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amyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 10 years, 2 months
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brassinosteriods, in rape seed pollen
#2191753 - 12/19/03 09:09 PM (20 years, 3 months ago) |
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someone just ordered a bunch of rape seed, will this be a sutible source of rape seed pollen or more specificly brassinosteroids?
heres the study if u dont know about the wonders of brassinosteroids
-------------------- "A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty." -Al Einstein
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micro
bunbun has a gungun
Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: brassinosteriods, in rape seed pollen [Re: amyloid]
#2191959 - 12/19/03 10:37 PM (20 years, 3 months ago) |
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Maybe if you could extract it.... I don't know if the pure pollen would have any effect. There was a conversation here a while ago about this if you want to read it, once the search fuction is back up.
-- Micro
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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micro
bunbun has a gungun
Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: brassinosteriods, in rape seed pollen [Re: micro]
#2192093 - 12/19/03 11:52 PM (20 years, 3 months ago) |
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BTW -- pressure coocking it would probably destroy it.
-- Micro
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 22 hours
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Re: brassinosteriods, in rape seed pollen [Re: amyloid]
#2192156 - 12/20/03 12:58 AM (20 years, 3 months ago) |
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You will have to plant the rape seeds, grow them, harvest a few pounds of pollen and then extract a few milligrams of brassionosteroids..... Much work if you ask me.
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: brassinosteriods, in rape seed pollen [Re: amyloid]
#2192234 - 12/20/03 01:44 AM (20 years, 3 months ago) |
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I don't know the facts, but it sounds like someone is setting themselves up for rape....perhaps the name of the seed is very fitting in this case...perhaps not. Joshua
-------------------- The Shroomery Bookstore Great books for inquiring minds! "Life After Death is Saprophytic!"
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justsmurfy
little bluetwerp
Registered: 09/09/03
Posts: 68
Last seen: 20 years, 1 day
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Re: brassinosteriods, in rape seed pollen [Re: Joshua]
#2192743 - 12/20/03 11:02 AM (20 years, 3 months ago) |
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Rape seed (Brassica rapa) had a name change for marketing reasons... It's now usually refered to as canola. Yeah, 'rape oil' probably wouldn't go over quite as well. By the way, turnips are also Brassica rapa. Yummy! If properly prepared. -JustSmurfy
-------------------- Unsuitable for Human Consumption
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amyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 10 years, 2 months
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Re: brassinosteriods, in rape seed pollen [Re: Anno]
#2193042 - 12/20/03 02:32 PM (20 years, 3 months ago) |
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- Much work if you ask me.
have you tried it before?
and how complicated is this extraction?
-------------------- "A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty." -Al Einstein
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micro
bunbun has a gungun
Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: brassinosteriods, in rape seed pollen [Re: amyloid]
#2193191 - 12/20/03 04:06 PM (20 years, 3 months ago) |
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See:
http://www.shroomery.org/forums/showflat...=&fpart=all
Also, here's something:
HPLC Fractionation and Identification of BRs Cultured cells and medium were collected at approximately 54 h when the rate of tracheary element formation was approximately 5% and then stored at -80?C. The stored cells (total 63.4 g fresh weight) were sonicated and extracted three times with methanol. The medium (total 1,500 mL) was filtrated with a filter paper (qualitative filter paper No. 1, ADVANTEC, Higashiosaka, Osaka, Japan) and extracted three times with chloroform. After evaporation of both solvents in vacuo, the crude extract was partitioned two times between chloroform and water. The chloroform soluble fractions were subjected to silica gel chromatography (Sep-Pak Vac 10 g/35 cc for the elute derived from the cells, Sep-Pak Vac 1 g/6 cc for the elute derived from the medium; Sap-Pak Vac, Waters, Milford, MA). The column was subsequently eluted with chloroform, 2% (v/v) methanol in chloroform, and 7% (v/v) methanol in chloroform. Both the 7% (v/v) methanol fractions were subjected to octadecylsilane (ODS)-HPLC (Senshu Pak, ODS-1151-D, 4.6 ? 150 mm) at a flow rate of 1 mL min-1 with the solvents of 65% (v/v) acetonitrile. HPLC fractions were collected every 1 min. HPLC fractions corresponding to 3 g fresh weight cells and 500 mL medium were subjected to bioassay as described above.
