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Offlineknomadic_niki
A mile high
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Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
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making a spore syringe
    #21952069 - 07/16/15 01:29 PM (8 years, 6 months ago)

okay, so i did some research about how to make a spore syringe but one subject wasn't brought up in the discussions: the spore prints aren't sterile. so how can you ensure that your spore syringe is clean unless you're growing and making prints in a sterile environment?


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InvisibleToadstool5
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Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
Re: making a spore syringe [Re: knomadic_niki]
    #21952114 - 07/16/15 01:40 PM (8 years, 6 months ago)

Your spore prints should have been made in a sterile environment and used in a sterile environment. I have made a decent amount of syringes from different isolates and have never had an issue, all of the prints were taken in front of a laminar hood. All syringes made in a SAB before the prints are even 6 months old. Always sterilize used syringes and water while following good aseptic procedure.

If its comprimised and somehow dirty the only option i know of is cleaning it up on agar or brf paste.

Stro's Cleaning and Isolating On Agar:
https://www.shroomery.org/forums/showflat.php/Number/18430998#18430998

You can swab spores fom the print onto the agar or put a drop of the MS solution fro the syringe on the agar. Isolate as soon as possible and continue until its clean :thumbup:


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

AMU Teks :mushroom2: Stro's Write Ups


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OfflineDarkhome
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Registered: 07/10/15
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Re: making a spore syringe [Re: Toadstool5] * 1
    #21952127 - 07/16/15 01:43 PM (8 years, 6 months ago)

:whathesaid:
Agar ....FTW!


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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Offlineknomadic_niki
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Re: making a spore syringe [Re: Darkhome]
    #21952134 - 07/16/15 01:45 PM (8 years, 6 months ago)

okay, that's exactly the info i was looking for. i'll take my next prints in front of the flow hood. but still, the caps aren't clean. do you swab them with alcohol or something?

i'll have to clean up my last print on agar. i wont bother making a syringe with it in that case. thanks!


--------------------
My trade list

In search of sporeless oyster cultures


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OfflineFreeWorldOrder
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Re: making a spore syringe [Re: knomadic_niki]
    #21952208 - 07/16/15 02:11 PM (8 years, 6 months ago)

I take my prints inside a new ziplock bag. Then when the spores drop I drop the cap from the bag holding the bag upside down so shit don't land in it. Then I put the bag (cracked open) in a clean tupperware with lid to dry.

When I am ready to make a syringe I crack open the bag (wiped down with iso) in a SAB and inject sterile water.

When I am ready to draw out the solution I wipe with iso and stab the bag and draw out. I hold the bag by one top corner so all the solution flows to one bottom corner. Then jab through the bag right above the level of solution. (Done in a SAB)

I simply draw up boiling water into syringe and squirt it out into the sink 3 times and keep the 4th draw for my sterilized water.. Flame the tip and replace cap. Allow to cool. Then flame tip before I jab the bag and draw out solution. Once I have my solution in the syringe I flame it again and replace cap.

If you have a big print you can make several syringes with it.

I've done this many times and so far never had a failure.

Most of my prints I do on foil because I mainly use agar now.
But if I want a syringe this is how I do it...:thumbup:


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"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

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Edited by FreeWorldOrder (07/16/15 02:15 PM)


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InvisibleToadstool5
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Registered: 01/22/15
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Re: making a spore syringe [Re: knomadic_niki]
    #21952211 - 07/16/15 02:13 PM (8 years, 6 months ago)

Normally they are swabbed in bleach, iso, or hydrogen peroxide but be careful to not overdo it with bleach (kills spores).

Most people also grow the mushrooms for printing in a separate, cleaner, FC or inside a bag inside the FC to help with how many air-bourne contams land on it.

The way basidiomytes launch spores from the sterigmata allows the cap to be elevated above the sterile foil in a sterile environment. By elevating the cap to where it isnt touching the foil and wiping with iso there is almost no chance of bacterial contam. In front of the laminar hood or in a SAB under a glass there is almost no chance of mold spores and without filth and standing water there is not much chance for viral infections.

It just takes some tweaking of the teks, vigilence, and ocassionally agar isolation :shrug:

I have heard of many people using the ziploc bag method like above, personally i pefer foil because it can typically be stored a little longer.

Nothing is ever perfectly sterile so you have to simply try your best and learn agar like the back of your hand.


