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InvisibleMad Season
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Re: Metabolites...? [Re: insanemike]
    #21974862 - 07/21/15 09:28 AM (8 years, 6 months ago)

There's a chance the syringe will have endospores, just like any other kind of spore. Bacterium, spores and endospores can get under the cap through us handling them, dust, or whatever. However in the end, whatever spores end up there, will have a good chance to be seen on agar :smile:.

Regardless, you got bacteria somewhere down the line op.


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Offlineoppsicle
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Re: Metabolites...? [Re: insanemike]
    #21974899 - 07/21/15 09:41 AM (8 years, 6 months ago)

Quote:

insanemike said:
Quote:

Inocuole said:
I don't think they'd be endospores if they were in a syringe, just... regular spores, or actual live bacteria.  Aren't endospores something that's left in organic material after they've already been contaminated long enough for a reproduction cycle to occur?  Maybe I have no idea..




You maybe right about the endospores or lack thereof.  I'm not really that well versed in those studies but when I stopped shooting up jars with spore syringes, bacteria problems took a dramatic turn for the better. After increasing pc times from 90 minutes to 120 minutes, the combination of the two changes, have really increased success rate.

Op, your first mistake was buying that over priced grow kit. If you put the hours equal to the dollars you spent on that kit into reading and learning how to cultivate, you would be a lot better off.





Ha like I said man, third grow ever, gonna do my own brf jars in the future.  You gotta get started somewhere! I don't know anyone else, my only teacher was google.  I don't regret buying from midwest, their grains are high quality and it was a really nice way to ease in without being overwhelmed with conflicting information. And frankly I just don't have much free time for lengthy prep like that.  Got more money than time these days. But I'm gonna do my best to squeeze it in :wink:


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OfflinePsilosoulful

Registered: 09/05/14
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Re: Metabolites...? [Re: oppsicle]
    #21974940 - 07/21/15 09:49 AM (8 years, 6 months ago)

Quote:

oppsicle said:
It's a cake of grains that happen to be in a bag. Regardless of proper terminology they are cake like objects. lol. but yeah they're 6 cups each so I just lost so many shrooms. I think I'll probably try it for posterity's sake.  Holy lame though. Never had a contam before, I wonder if my chamber slipped over 81 degrees at some point...



It's a grain bag lol. Cakes are the brf type in half pint jars. Anyway, you shouldn't have to use an incubation chamber during the colonization stage, room temp would be fine, anywhere between 70-78 is okay for all stages of mushroom growing. If the temp slipped past 81, it's no big deal, just make sure it doesn't exceed 86 or you could be stalling mycelial growth and allow bacteria to thrive.

And also, It would be a lot easier to start from scratch, then dealing with contams later on. It gets really frustrating when you're fruiting a tub and you see trich on one corner, and you have to decide whether or not to continue or toss out the tub you put soo much effort into. If you can get yourself a PC and a bag of grains, you can have multiple jars/bags going at the same time, and your success rate will most definitely increase.


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Offlineoppsicle
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Re: Metabolites...? [Re: Psilosoulful]
    #21975466 - 07/21/15 12:34 PM (8 years, 6 months ago)

Quote:

Psilosoulful said:
Quote:

oppsicle said:
It's a cake of grains that happen to be in a bag. Regardless of proper terminology they are cake like objects. lol. but yeah they're 6 cups each so I just lost so many shrooms. I think I'll probably try it for posterity's sake.  Holy lame though. Never had a contam before, I wonder if my chamber slipped over 81 degrees at some point...



It's a grain bag lol. Cakes are the brf type in half pint jars. Anyway, you shouldn't have to use an incubation chamber during the colonization stage, room temp would be fine, anywhere between 70-78 is okay for all stages of mushroom growing. If the temp slipped past 81, it's no big deal, just make sure it doesn't exceed 86 or you could be stalling mycelial growth and allow bacteria to thrive.

And also, It would be a lot easier to start from scratch, then dealing with contams later on. It gets really frustrating when you're fruiting a tub and you see trich on one corner, and you have to decide whether or not to continue or toss out the tub you put soo much effort into. If you can get yourself a PC and a bag of grains, you can have multiple jars/bags going at the same time, and your success rate will most definitely increase.





YEAH, ACCORDING TO YOUR FASCIST SYSTEM OF UNWAVERABLE TERMINOLOGY!! :laugh: He said only half jokingly to this entire website...lol. I think I'm going to start calling them pies instead of cakes. but then people have no idea what I'm talking about most of the time anyway. i digress...

I've never had a contam before and I want to see what happens. i wouldn't mind if you explained trich to me as i am unfamiliar.  there's not a chance of growing something poisonous or something is there? (other than the booms obvs)


--------------------
Be free, my little ones!

