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hiperhiper
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Registered: 06/14/15
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Last seen: 8 years, 3 months
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Mini mono tub incubation phase
#21973146 - 07/20/15 09:47 PM (8 years, 6 months ago) |
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Hey guys
First of all I just wanted to thank everyone for helping me as a noob on my first attempt to grow cubensis.
I'm using wheat berrie grain spawn, on a mini monotub with coco coir and vermiculite.
Basically I've moved the culture yesterday from the jars (with a little bacterial contamination) to the mini mono tub container. But I just realized that the container doesn't have a transparent lid. But I do have a larger container , so I still have some questions
1-How much time do I need to leave the mini mono tub closed until it colonized everything so I can be ready for fruiting?
2-I still don't have a humidity sensor (It haven't arrived from amazon), how do I control that? if it has condensation is it ok? do I need to spray water everyday?


3-Do I need to replace this lid or can I just open and let it fruit inside the larger container?
4-how can I improve conditions for mycelium and kill bacteria or other contaminants? is there any solution such as peroxide or gypsum that I can put on the top? should I leave it dry or increase gas exchange?
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Machiavelliavore
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Registered: 12/08/14
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Re: Mini mono tub incubation phase [Re: hiperhiper]
#21973314 - 07/20/15 10:21 PM (8 years, 6 months ago) |
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1. At least until you reach full colonization of the bulk substrate, preferably a little longer.
2. You don't need one. If it's properly adjusted you shouldn't need to spray water at all. Condensation should be a normal result of temperature variations.
3. You don't need to replace the lid at all. Light coming in from the sides should be sufficient. Def don't leave it inside the larger container when fruiting. If you're really concerned, you can cut pannels out in the lid and put some kind of clear plastic sheet (clear shower curtain?) over them.
4. There's not much you can do now. Improve your spawn quality in the future and make sure your bulk substrate is properly hydrated. Most contamination comes from bad spawn.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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jesuisravi
The Old Noob



Registered: 06/24/15
Posts: 260
Loc: Midwest USA
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Re: Mini mono tub incubation phase [Re: hiperhiper]
#21973352 - 07/20/15 10:30 PM (8 years, 6 months ago) |
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Quote:
hiperhiper said: Hey guys
First of all I just wanted to thank everyone for helping me as a noob on my first attempt to grow cubensis.
I'm using wheat berrie grain spawn, on a mini monotub with coco coir and vermiculite.
Basically I've moved the culture yesterday from the jars (with a little bacterial contamination) to the mini mono tub container. But I just realized that the container doesn't have a transparent lid. But I do have a larger container , so I still have some questions
1-How much time do I need to leave the mini mono tub closed until it colonized everything so I can be ready for fruiting?
2-I still don't have a humidity sensor (It haven't arrived from amazon), how do I control that? if it has condensation is it ok? do I need to spray water everyday?


