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OfflineShoegaze
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Registered: 03/30/15
Posts: 5
Last seen: 8 years, 4 months
Do I really need to make a mushroom print?
    #21974030 - 07/21/15 02:02 AM (8 years, 6 months ago)

Hi. I have successfully been growing shrooms from the PF Tek method for a while now. I want to start making my own spore syringes to save me a bit of money.

My questions is, do I really need to make a spore print first and then transfer the spores to the syringe? It seems like an unnecessary step to me.

My Idea is just to place a mushroom over a small sterile container full of sterile water, inside a sterile glove box, and let the spores just fall in. Then I quickly remove the shroom, cover the container with a foil lid, shake it, then suck the spore solution out with the syringe to inoculate my next batch.

I am i being quite naive that this might work.


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Offlinesheptownboi
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Registered: 07/13/15
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Re: Do I really need to make a mushroom print? [Re: Shoegaze]
    #21974046 - 07/21/15 02:11 AM (8 years, 6 months ago)

It might just be case of 'if it aint broke don't fix it'. the spore print works so id just stick to that. But i don't know shit so do whatever. :thumbup:


--------------------
'If you correct your mind, the rest of your life will fall into place' -Lao Tzu


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OfflineMachiavelliavore
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Re: Do I really need to make a mushroom print? [Re: Shoegaze]
    #21974076 - 07/21/15 02:33 AM (8 years, 6 months ago)

1/4 to 1/8th of a spore print would typically be used in a syringe.  That print was made over 12 to 24 hours.  It'd take 1.5 to 6 hours of holding the mushroom over water to get that spore volume.

It would also be harder to tell if/how much the mushroom was sporulating.  You'd need a piece of screen or skewer to hold the mushroom.  If pieces of substrate/mush/dirt fell off it, you couldn't go around it or remove it.

The best print you can take is on steamed/pc'd foil, taken from an invitro grow only opened in an airbox

http://www.shroomery.org/forums/showflat.php/Number/19035259/fpart/1/vc/1
see sterile spore printing

Otherwise the ziplock bag tek is probably the cleanest and easiest if you don't have an airbox.

Standard steamed foil prints, done in a sanitized container opened in still air conditions as breifly as possible should do fine, though I suggest you make the syringe in an airbox.


Using muda's LI tek with the blender half-pint from a good clone would be way better than a sterile print and only slightly more work.
http://www.shroomery.org/forums/showflat.php/Number/20429745/page/1 ; Liquid Inoculant
http://www.shroomery.org/forums/showflat.php/Number/19208976 ;         Agar


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: Do I really need to make a mushroom print? [Re: sheptownboi]
    #21974077 - 07/21/15 02:34 AM (8 years, 6 months ago)

Just make a syringe like everyone else.

1/5 of a print, or even 1/10 of a print is enough for a syringe.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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OfflineOPB
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Registered: 05/30/15
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Re: Do I really need to make a mushroom print? [Re: Machiavelliavore]
    #21974079 - 07/21/15 02:36 AM (8 years, 6 months ago)

Just drop the mushroom cap in a shallow empty jar...close it...wait 24 hours...remove cap...add water...shake...suck into syringe.


--------------------
"Wasn't that fun"


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InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: Do I really need to make a mushroom print? [Re: Machiavelliavore]
    #21974082 - 07/21/15 02:36 AM (8 years, 6 months ago)

Quote:

Machiavelliavore said:
The best print you can take is on steamed/pc'd foil, taken from an invitro grow only opened in an airbox




O you could just use foil straight off the roll and achieve the same results:shrug:

Foil is sterile off the roll. Don't make things more complicated than they need to be.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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OfflineDensePlacebo
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Registered: 05/14/15
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Re: Do I really need to make a mushroom print? [Re: OPB]
    #21974087 - 07/21/15 02:46 AM (8 years, 6 months ago)

Quote:

OPB said:
Just drop the mushroom cap in a shallow empty jar...close it...wait 24 hours...remove cap...add water...shake...suck into syringe.



This is what I do. I have one jar with a SHIP and that's it. I take a syringe flame the tip suck up water shoot into jar, repeat with how ever many syringes you wanna make( I do five per print), and your done.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!




