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Offlinenk122
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Registered: 07/04/15
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Spore print to spore syringe no growth on rye seed issue
    #21953293 - 07/16/15 06:51 PM (8 years, 6 months ago)

I managed to put my own spore syringe together put a mature cubensis cap on a piece of aluminum foil for 24 hrs inside a tot box glove box i constructed.Sterilized some distilled water in pc for 30 mins at 15 psi i put a small amount of spores into a cleaned shotglass scraped some spores first then added the water sucked some water up and back down into shot glass twice to evenly distribute spores throughout the syringe.

Before all this the spore print was sitting in temps ranging anywhere from 70 degrees up to maybe 105 degrees F  for nearly 4 months wrapped up in the aluminum foil in a closet shelf. The spore syringe was shot up thru a self healing injection port on a quart sized jar with 1 cup of sterilized rye grass seed hydrated with 165 ml of water. i shook the syringe before shooting .5 ml of solution . FYI after i made the spore syringe it sat in the refrigerator for 2-3 days before use.

any ideas why i havent seen any growth is it possible the spore print was no vial to begin with im assumeing so and if so what is the proper method of preserving a print too keep its viability


Edited by nk122 (07/16/15 06:52 PM)


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OfflineBLINKfan420
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Registered: 07/06/12
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Re: Spore print to spore syringe no growth on rye seed issue [Re: nk122]
    #21953483 - 07/16/15 07:41 PM (8 years, 6 months ago)

How long has it been since you knocked up the grain? Give it 2 weeks maybe 3 before you call it a loss. 

BLiNK


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All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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OfflineDMoney718
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Registered: 06/05/15
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Last seen: 8 years, 6 months
Re: Spore print to spore syringe no growth on rye seed issue [Re: BLINKfan420]
    #21955462 - 07/17/15 10:14 AM (8 years, 6 months ago)

You used half a ml it will take a whileee lol just leave them and don't shake the jars at all until u see healthy myc big myc growths


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Offlinespacechildo
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Registered: 01/24/13
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Re: Spore print to spore syringe no growth on rye seed issue [Re: DMoney718]
    #21955586 - 07/17/15 10:50 AM (8 years, 6 months ago)

germination shouldn't take longer or shorter time depending on the amount of spores used.
they're still spores and need some time to germinate. I think 0,5mL is quite a lot to shoot into a grain jar!


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