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mushroomnoob1981
Stranger

Registered: 06/15/15
Posts: 137
Last seen: 2 years, 7 months
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slow colonization of rye berry spawn bag
#21956653 - 07/17/15 03:14 PM (8 years, 6 months ago) |
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Hello,
I've done my first grow (PF tek with BRF) and the results came out fantastic. I've decided to try a bulk substrate grow. I bought a pre-steralized rye berry spawn bag and injected it with some B+ spores from a spore syringe (following the the instructions from included with the span bag to a "T"). I'm also a biologist so I'm familiar with sterile technique.
That was 2-3 weeks ago. I've been keeping the bag in the dark at 76 °F. That was about 2 weeks ago. I now the spores are good because I inoculated several PF BRF tubes as well and currently have tons of myc (some tubes are almost complete already).
There doesn't appear to be that much myc on the rye berries... by that I mean when I move the bag around a bit, I can spot like 1 or two berries that have some white stuff on a very, very small number of berries.
I have 4 main questions:
1-How long does a spawn bag take to colonize? 2-Could my moving the bag around be affecting the colonization process? 3-Do I need to invest in an incubation chamber to bring the temperature up to 86 °C? 4-Would it be a good idea to open the bag up and mix in some BRF/verm to kickstart the colonization process? I have access to an autoclave, so sterilization is no problem for me.
Thanks!
Edited by mushroomnoob1981 (07/17/15 03:14 PM)
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lovesquare
Love²

Registered: 06/04/15
Posts: 556
Last seen: 8 years, 4 months
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Quote:
mushroomnoob1981 said: 3-Do I need to invest in an incubation chamber to bring the temperature up to 86 °C?
76F is fine, 186F is way too hot.
-------------------- If you go down round the bend in the river, You're gonna find a few changes been going down there. If you go down to the gas-powered flatland, Where most of the people just think that they're free, Remember the peace that you had on the mountain, Come back to the love that you had here with me...
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: slow colonization of rye berry spawn bag [Re: lovesquare]
#21956753 - 07/17/15 03:47 PM (8 years, 6 months ago) |
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colonization depends on temp and genetics. but I think you meant germinate? A bag shouldn't be kept much over 70F. 86f and hot is ancient outdated news.
dont open the bag it must be kept sterile, you'd only make a mess trying to get sterilized brf into sterilized grains.
rather inoc some more brf cakes, and use them as spawn. pre-made stuff usually sucks!
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