloaf
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Registered: 12/23/09
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Re: LC question is this ok to do [Re: bakenast]
    #21984265 - 07/23/15 11:49 AM (8 years, 6 months ago)

You are talking about extracting grain mycelium water from mycelium on grains AKA "GLC/Grain Liquid Culture" which isn't a liquid culture, moreover, an extraction method using water as the media transfer catalyst and syringes as the media transfer vessel. 

Instead of that, sterilize receiving jars full of grain and transfer colonized grains from your master jar into your freshly sterilized jas inside of a still air box.


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Offlinebakenast
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Re: LC question is this ok to do [Re: Psilosoulful]
    #21984288 - 07/23/15 11:54 AM (8 years, 6 months ago)

its the fact that I have to work tmmrw and i travel, if I could just make my syringes today i could inoculate more jars or wait for ten days, theres not enough grains to do a GTG its only a 1/2 pint about 1/2 full,  I know what GLC is i just miss posted


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