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Offlineuninc4life2010
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Is it feasible to inoculate spawn bags in a glovebox?
    #21977436 - 07/21/15 08:29 PM (8 years, 6 months ago)

There were a couple methods I was going to try, but I have been told that spawn bags work much better in front of a flow hood.  Since I don't have a flow hood, the two methods I was considering would be to 1) either inoculate through an injection port in the bag with a spore or LC syringe, or 2) cut the top of the bag open right below the seal, insert an agar wedge, then zip tie the bag closed inside the glovebox. 

Do you all have any suggestions?


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InvisibleSteveRogers
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Re: Is it feasible to inoculate spawn bags in a glovebox? [Re: uninc4life2010]
    #21977460 - 07/21/15 08:34 PM (8 years, 6 months ago)

You could make an HLC and just inject normally inside a SAB
RTV silicone makes a nice injection port.

http://www.shroomery.org/forums/showflat.php/Number/21627998#21627998


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Edited by SteveRogers (07/22/15 10:23 PM)


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Invisiblemushmagic
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Re: Is it feasible to inoculate spawn bags in a glovebox? [Re: SteveRogers]
    #21978121 - 07/21/15 11:09 PM (8 years, 6 months ago)

You can inoculate bags in a SAB just fine. You dont need flowhood.


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