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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 21 hours, 2 minutes
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new to agar petri dishes, two questions.
#21980921 - 07/22/15 04:33 PM (8 years, 6 months ago) |
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Hello,
I've been using agar petri for a few months with a still air box. I think I have my kinks worked out, things are growing cleanly.
My goal is to fill a few large 60cc syringes with LI to noc up myco bags.
questions:
#1 I have one petri dish untaped inside each 5"x8" ziplock bag. I intend to grow it to cover the surface before I make the LI syringe. Will that be enough air? Or should I open it once in the still air box to refresh the air?
#2 My plan is to pull up the whole agar disk, and put it in a sterile jar with glass pieces and a self-healing lid, inject sterile syringe water into it and shake and suck up the LI. Good plan? (I worry about all that agar mixing in with the LI)
Thanks!!
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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UncleFester
Lord Moldy-Butt



Registered: 09/23/14
Posts: 460
Loc: Where The Sun Don't Shine...
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Re: new to agar petri dishes, two questions. [Re: tedoro]
#21981462 - 07/22/15 06:37 PM (8 years, 6 months ago) |
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Quote:
tedoro said: #2 My plan is to pull up the whole agar disk, and put it in a sterile jar with glass pieces and a self-healing lid, inject sterile syringe water into it and shake and suck up the LI. Good plan? (I worry about all that agar mixing in with the LI)
Sounds interesting honestly, I'd love to see some pictures of the process.
I don't think you'll need to open up the SAB if you use the disinfectant spray moderately
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Looking for Penis Envy & Psilocybe Cubensis Cuba spores.
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Alphacheese
Stranger
Registered: 07/22/15
Posts: 3
Last seen: 8 years, 5 months
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Re: new to agar petri dishes, two questions. [Re: tedoro]
#21981486 - 07/22/15 06:41 PM (8 years, 6 months ago) |
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#1 If your goal is to grow out the dish and "harvest" when full you don't need to provide any air exchange. The best setup of a petri dish is to wrap parafilm around the edge anyway, sealing it up to prevent any contamination from getting in. I imagine that's why they're in zip top bags? If you have a clean place to incubate I wouldn't even put them in zip bags unless you know they are very "clean".
#2 Your plan for making LI sounds interesting, very armature. I don't mean that to be insulting, I mean ingenuitive, gotta work with what you got. Shaking it around with glass shards might cause to much damage to your mycelium and you'll be left with less viable inoculum. That's only a guess on my part, I'd like to hear how those results work out. I'm afraid you won't get enough good mixing of the agar and mycelium into your LI in order to draw it up into the syringe. I've thought of modifying a magic bullet blender and cup to make LI. You want as much mixing as possible to happen in the shortest amount of time as possible to prevent damaging your inoculum.
Edited by Alphacheese (07/22/15 06:43 PM)
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 15 hours
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Re: new to agar petri dishes, two questions. [Re: Alphacheese]
#21981968 - 07/22/15 08:46 PM (8 years, 6 months ago) |
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A modified blender is the method of choice for LI these days, check out Muda's LI thread.
Lately people have been making makeshift LI in ways similar to what you describe, you be best to mix up some no pour agar in half/quarter pint masons with agar in the bottom, inoculate with a transfer from a plate and when its grown out inject the sterile water.
But why LI? The way I work is agar wedge - master quart jar - G2G into 4qt spawn.
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tedoro
ToadStool Tender



Registered: 02/06/15
Posts: 2,206
Last seen: 21 hours, 2 minutes
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Re: new to agar petri dishes, two questions. [Re: mushpunx]
#21983970 - 07/23/15 10:24 AM (8 years, 6 months ago) |
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Thanks all ~
Good to know that there is enough air in the petri alone to grow it out fully. I thought I read that it can stall, but I was wrong.
My initial plan for doing this was to grow it out on the dish and put the mycelia in the jar.... but after working with agar for a while, I realize that I can't separate the growth from the agar. So thats why I am recalibrating my plan.
Some people squirt syringe water directly on the petri dish and make LI that way. That sounds tedious.
I'm attempting to avoid messing around with blenders... I'm already dorking around too much with this recreation.
The reason I am trying to make a simple LI is that I absolutely love pre-sealing myco bags. I can get 4 monotubs worth of grain in one PC load. Its amazing. So my clean mycelia needs to find its way into my pre-sealed mycobag.
The no-pour agar in a container I can directly mount to a blender.... that does sound just about perfect for my project. Maybe I need to take the plunge and learn that.
T
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: new to agar petri dishes, two questions. [Re: tedoro]
#21983975 - 07/23/15 10:26 AM (8 years, 6 months ago) |
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Quote:
The way I work is agar wedge - master quart jar - G2G into 4qt spawn.
QFT
old school ain't bad school kids!
slow and steady spawn production FTW
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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