--------------------
If you do not know where the mushroom products you are consuming are grown, think twice before eating them. :badshroom:
- Paul Stamets

AMU Teks :mushroom2: Stro's Write Ups


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Offlinespacechildo
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Re: making a spore syringe [Re: knomadic_niki]
    #21952541 - 07/16/15 03:44 PM (8 years, 6 months ago)

Quote:

knomadic_niki said:
okay, that's exactly the info i was looking for. i'll take my next prints in front of the flow hood. but still, the caps aren't clean. do you swab them with alcohol or something?

i'll have to clean up my last print on agar. i wont bother making a syringe with it in that case. thanks!




the best way to take the cleanest prints is from an invitro fruit.
the second best thing to do is to use minimal of spores into each syringe.
plenty of spores and less risk of picking up something nasty.

a couple drops in a grain jar will do, but of course agar is the only way to ensure clean growth.


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OfflineDarkhome
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Re: making a spore syringe [Re: FreeWorldOrder]
    #21957378 - 07/17/15 06:27 PM (8 years, 6 months ago)

Quote:

FreeWorldOrder said:
I take my prints inside a new ziplock bag. Then when the spores drop I drop the cap from the bag holding the bag upside down so shit don't land in it. Then I put the bag (cracked open) in a clean tupperware with lid to dry.

When I am ready to make a syringe I crack open the bag (wiped down with iso) in a SAB and inject sterile water.

When I am ready to draw out the solution I wipe with iso and stab the bag and draw out. I hold the bag by one top corner so all the solution flows to one bottom corner. Then jab through the bag right above the level of solution. (Done in a SAB)

I simply draw up boiling water into syringe and squirt it out into the sink 3 times and keep the 4th draw for my sterilized water.. Flame the tip and replace cap. Allow to cool. Then flame tip before I jab the bag and draw out solution. Once I have my solution in the syringe I flame it again and replace cap.

If you have a big print you can make several syringes with it.

I've done this many times and so far never had a failure.

Most of my prints I do on foil because I mainly use agar now.
But if I want a syringe this is how I do it...:thumbup:



Awesome Info...thanks bro... I'm going to try this
:takingnotes:


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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OfflineDarkhome
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Registered: 07/10/15
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Re: making a spore syringe [Re: spacechildo]
    #21957380 - 07/17/15 06:28 PM (8 years, 6 months ago)

Quote:

spacechildo said:
Quote:

knomadic_niki said:
okay, that's exactly the info i was looking for. i'll take my next prints in front of the flow hood. but still, the caps aren't clean. do you swab them with alcohol or something?

i'll have to clean up my last print on agar. i wont bother making a syringe with it in that case. thanks!




the best way to take the cleanest prints is from an invitro fruit.
the second best thing to do is to use minimal of spores into each syringe.
plenty of spores and less risk of picking up something nasty.

a couple drops in a grain jar will do, but of course agar is the only way to ensure clean growth.



As usual...great advice:bow2:


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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OfflineAjahn Don
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Registered: 07/05/15
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Re: making a spore syringe [Re: Darkhome]
    #21962034 - 07/18/15 07:40 PM (8 years, 6 months ago)

This forum just blows me away. I would have never thought of making a spore syringe like that. Just so creative. Bravo!

:thumbup:


--------------------
"He's not altogether dense, but he's not altogether there."


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OfflineMachiavelliavore
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Registered: 12/08/14
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Re: making a spore syringe [Re: Ajahn Don]
    #21962227 - 07/18/15 08:19 PM (8 years, 6 months ago)

Yup.  Transfer the invitro fruit to sterlized (pc) foil inside SAB.

I haven't had any much issue with using carefuly prepared open-air prints directly onto grains/pf.  Perhaps a miniscule amount of bacteria that was colonized over.  I try to get still-air conditions, then quickly grab the caps and put them onto foil in a sanitized container.  After 12-24 hr remove the cap and leave the lid slightly cracked to dry.

Agar is probably the lowest effort, most surefire way to get a clean MS inoculant.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlineknomadic_niki
A mile high
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Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
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Re: making a spore syringe [Re: Machiavelliavore]
    #21962259 - 07/18/15 08:26 PM (8 years, 6 months ago)

good stuff, thanks y'all. love to hear all the different ways people accomplish this


--------------------
My trade list

In search of sporeless oyster cultures


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