In my belly. Free in my belly.


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Offlinetryptkaloids
Learner
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Registered: 02/08/15
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Re: Metabolites...? [Re: oppsicle]
    #21975519 - 07/21/15 12:49 PM (8 years, 6 months ago)

this is where those precious hours of research we have all done on here comes in! google led me here and I've never gone anywhere else for mycology questions. the advanced search on this site is your best friend. use it. do the research. we won't spoonfeed you http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary&nbsp; http://www.shroomery.org/9423/Mycological-and-OMC-abbreviations&nbsp; these will help, also, learn the mushroom info section. a lot less conflicting knowledge there than the boards IMO go for it! learn the mushroom!


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Metabolites...? [Re: oppsicle]
    #21975575 - 07/21/15 01:02 PM (8 years, 6 months ago)

Quote:

oppsicle said:
Quote:

Psilosoulful said:
Quote:

oppsicle said:
It's a cake of grains that happen to be in a bag. Regardless of proper terminology they are cake like objects. lol. but yeah they're 6 cups each so I just lost so many shrooms. I think I'll probably try it for posterity's sake.  Holy lame though. Never had a contam before, I wonder if my chamber slipped over 81 degrees at some point...



It's a grain bag lol. Cakes are the brf type in half pint jars. Anyway, you shouldn't have to use an incubation chamber during the colonization stage, room temp would be fine, anywhere between 70-78 is okay for all stages of mushroom growing. If the temp slipped past 81, it's no big deal, just make sure it doesn't exceed 86 or you could be stalling mycelial growth and allow bacteria to thrive.

And also, It would be a lot easier to start from scratch, then dealing with contams later on. It gets really frustrating when you're fruiting a tub and you see trich on one corner, and you have to decide whether or not to continue or toss out the tub you put soo much effort into. If you can get yourself a PC and a bag of grains, you can have multiple jars/bags going at the same time, and your success rate will most definitely increase.





YEAH, ACCORDING TO YOUR FASCIST SYSTEM OF UNWAVERABLE TERMINOLOGY!! :laugh: He said only half jokingly to this entire website...lol. I think I'm going to start calling them pies instead of cakes. but then people have no idea what I'm talking about most of the time anyway. i digress...

I've never had a contam before and I want to see what happens. i wouldn't mind if you explained trich to me as i am unfamiliar.  there's not a chance of growing something poisonous or something is there? (other than the booms obvs)



Give this a quick read, it goes into detail about contaminants: http://www.shroomery.org/5276/What-are-common-contaminants-of-the-mushroom-culture

And, I would never eat a mushroom off a contaminated substrate, it's not worth a trip to the hospital. If you see it, toss it. That's my rule.


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OfflineKizzle
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Re: Metabolites...? [Re: Psilosoulful]
    #21975709 - 07/21/15 01:44 PM (8 years, 6 months ago)

No ones ever gone to the hospital from eating a mushroom off a contaminated substrate :lol:


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InvisibleMad Season
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Re: Metabolites...? [Re: Kizzle]
    #21975717 - 07/21/15 01:46 PM (8 years, 6 months ago)

:whathesaid: every mushroom picked in the wild is from a contaminated substrate.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflinePsilosoulful

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Re: Metabolites...? [Re: Mad Season]
    #21975729 - 07/21/15 01:50 PM (8 years, 6 months ago)

:themoreyouknow:


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Offlinetryptkaloids
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Re: Metabolites...? [Re: Psilosoulful]
    #21975735 - 07/21/15 01:52 PM (8 years, 6 months ago)

very good to know


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlineoppsicle
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Re: Metabolites...? [Re: tryptkaloids]
    #21975980 - 07/21/15 03:02 PM (8 years, 6 months ago)

aw man you guys. I love youse guys. let's all hang out sometime. heh.


--------------------
Be free, my little ones!

In my belly. Free in my belly.


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Metabolites...? [Re: oppsicle]
    #21976521 - 07/21/15 05:04 PM (8 years, 6 months ago)

Quote:

oppsicle said:
aw man you guys. I love youse guys. let's all hang out sometime. heh.



:highfive:


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Offlineoppsicle
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Re: Metabolites...? [Re: Psilosoulful]
    #22053242 - 08/06/15 11:58 AM (8 years, 5 months ago)

Just wanted to say I've gotten a fine crop from those bags.  The naked cakes got trich pretty quickly, but I think that was due to some trich on some previous cakes rather than from the bacteria.  And I still got a nice flush out of em :wink:


--------------------
Be free, my little ones!

In my belly. Free in my belly.


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