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Offlinedigdog
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Liquid Culture Experiments
    #21963896 - 07/19/15 09:01 AM (8 years, 6 months ago)

Hello,

So I am posting this here because I cannot seem to post in the sub forum of agar and liquid cultures. Not complaining, simply explaining :wink:

So I have done some experiments with Reishi LC I procured from one of our vendors.

1.
I used the water from a 18 hour wheat grain soak and strained it through coffee filters to remove any residue. I added nothing to it and PC'd at 15psi for 90 minutes in a 1/2 pint jar. After it cooled I scraped small amounts of mycelium from one a petri dish (MDA) and dropped it into the jar. I shelved it and waited.

Result:


The mycelium grew thick across the top of the liquid and also fairly well into the liquid as well.

I have not yet inoculated anything with this liquid but will follow up with results when I do.

2. Same method as above but with the addition of karo syrup to water at 50:1 and a very small amount of gypsum (I eyeballed it so maybe a gram or 2, not very precise I know).

Result:



As you can see the mycelium grew in this liquid like gang busters.

I made 2 of these jars and used one of them to inoculate a pint jar of wheat grain.

Result (7 days post inoculation):



I did not take a picture but that LC really took off in that grain fast. 24 hours after I inoculated it had began to spread from any of the grain it had touched.

I will not say anything as to the correctness of the methods I used because these are simply experiments I am doing to gauge results however, I am confident that grain jar will do well on substrate and grow Reishi.

Admittedly these results are no great accomplishment because from what I have read Reishi mycelium is tough as nails and can withstand a lot of abuse. But then again if growing Reishi is my goal with these experiments well so far so good. Of course if I cannot get it to pin and then grow nice healthy mushrooms well.... well that is a good lesson as well.

Thoughts?


Edited by digdog (07/19/15 09:10 AM)


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Invisibleeatyualive
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Registered: 08/17/01
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Re: Liquid Culture Experiments [Re: digdog]
    #21963944 - 07/19/15 09:24 AM (8 years, 6 months ago)

were you using anything to break up the mycelial mat at the top of the lc? like a stir bar or stir plate?

growth looks good. keep it updated. :cool:


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Offlinedigdog
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Registered: 04/30/15
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Re: Liquid Culture Experiments [Re: eatyualive]
    #21964055 - 07/19/15 10:02 AM (8 years, 6 months ago)

Quote:

eatyualive said:
were you using anything to break up the mycelial mat at the top of the lc? like a stir bar or stir plate?




I put a small stone in the bottom of each jar. It broke up the mycelium in the first jar pretty well. Of course the first one I used did not have the matting like that jar. I still had problems with the needle clogging up because the largest gauge I have is 18. I am ordering some bigger needles for LC purposes soon. Eventually I will get a magnetic stir plate and bars though. :smile:


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