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InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Very odd occurance in BRF jar.
    #21982928 - 07/23/15 01:39 AM (8 years, 6 months ago)

So this BRF jar just reached full colonization 2-3 days ago. Something has started to form in the dry verm layer at the top. I checked the jar 24 hours ago and there was nothing. I checked 30 minutes ago and found this:





Is this mushroom formation or what? I have no idea what this is, but upon close inspection it has the appearance of a mushroom's stem. I know my pictures aren't perfect, but they are the best my camera can provide. I'm hoping you guys can inform me what this is, and also what I should do with it. If it is a mushroom should I dunk and roll the cake and place it in SGFC ASAP or what?


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InvisibleToadstool5
A Registered Mycophile
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Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
Re: Very odd occurance in BRF jar. [Re: Dr.Satan]
    #21982937 - 07/23/15 01:43 AM (8 years, 6 months ago)

Its primordia forming into pins! :cheers:

I would personally dunk, roll, and pace in fruiting conditions :thumbup:

How deep is your verm layer? It looks odd


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If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

AMU Teks :mushroom2: Stro's Write Ups


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InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: Very odd occurance in BRF jar. [Re: Toadstool5]
    #21982942 - 07/23/15 01:45 AM (8 years, 6 months ago)

This jar in particular didn't have enough sub so I had to make the layer like 1 inch rather then 1/2 inch. And the coloring is a bit off due to the lighting. Anyways I will begin the dunk and roll now <3, many thanks buddy.

Edit - the 1 inch number was more of an estimate. The dry verm layer was probably somewhere between 1-2 inches. Either way it seems to have worked out all the same.


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Edited by Dr.Satan (07/23/15 02:17 AM)


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InvisibleToadstool5
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Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
Re: Very odd occurance in BRF jar. [Re: Dr.Satan]
    #21982959 - 07/23/15 02:00 AM (8 years, 6 months ago)

:goodluck:

It shouldnt be an issue, I was worried it was uncolonized substrate.


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

AMU Teks :mushroom2: Stro's Write Ups


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InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: Very odd occurance in BRF jar. [Re: Dr.Satan]
    #21982971 - 07/23/15 02:09 AM (8 years, 6 months ago)

Ah, when I took it out of the jar it smelled like fresh mushies. It's amazing! I can't help but feel proud :grin:


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Very odd occurance in BRF jar. [Re: Dr.Satan]
    #21983216 - 07/23/15 05:54 AM (8 years, 6 months ago)

:vibin: I would definitely clone one of those pins and get a print or two from the ones you let mature. Great job!


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InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: Very odd occurance in BRF jar. [Re: insanemike]
    #21983298 - 07/23/15 06:32 AM (8 years, 6 months ago)

Quote:

insanemike said:
:vibin: I would definitely clone one of those pins and get a print or two from the ones you let mature. Great job!




I plan to get as many prints as possible from this first flush :laugh:. And you mean clone those little pins now? I didn't know that was possible I thought you could only clone full grown mushies, but if you think I should I'll give it a shot.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Very odd occurance in BRF jar. [Re: Dr.Satan]
    #21983433 - 07/23/15 07:28 AM (8 years, 6 months ago)

Damn right. Don't have to clone them all. Just take one pin and put it on a few plates. The fact that it's pinning invitro must say something about its genetics.


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InvisibleToadstool5
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Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
Re: Very odd occurance in BRF jar. [Re: insanemike]
    #21983964 - 07/23/15 10:23 AM (8 years, 6 months ago)

Yeah dude primordia have all the cells they will have until maturation. The cells of the fruit body only elongate rather than divide.

I've seen guys place an entire pin on an agar plate before. I would personally only do that after a soak in dilute bleach though because of exposure to open air.

In vitro pins can be a sign of full consolidation too, since this cake was smaller it consolidated quickly and now feels spatially and nutritionally stressed so its trying to reproduce and spread to new substrate.


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

AMU Teks :mushroom2: Stro's Write Ups


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Very odd occurance in BRF jar. [Re: Toadstool5]
    #21983984 - 07/23/15 10:27 AM (8 years, 6 months ago)

I agree with everything you said but when you have invitro pins, atleast for me, seems like if they are willing to fruit in less then favorable conditions then they may and have IME performed rather well when cloned.