3-Do I need to replace this lid or can I just open and let it fruit inside the larger container?
4-how can I improve conditions for mycelium and kill bacteria or other contaminants? is there any solution such as peroxide or gypsum that I can put on the top? should I leave it dry or increase gas exchange?
You say there was some bacterial contamination in the contents of the jars that you put into the monotub? I hope you didn't say that.
-------------------- Most of my beliefs I acquired from my father and from John Wayne, and anything that wasn't ultra tough and ultra cool was to me ultra embarrassing. In fact, I lived in a state of near continuous embarrassment, never measuring up to the ridiculous standards I had accepted without question, applied to a framework of expectations neither I nor anyone else could meet.--J C Amberchele almost nothing important that ever happens to you happens because you engineer it. Destiny has no beeper; destiny always leans trenchcoated out of an alley with some sort of 'psst' that you usually can't even hear because you're in such a rush to or from something important you've tried to engineer. ” ― David Foster Wallace, Infinite Jest
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hiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
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Re: Mini mono tub incubation phase [Re: jesuisravi]
#21973431 - 07/20/15 10:44 PM (8 years, 6 months ago) |
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Yes there was some
People said that it was ok to birth contaminated mushrooms with bacteria in monotub (it was not black, just a little bit of yellow)
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hiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
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Re: Mini mono tub incubation phase [Re: hiperhiper]
#21973440 - 07/20/15 10:46 PM (8 years, 6 months ago) |
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Duh I always feel confused, people tell me to do different things here
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Mini mono tub incubation phase [Re: hiperhiper]
#21973447 - 07/20/15 10:48 PM (8 years, 6 months ago) |
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Who said spawning contaminated grain is okay?
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Mini mono tub incubation phase [Re: Buck513]
#21973461 - 07/20/15 10:51 PM (8 years, 6 months ago) |
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I've gotten 4-5oz from 1 flush of a contaminated tub. But that doesn't mean I was successful. If I would have known a jar or two was contaminated, I wouldnt have spawned it.
Don't worry about the lid, light coming from the sides will be fine. Wait a week or so after spawning before you check your tub. Once the top of the sub is fully colonized just put it under fruiting conditions.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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hiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
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Re: Mini mono tub incubation phase [Re: Buck513]
#21973482 - 07/20/15 10:55 PM (8 years, 6 months ago) |
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I don't like to point fingers on people because they been helping me, but I'm really confused on what to do :/
Mad season in this link: http://www.shroomery.org/forums/showflat.php/Number/21956483#21956483
" And I told you it would be fine lol. Guess I didn't describe it well enough. You don't have to toss it. You can still spawn it. Bacteria may slow it down and increase chances of contamination, but honestly what noobie hasn't spawned a bacterial jar? I'd say I've done well over 100 bacterial jars.
Bulk has beneficial microbes and is pretty hard for bacteria to colonize. Unlike mycelium which tears right through it.
You can probably get away with no gypsum. I just think you should try to get it still.
If you get a contamination before a flush, blame this bacteria."
Captainpaps "Bacterial contaminations arent that big of an issue mush will still grow. Ive had tubs get trich in em and placed em outside and they did fine. Even with contams the fruits are safe to eat"
More people too
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Mini mono tub incubation phase [Re: hiperhiper]
#21973541 - 07/20/15 11:06 PM (8 years, 6 months ago) |
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What I gather from what they're saying is, you can get damn near anything to produce mushrooms. If you have a contaminated jar, yes you can still get a yield from it. But if you have more jars, or can avoid using the jar, then don't use contaminated spawn.
I just said what type of yield you can get from a bacteria sub, but that doesn't mean I'll advise people to knowingly spawn dirty grain. If its all you got, use it I guess. But be prepared to take it outside if the contamination gets really bad so you're not getting your grow room filthy.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
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Re: Mini mono tub incubation phase [Re: hiperhiper]
#21973562 - 07/20/15 11:10 PM (8 years, 6 months ago) |
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I got 28oz fresh off a single jar with a bacterial patch I scooped out before spawning.
I think it's fine to try, especially on a mini scale. We're talking about risk of 1$ of coir vs waiting another 1.5 months for mushrooms.
And if it trichs out, you can still do fine work with a few spores in your house. It's not like putting an Ebola patient in the bunk above you.
Oh I agree with Buck, definitely grow clean and dirty spawn separately if you know about the contamination and it's not uniformly distributed in every jar.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Buck513

Registered: 04/17/14
Posts: 5,682
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Quote:
Machiavelliavore said: I got 28oz fresh off a single jar with a bacterial patch I scooped out before spawning.

You got more than a wet pound from 1 jar of dirty spawn? That's almost 3 dry ounces.
You got skills, son.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
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Re: Mini mono tub incubation phase [Re: Buck513]
#21973669 - 07/20/15 11:35 PM (8 years, 6 months ago) |
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It had a typical flush curve to 20 oz, but I just keep dunking it, it keeps not contaminating and flushing out a few more O's at a time. Perhaps this is because MSG's run a bit dry and I carefuly remove the entire mushroom from the sub so as not to leave anything to contaminate. Thing's still going after nearly 2 months lol, covered in craters and spores. As I said I scooped out the patch of bacteria though, maybe .25-.5cup, so the spawn was basically clean.
Had a similar MS pan with clean spawn yield shit in comparison. Loved loved loved to abort the majority of cockteasingly sexy pinsets.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Buck513

