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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: Pressure cooking problems [Re: matsc]
#21968609 - 07/20/15 01:03 AM (8 years, 6 months ago) |
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My half pint PF's shrunk heftily in the PC (60min@15psi.) It was irrelevant to me since I had jars with a single SHIP instead of PF lids. They took like 2 months to colonize, but I inoculated with only a single point cause I was a n00b and scared that spreading out my spores would reduce the chance of germination. 4point noc would have probably cut it to like 2 weeks.
They still seemed somewhat puck-like and ultra dense. Perhaps a fine vermiculite problem. Cakes I made in PP5's recently in the PC have seemed better.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Pressure cooking problems [Re: matsc]
#21969790 - 07/20/15 08:50 AM (8 years, 6 months ago) |
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that's exactly what happened to about half of my PF jars, the top layer rained down on the sides and is now in the BRF layer, which sucks. fortunately they are colonizing and looks like they are not contaminated, yet.
by the way, how long should I pressure cook contaminated jars to kill the contam before I can empty it and use it again.
by the way azur, let me know when you get on, id like to run my checklist for my agar by you before I attempt doing it, I want something to work that im doing.
ALso azur, those jars that look good are the same ones, just right after I took them out of the PC, the other pictures are the same jars just 6 days later.
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matsc
Stranger



Registered: 09/17/12
Posts: 681
Loc: Arizona
Last seen: 3 months, 9 days
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I just fill a small bucket with some water, add a bit (ounce or two) of bleach, dunk it in and open it submerged. The jar then gets a hot water and dish soap treatment, and the puck gets to soak for 5-10 minutes before getting flushed.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Don't pc your contammed jars. Just take em outside and empty them. Rinse them out. Then take em inside and was with soap and water. Then shower.
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jesuisravi
The Old Noob



Registered: 06/24/15
Posts: 260
Loc: Midwest USA
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Re: Pressure cooking problems [Re: matsc]
#21969831 - 07/20/15 09:04 AM (8 years, 6 months ago) |
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Quote:
matsc said: I just fill a small bucket with some water, add a bit (ounce or two) of bleach, dunk it in and open it submerged. The jar then gets a hot water and dish soap treatment, and the puck gets to soak for 5-10 minutes before getting flushed.
What is the rationale behind the bleach and soap? If the puck is fully colonized, it shouldn't such heroic measures to protect it--or are you taking about pucks that are contaminated in some way?
-------------------- Most of my beliefs I acquired from my father and from John Wayne, and anything that wasn't ultra tough and ultra cool was to me ultra embarrassing. In fact, I lived in a state of near continuous embarrassment, never measuring up to the ridiculous standards I had accepted without question, applied to a framework of expectations neither I nor anyone else could meet.--J C Amberchele almost nothing important that ever happens to you happens because you engineer it. Destiny has no beeper; destiny always leans trenchcoated out of an alley with some sort of 'psst' that you usually can't even hear because you're in such a rush to or from something important you've tried to engineer. ” ― David Foster Wallace, Infinite Jest
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matsc
Stranger



Registered: 09/17/12
Posts: 681
Loc: Arizona
Last seen: 3 months, 9 days
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Re: Pressure cooking problems [Re: jesuisravi]
#21969858 - 07/20/15 09:15 AM (8 years, 6 months ago) |
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Correct, I want to kill off contamination so it doesnt spread evil spores around. The soap and water is just a normal "I want to clean these" type deal.
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Pressure cooking problems [Re: azur]
#21969896 - 07/20/15 09:24 AM (8 years, 6 months ago) |
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gotcha, I thought you were supposed to PC to kill the contam spores etc. I am just trying to be proactive and have an answer to the what if scenario.
the grains look ok for day 6?
I will be attempting the agar soon, can I PM you azur?
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Sure
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Pressure cooking problems [Re: azur]
#21970166 - 07/20/15 10:44 AM (8 years, 6 months ago) |
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I was going to start another round of grain jars today but am a little hesitant until I start to see some growth in my current grains, today is day 6 and im start to get a little discouraged. I followed the tek by the book and shit is still fucked up, seems like the grains are getting really dry as the days go by.
I just PM'd you Azur. For the MEA im going to use 10mg agar, 10mg LME, 500ml water simmering for like 10 minutes mixing till dissolved, enough for 10 plates I believe. After PC'ing for 15 minutes at 15PSI(that correct?)I think im supposed to wait for a certain temp to pour them but I forget what the exact temp is. I also have conflicting reports on whether to wrap the plates with saran wrap or not.
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Edited by firstTIMER420 (07/20/15 10:45 AM)
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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I use premixed agar from fungi perfecti. I pc at 15psi for 45 min. After i shut it off, i wait 2 hours before opening the pc and pouring the agar. Then wait another 2 hours to put spores to them. I do not wrap my petris with syran wrap. I just put them in ziploc bags.
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Pressure cooking problems [Re: azur]
#21970247 - 07/20/15 11:11 AM (8 years, 6 months ago) |
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when PCing, do I put as much water in the PC as the lvl of agar to prevent boil over? if not how much water do you place in the PC
Also, I am going to drill a hole in the cap, place a SFD in the cap, then put foil over cap before PCing.
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matsc
Stranger



Registered: 09/17/12
Posts: 681
Loc: Arizona
Last seen: 3 months, 9 days
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The way to prevent boil over with agar is to let the pressure come down very slowly. As long as the temperature drops below boiling before the pressure hits atmospheric, it shouldn't be a problem.
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Re: Pressure cooking problems [Re: matsc]
#21970502 - 07/20/15 12:13 PM (8 years, 6 months ago) |
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As long as you don't pop the weight off to release pressure or run cold water over your PC to cool it down you shouldn't have boil over. I set my AA921 in front of the running flowhood while it is cooling, I'm sure that helps accelerate the cool down a little bit. I've never had boil over.
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Pressure cooking problems [Re: Juiceh]
#21970743 - 07/20/15 01:30 PM (8 years, 6 months ago) |
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how much water do you put in your PC when using it for agar?
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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I use only a half inch of water for a 35 min 20psi run for agar in my AA921. I also elevate the bottle to be completely out of the water.
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firstTIMER420
Born the son of a sharecropper..



Registered: 05/08/15
Posts: 1,025
Loc: US, maybe?
Last seen: 6 years, 4 days
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Re: Pressure cooking problems [Re: Juiceh]
#21970817 - 07/20/15 01:48 PM (8 years, 6 months ago) |
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hmmm, I see people say they elevate their bottles I guess ill try that for my first time, seeing as how my PF jars turned out being only partially submerged.
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