(http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=64857)
-- Micro
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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mycofile
Pooh-Bah
Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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Re: brassinosteriods, in rape seed pollen [Re: justsmurfy]
#2194174 - 12/21/03 04:54 PM (20 years, 3 months ago) |
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Rape oil is nice and just plain considerate. Rapists who offer no lubrication are the lowest of the low.
-------------------- "From a certain point of view" -Jedi Master Obi Wan Kenobi PM me with any cultivation questions. I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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amyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 10 years, 2 months
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Re: brassinosteriods, in rape seed pollen [Re: micro]
#2194186 - 12/21/03 05:06 PM (20 years, 3 months ago) |
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oh well, it was only 80c/lb.. ill just make a rape seed cake
-------------------- "A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty." -Al Einstein
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mycofile
Pooh-Bah
Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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Re: brassinosteriods, in rape seed pollen [Re: amyloid]
#2196216 - 12/22/03 05:19 PM (20 years, 3 months ago) |
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I think a better use for rape seed would be as a supplement, use it like spawnmate. Just autoclave it dry first, then mix into bulk substrate at the time of spawning. Search for more info on spawnmate and it's substitutes if interested.
-------------------- "From a certain point of view" -Jedi Master Obi Wan Kenobi PM me with any cultivation questions. I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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Baby_Hitler
Errorist
Registered: 03/06/02
Posts: 27,635
Loc: To the limit!
Last seen: 11 minutes, 31 seconds
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Re: brassinosteriods, in rape seed pollen [Re: mycofile]
#2196437 - 12/22/03 07:13 PM (20 years, 3 months ago) |
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The spawnmate and amycel website are both hacked right now.
-------------------- "America: Fuck yeah!" -- Alexthegreat “Nothing can now be believed which is seen in a newspaper. Truth itself becomes suspicious by being put into that polluted vehicle. The real extent of this state of misinformation is known only to those who are in situations to confront facts within their knowledge with the lies of the day.” -- Thomas Jefferson The greatest sin of mankind is ignorance. The press takes [Trump] literally, but not seriously; his supporters take him seriously, but not literally. --Salena Zeto (9/23/16)
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 22 hours
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Re: brassinosteriods, in rape seed pollen [Re: Baby_Hitler]
#2196490 - 12/22/03 07:43 PM (20 years, 3 months ago) |
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Baby_Hitler
Errorist
Registered: 03/06/02
Posts: 27,635
Loc: To the limit!
Last seen: 11 minutes, 31 seconds
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Re: brassinosteriods, in rape seed pollen [Re: Anno]
#2196498 - 12/22/03 07:49 PM (20 years, 3 months ago) |
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Crazy crackers.
-------------------- "America: Fuck yeah!" -- Alexthegreat “Nothing can now be believed which is seen in a newspaper. Truth itself becomes suspicious by being put into that polluted vehicle. The real extent of this state of misinformation is known only to those who are in situations to confront facts within their knowledge with the lies of the day.” -- Thomas Jefferson The greatest sin of mankind is ignorance. The press takes [Trump] literally, but not seriously; his supporters take him seriously, but not literally. --Salena Zeto (9/23/16)
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amyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 10 years, 2 months
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Re: brassinosteriods, in rape seed pollen [Re: Baby_Hitler]
#2196754 - 12/22/03 09:36 PM (20 years, 3 months ago) |
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quick, before they patch that vuln lets order a million billion pounds of spawnmate!
-------------------- "A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty." -Al Einstein
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simplemachine
Manfly
Registered: 09/14/03
Posts: 1,981
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Re: brassinosteriods, in rape seed pollen [Re: amyloid]
#2197198 - 12/23/03 04:21 AM (20 years, 3 months ago) |
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what is spawnmate? Now that their sites fucked I have to ask you guys....
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 22 hours
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Re: brassinosteriods, in rape seed pollen [Re: simplemachine]
#2197542 - 12/23/03 10:16 AM (20 years, 3 months ago) |
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amyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 10 years, 2 months
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Re: brassinosteriods, in rape seed pollen [Re: Anno]
#2197711 - 12/23/03 12:01 PM (20 years, 3 months ago) |
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what is your opinion on spawnmate anno?
-------------------- "A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty." -Al Einstein
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