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OfflineMatt87
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Registered: 01/03/15
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Re: Do I really need to make a mushroom print? [Re: DensePlacebo]
    #21974101 - 07/21/15 03:12 AM (8 years, 6 months ago)

I'd just quit the syringe nonsense altogether and get into some g2g transfers. You have some experience now. Go for it.


--------------------

Once you understand the way broadly, you see it in all things. -Musashi


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InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: Do I really need to make a mushroom print? [Re: Matt87]
    #21974147 - 07/21/15 03:55 AM (8 years, 6 months ago)

Quote:

Matt87 said:
I'd just quit the syringe nonsense altogether and get into some g2g transfers. You have some experience now. Go for it.



He never said he was moving onto grain.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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OfflineShoegaze
Stranger
Registered: 03/30/15
Posts: 5
Last seen: 8 years, 4 months
Re: Do I really need to make a mushroom print? [Re: OPB]
    #21974172 - 07/21/15 04:20 AM (8 years, 6 months ago)

Quote:

Just drop the mushroom cap in a shallow empty jar...close it...wait 24 hours...remove cap...add water...shake...suck into syringe.

This is what I do. I have one jar with a SHIP and that's it. I take a syringe flame the tip suck up water shoot into jar, repeat with how ever many syringes you wanna make( I do five per print), and your done.




Isn't there a risk of getting contamination from the mushroom cap? Should I use a glove box for this procedure?

Is there a problem if there are too many spores in the syringe?


Edited by Shoegaze (07/21/15 04:23 AM)


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OfflineMatt87
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Re: Do I really need to make a mushroom print? [Re: Buck513]
    #21974241 - 07/21/15 05:05 AM (8 years, 6 months ago)

Quote:

Buck513 said:
Quote:

Matt87 said:
I'd just quit the syringe nonsense altogether and get into some g2g transfers. You have some experience now. Go for it.



He never said he was moving onto grain.



Just trying to give a nudge in that direction.


--------------------

Once you understand the way broadly, you see it in all things. -Musashi


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InvisibleMad Season
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Re: Do I really need to make a mushroom print? [Re: Matt87]
    #21975101 - 07/21/15 10:37 AM (8 years, 6 months ago)

All caps have a risk of contamination. They are fruited and opened up in open air after all. I seriously recommend getting into agar and LI.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisiblepaperbackwriter
Edward Lear


Registered: 03/31/14
Posts: 1,888
Re: Do I really need to make a mushroom print? [Re: Mad Season]
    #21975113 - 07/21/15 10:43 AM (8 years, 6 months ago)

Agar is fun and well worth learning.


--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley


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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
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Re: Do I really need to make a mushroom print? [Re: paperbackwriter]
    #21975169 - 07/21/15 11:01 AM (8 years, 6 months ago)

not much point in doing agar for pf-tek IMO.
spore syringes -> pf-tek works.

your suggestion works in theory shoegaze, but I wouldnt have the mushroom cap touching the jar,
try to suspend it,
and I wouldnt use that much spores in a syringe. 1 medium sized cube print easily makes 50 syringes.
more spores = more contam spores = bigger risk of fail.
less spores = still way more spores than needed, but way less contam spores = success :cookiemonster:


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InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: Do I really need to make a mushroom print? [Re: paperbackwriter]
    #21975170 - 07/21/15 11:02 AM (8 years, 6 months ago)

The only problem with too many spores in a syringe is the fact you'll be working with a lot more genetics, which is a bad thing


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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OfflineAero
Orea


Registered: 11/01/13
Posts: 2,253
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Re: Do I really need to make a mushroom print? [Re: Buck513]
    #21975292 - 07/21/15 11:36 AM (8 years, 6 months ago)

Quote:

Buck513 said:
Just make a syringe like everyone else.

1/5 of a print, or even 1/10 of a print is enough for a syringe.



more like 1 print is enough for like 100 syringes


--------------------
SPREAD THE SPORES



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InvisibleBuck513
Registered: 04/17/14
Posts: 5,682
Re: Do I really need to make a mushroom print? [Re: Aero]
    #21975379 - 07/21/15 12:05 PM (8 years, 6 months ago)

Your point? Everyone knows you don't need much.