Registered: 04/17/14
Posts: 5,682
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I think you mean, you scooped out the bacteria that you seen.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: Mini mono tub incubation phase [Re: Buck513]
#21973704 - 07/20/15 11:45 PM (8 years, 6 months ago) |
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Quote:
Buck513 said: I think you mean, you scooped out the bacteria that you seen.
I think you mean you think I mean I scooped out the bacteria that I saw.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Buck513

Registered: 04/17/14
Posts: 5,682
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I guess you have xray vision and is able to spot EVERY single grain that has a contaminant on it.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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hiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
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Re: Mini mono tub incubation phase [Re: Buck513]
#21973756 - 07/21/15 12:00 AM (8 years, 6 months ago) |
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Thanks for the clarification!
Can I use peroxide to help anyhow?
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Buck513
Registered: 04/17/14
Posts: 5,682
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Re: Mini mono tub incubation phase [Re: hiperhiper]
#21973789 - 07/21/15 12:11 AM (8 years, 6 months ago) |
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Peroxide is really only used for cobweb, so no.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
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Last seen: 3 months, 20 days
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Re: Mini mono tub incubation phase [Re: Buck513]
#21973796 - 07/21/15 12:14 AM (8 years, 6 months ago) |
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^ Just hope there was a small enough amount of bacterial spawn that it will be surrounded and isolated by mushroom mycellium so trich can't get to it.
I left the spawn in the sun when I was new to try and acheive ideal colonization temps (ya know, MUST MAKE GO FAST.) It got really hot and a small strip at the edge that was taking direct sunlight for hours refused to colonize (identical on 4 jars.) I'm assuming it was bacteria. The rest of the jar colonized completely.
Everyone but me must have magical bacteria vision when they say their spawn is clean. Where are you bacteria? Show yourselves!
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Buck513
Registered: 04/17/14
Posts: 5,682
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Saying you have clean spawn, and saying you took a handful of dirty spawn from a jar and NOW its clean spawn, are two completely different things.
Don't even attempt to compare the two.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
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Last seen: 3 months, 20 days
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Re: Mini mono tub incubation phase [Re: Buck513]
#21973878 - 07/21/15 12:44 AM (8 years, 6 months ago) |
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Clean spawn (from bacteria) is a matter of degrees. If my spawn went from 15% contaminated to .25% contaminated, that spawn went from being contaminated enough to be able to sink a grow to not contaminated enough to change much of anything. Usually it's not possible to separate the bacteria the way it was with my jars due to location and geometry. Also the triggering of early pinning and gimpy fruits is a bitch if it occurs.
Like I said, I still have the substrate and it's still not contaminating after 2mo and 3-4 dunks.
--------------------
I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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jesuisravi
The Old Noob



Registered: 06/24/15
Posts: 260
Loc: Midwest USA
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Re: Mini mono tub incubation phase [Re: Buck513]
#21973901 - 07/21/15 12:55 AM (8 years, 6 months ago) |
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I feel your pain.Your tub is contaminated and it is all you have to show for a month and a half of work and high hopes. Probably your best bet would be to send a private message to a Trusted Cultivator. Send a photo along with the message asking for advice on this issue. I am only repeating what I have heard a hundred times on this issue. But if people who have some experience are advising you to keep the tub, well, I guess it makes sense to keep it, for a while at least. If is starts smelling bad, then it won't be so hard to toss it. Who knows? Maybe the good myc will dominate the situation and you will come off with a harvest after all.
-------------------- Most of my beliefs I acquired from my father and from John Wayne, and anything that wasn't ultra tough and ultra cool was to me ultra embarrassing. In fact, I lived in a state of near continuous embarrassment, never measuring up to the ridiculous standards I had accepted without question, applied to a framework of expectations neither I nor anyone else could meet.--J C Amberchele almost nothing important that ever happens to you happens because you engineer it. Destiny has no beeper; destiny always leans trenchcoated out of an alley with some sort of 'psst' that you usually can't even hear because you're in such a rush to or from something important you've tried to engineer. ” ― David Foster Wallace, Infinite Jest
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hiperhiper
Stranger
Registered: 06/14/15
Posts: 82
Last seen: 8 years, 3 months
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Re: Mini mono tub incubation phase [Re: jesuisravi]
#21984504 - 07/23/15 12:26 PM (8 years, 6 months ago) |
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Do I need to vent or put water? It has been 4 days already
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