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InvisibleToadstool5
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Male User Gallery


Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
Re: Very odd occurance in BRF jar. [Re: insanemike]
    #21984099 - 07/23/15 11:03 AM (8 years, 6 months ago)

A great point! The mycellial strand that knotted up first is probably morphologically stronger than the rest and is more CO2 tolerant as well.

I need to start cloning primordia more often :awesomenod:


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

AMU Teks :mushroom2: Stro's Write Ups


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InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: Very odd occurance in BRF jar. [Re: Toadstool5]
    #21993726 - 07/25/15 05:05 AM (8 years, 6 months ago)

This cake has 3 pins already from over night :smile:. I am wondering if turning a fan on by my SGFC for 30min a day would be an easier way to replace fanning. Does anyone know if this is a good idea? I could do it for less time, but my goal is simply to avoid having to fan by hand lol.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Very odd occurance in BRF jar. [Re: Dr.Satan]
    #21993792 - 07/25/15 05:55 AM (8 years, 6 months ago)

Do not use a fan and especially not for 30 minutes. You will dry your cakes out. When we manually fan, it's only for 30 seconds. If you don't want to do the work that is involved then maybe this hobby isn't for you. If and when you move up to bulk, the tubs themselves might for the most part be set and forget but the agar work, grain prep, tub building, cleaning, planning and everything else I can't think of right now, will keep you busy 7 days a week. I don't do pf cakes because there not worth my time and would end up being neglected but if pf cakes were what I was cultivating exclusively, I would be on those sum bitches like a hawk.


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InvisibleDr.Satan
Mad Professor
I'm a teapot


Registered: 06/26/15
Posts: 2,182
Re: Very odd occurance in BRF jar. [Re: insanemike]
    #21993830 - 07/25/15 06:26 AM (8 years, 6 months ago)

Quote:

insanemike said:
Do not use a fan and especially not for 30 minutes. You will dry your cakes out. When we manually fan, it's only for 30 seconds. If you don't want to do the work that is involved then maybe this hobby isn't for you. If and when you move up to bulk, the tubs themselves might for the most part be set and forget but the agar work, grain prep, tub building, cleaning, planning and everything else I can't think of right now, will keep you busy 7 days a week. I don't do pf cakes because there not worth my time and would end up being neglected but if pf cakes were what I was cultivating exclusively, I would be on those sum bitches like a hawk.




Ah, see 30 seconds isn't bad at all! I read a post by someone on these forums that said "if anyone is only fanning for 30 seconds that's not going to do anything" or something along those lines. So I was given the impression I was meant to fan them like 10 minutes by hand or something lol. If it's only 30 seconds then that's not bad at all. I can manage 30 second fannings 3 times a day without any issue. Is 3 a good number?


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Very odd occurance in BRF jar. [Re: Dr.Satan]
    #21993867 - 07/25/15 06:48 AM (8 years, 6 months ago)

Only fan after misting and only mist when needed (not on a time schedule). We fan to promote (kickstart) evaporation of the water we just misted onto the cake. A good 30 seconds to a minute will suffice.


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
Re: Very odd occurance in BRF jar. [Re: insanemike]
    #21994029 - 07/25/15 08:13 AM (8 years, 6 months ago)

Quote:

insanemike said:
I agree with everything you said but when you have invitro pins, atleast for me, seems like if they are willing to fruit in less then favorable conditions then they may and have IME performed rather well when cloned.




I don't have much experience with brf cakes but, I was under the impression that most all cakes will pin invitro eventually.  I'm guessing this is because the amount of GE is higher than in a normal quart spawn jar.  I did about a dozen recently with MS and they all pinned invitro, some took longer than others.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Very odd occurance in BRF jar. [Re: invitro]
    #21994112 - 07/25/15 08:44 AM (8 years, 6 months ago)

Brf cakes are far more nutritious than agar and usually take 2-4 weeks to colonize and yes good genetics will prove to not last the duration of a consolidation perdiod before pinning. Remember the #1 pinning trigger is full colonization. Light and evaporation are secondary pinning triggers.

If you have an ms plate from spores grow invitro mushrooms 2 -3 weeks after inoculation, one would have to think they are the best genetics on that plate.  So at that point I would transfer that invitro mushroom to a few more plates and than use the rest of the plate to get some grain going. Wouldn't you agree?


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Offlinekiksen
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Registered: 06/04/15
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Last seen: 2 months, 25 days
Re: Very odd occurance in BRF jar. [Re: insanemike]
    #21994710 - 07/25/15 11:03 AM (8 years, 6 months ago)

That looks really good!


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