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OfflineMr.Top_Hat
The guy with a hat


Registered: 05/07/12
Posts: 114
Last seen: 3 years, 4 months
Issues with agar germination
    #21976401 - 07/21/15 04:41 PM (8 years, 6 months ago)

Hey everyone, so about 3 weeks ago I started my adventure in agar. I am using pastys tek and I have made up over the course of the first week about 40 plates. I am having a very hard time getting anything to germinate not even a contam as I can tell. I have used different ways of inoculation and agar mixes. My first two batches of agar where done like this.

Agar: 2.5 grams
Potato flakes: 3.3 grams
Light karo: 1.6 grams
Tap water: 1/2 cup
inoculation: syringe


My third batch was this.

Agar: 2 grams
Potato flakes: 3.3 grams
Light karo: 1.6 grams
Tap water: 1/2 cup
inoculation: print

Now my last batch was done without measuring but using pasty's guide on how he does it I also use a dif print for this one. Each batch I did the condensation inside of the dish was less and less. I feel I got the mixing of the agar correct maybe I didnt I am just trying to narrow down what my issue might be. I am confidant in my sterile tek as I have been growing for a few years now. I could be wrong with that as I am a complete noob to agar. The only thing to change is I recentlly moved but before I even set up my work station and grow area thoughly cleaned the room even ripped out the brand new carpet that was in there and spent a lot of money having hard wood flooring installed steam cleaned the room and sprayed for mold gave it a month. All this was done a month before I even moved into my house I then cleaned my work room again. I am just stumped the last thing I should mention is my spores are a year old taken from my last grow and the syringe was bought from a vendor I have no idea how old it is but all where stored for a year in a tote in a basement. Thanks for the help guys. Here is a pic of what I got going at the moment.

Pics will have to come later I can't post them for some reason on my tablet.


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