I've yet to see someone make 100 syringes from a print. But you're right, that would still be enough.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
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Re: Do I really need to make a mushroom print? [Re: Buck513]
    #21975455 - 07/21/15 12:30 PM (8 years, 6 months ago)

agar is where it's at for sure. definately my next move


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineShoegaze
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Registered: 03/30/15
Posts: 5
Last seen: 8 years, 4 months
Re: Do I really need to make a mushroom print? [Re: tryptkaloids]
    #21976074 - 07/21/15 03:21 PM (8 years, 6 months ago)

Quote:

your suggestion works in theory shoegaze, but I wouldnt have the mushroom cap touching the jar,
try to suspend it,
and I wouldnt use that much spores in a syringe. 1 medium sized cube print easily makes 50 syringes.
more spores = more contam spores = bigger risk of fail.




I have a steel mesh to put on the jar so the shroom isn't touching it and the spores fall into the jar through it. I've also made a glove box to help keep contaminations out.

I figured if i put 100ml sterile water in the jar and shake it up with the spores in it, I can fill up 5 20ml syringes and just keep them in the fridge to inoculate the next 5 batches of 12 jars.

Is it still a silly idea or am I going to have to go down the spore print route? I'm just trying to keep things simple and cheap.


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InvisibleMad Season
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Re: Do I really need to make a mushroom print? [Re: Shoegaze]
    #21976118 - 07/21/15 03:32 PM (8 years, 6 months ago)

You should just get enough for 1 syringe and squirt it into like a litre of sterile water. Have a SHIP and draw shit ton of syringes, or just draw them when needed.

Or just print it into 1L of water :shrug: lol..


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (07/21/15 03:36 PM)


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OfflineShoegaze
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Re: Do I really need to make a mushroom print? [Re: Mad Season]
    #21976174 - 07/21/15 03:43 PM (8 years, 6 months ago)

Whats a SHIP?


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Invisibletahoe
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Re: Do I really need to make a mushroom print? [Re: OPB]
    #21976220 - 07/21/15 03:53 PM (8 years, 6 months ago)

Quote:

OPB said:
Just drop the mushroom cap in a shallow empty jar...close it...wait 24 hours...remove cap...add water...shake...suck into syringe.



Like opb said, this is one way to do it. Works well and you could make a hundred 10cc spore syringes from one print.

The op idea would work also but seems like an invite for contams. Both sound great in theory. But...
Contamination sneaks in wherever it can. You would want to wait the cap starts dropping heavy spores. Your screen to hold the cap must be sterile. It should work just fine. I would make a shit load of syringes at one time and then randomly test them with a jar.

One problem with spores dropping onto water is that they will want to float. Maybe add a few drops of jet dry to the water before sterilization. 

Going g2g starts getting into too much work. I still prefer to grow from spore syringes on certain species. I work with agar on others.

What I would seriously consider is multiplying store bought syringes.  Companies overload their syringes now. They do so to be visibly pleasing.  It does not increase germination time and barely increases colonization times.
I would sterilize,  or better yet, buy 100cc bottle of sterile water from ebay. Squirt your new store bought syringe into the bottle and bam, you multiplied your spore syringes


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineShoegaze
Stranger
Registered: 03/30/15
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Last seen: 8 years, 4 months
Re: Do I really need to make a mushroom print? [Re: Shoegaze]
    #21976250 - 07/21/15 04:02 PM (8 years, 6 months ago)

I was planning to add the water after the shrooms has dropped its spores into the jar.

Anyway, I'm looking into this spore printing business, maybe I'll just do that like everyone else. Seems like the tried and tested method.


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OfflineOPB
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Re: Do I really need to make a mushroom print? [Re: Shoegaze]
    #21977039 - 07/21/15 07:06 PM (8 years, 6 months ago)

There a million ways to do it. Just learn how the process works and how to avoid contams then try whatever you want. Experimenting is a quick way to learn a lot. Mistakes teach you more than success every time.


--------------------
"Wasn't that fun"


Edited by OPB (07/21/15 10:44 PM)


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Offlinetryptkaloids
Learner
I'm a teapot


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Re: Do I really need to make a mushroom print? [Re: OPB]
    #21977950 - 07/21/15 10:19 PM (8 years, 6 months ago)

:whathesaid:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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