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moehd
Go into the light and shine



Registered: 09/14/14
Posts: 780
Last seen: 1 year, 2 months
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pf tek contam proof
#21965571 - 07/19/15 02:49 PM (8 years, 6 months ago) |
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i recently did 10 jars
i had problems in the past with contams so i did a lil research i baked the verm and brown rice flour for 20 min 450f and used purified water(reveres osmosis) also i super grinded the verm layer on top. is that over kill or are we trying to overkill
Edited by moehd (07/19/15 02:59 PM)
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Charleskun
Sir Charlington



Registered: 02/25/15
Posts: 207
Last seen: 2 years, 11 months
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Re: pf tek [Re: moehd]
#21965656 - 07/19/15 03:00 PM (8 years, 6 months ago) |
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You can easily boil the jars in a pot with a lid. You don't NEED a pc for pf tek but I've gotten away with boiling pf tek jars every time without contams. And don't grind the verm layer just put normal dry verm and you are good to go.
-------------------- All I see Is darkness
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moehd
Go into the light and shine



Registered: 09/14/14
Posts: 780
Last seen: 1 year, 2 months
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well in that case everything is a go but i did need to grind the verm on the top layer itll act as a better filter and coat thoroughly the entire top layer of the substrate
now the only way they can contam is from inoculation
or if they get smashed into a a thousand pieces
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 15 hours, 23 minutes
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Use an uncontaminated spore syringe. That's the most common reason for contamination with PF tek. Baking the verm and BRF and using purified water isn't going to help anything.
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moehd
Go into the light and shine



Registered: 09/14/14
Posts: 780
Last seen: 1 year, 2 months
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Re: pf tek [Re: Kizzle]
#21965737 - 07/19/15 03:11 PM (8 years, 6 months ago) |
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Quote:
Kizzle said: Use an uncontaminated spore syringe. That's the most common reason for contamination with PF tek. Baking the verm and BRF and using purified water isn't going to help anything.
it does it kills any mold spores that would be laying dormant inside
now ik as soon as they got out of the oven they were for sure coated with more spores but i like to think of it as gross decontamination
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 15 hours, 23 minutes
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Re: pf tek [Re: moehd]
#21965751 - 07/19/15 03:15 PM (8 years, 6 months ago) |
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Trust me, mold spores are not going to survive 90 minutes of steam sterilization even without pressure. If you're getting mold in your jars it's getting in them after you sterilize.
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moehd
Go into the light and shine



Registered: 09/14/14
Posts: 780
Last seen: 1 year, 2 months
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Re: pf tek [Re: Kizzle]
#21965760 - 07/19/15 03:18 PM (8 years, 6 months ago) |
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really well i shouldnt have invested in this pc then
every how to video had a pc
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 15 hours, 23 minutes
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Re: pf tek [Re: moehd]
#21965802 - 07/19/15 03:25 PM (8 years, 6 months ago) |
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A PC will come in handy if you move on to whole grains. PF tek can be done without one though.
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WindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 24 days
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Re: pf tek [Re: Kizzle]
#21966013 - 07/19/15 04:05 PM (8 years, 6 months ago) |
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I did my first with just a stock pot lined with lids to space the jars from the bottom. It worked out fine, I did however get contams but it was due to my shoddy sterile technique. It seems if your spore syringe is 'clean' and hasn't shown any reason for failure, then it would be sterile technique. I believe my failure came from working in open air in a high spore load area. Make a SAB, and sterilize the needle with a torch in between each jar. The SAB took my failure rate from 50% to 5% alone.
Edited by WindWisperer (07/19/15 04:06 PM)
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moehd
Go into the light and shine



Registered: 09/14/14
Posts: 780
Last seen: 1 year, 2 months
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i only failed one project and it was due to in exp but since it i used the tricks i listed in my first post to make it impossible to fail and to spread some ideas
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: pf tek [Re: Kizzle]
#21966225 - 07/19/15 05:03 PM (8 years, 6 months ago) |
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Quote:
Kizzle said: Use an uncontaminated spore syringe. That's the most common reason for contamination with PF tek. Baking the verm and BRF and using purified water isn't going to help anything.
Quote:
Kizzle said: Trust me, mold spores are not going to survive 90 minutes of steam sterilization even without pressure. If you're getting mold in your jars it's getting in them after you sterilize.
^ Bacteria are the only thing that could potentially survive the 90 minute steaming. Bad syringe, bad technique, or failure to follow teks are the main reasons for failure.
You don't need a PC for PF tek, and infact I'd encourage you not to get one. If you're ever making so many cakes that you need a PC, you shouldn't be making cakes anymore.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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moehd
Go into the light and shine



Registered: 09/14/14
Posts: 780
Last seen: 1 year, 2 months
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what r your guys opinion on shooting spores in the middle of the jar
why isnt it the norm
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: pf tek [Re: moehd]
#21966436 - 07/19/15 06:02 PM (8 years, 6 months ago) |
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Because then you cannot see what is happening. . .
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WindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 24 days
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Quote:
Pastywhyte said: Because then you cannot see what is happening. . .
I made this mistake when I was in a hurry with my WBS jars, only one has visible growth after 2 weeks : / I would highly suggest inoculating against the glass/plastic/whatever container you use with MS.
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Charleskun
Sir Charlington



Registered: 02/25/15
Posts: 207
Last seen: 2 years, 11 months
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Re: pf tek contam proof [Re: moehd]
#21966463 - 07/19/15 06:09 PM (8 years, 6 months ago) |
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The tek and the instructions in the pf tek are there for a reason. People get good results when they follow the tek.
-------------------- All I see Is darkness
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moehd
Go into the light and shine



Registered: 09/14/14
Posts: 780
Last seen: 1 year, 2 months
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im gonna do it on the sides and the middle it can only safe time imo
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moehd
Go into the light and shine



Registered: 09/14/14
Posts: 780
Last seen: 1 year, 2 months
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Re: pf tek contam proof [Re: moehd]
#21966475 - 07/19/15 06:12 PM (8 years, 6 months ago) |
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i was impatient my first grow now im happy to wait
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Charleskun
Sir Charlington



Registered: 02/25/15
Posts: 207
Last seen: 2 years, 11 months
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Re: pf tek contam proof [Re: moehd]
#21966500 - 07/19/15 06:20 PM (8 years, 6 months ago) |
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For sure dude. We aren't trying to roast we just want you to do good! Good luck!
-------------------- All I see Is darkness
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moehd
Go into the light and shine



Registered: 09/14/14
Posts: 780
Last seen: 1 year, 2 months
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