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Invisiblemrdux
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Registered: 06/05/04
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Agar to Grain PE Bags
    #21949561 - 07/15/15 09:57 PM (8 years, 6 months ago)

Healthy ropey MS PE agar to rye grain bags on 6-25 and then shaken on 7-07.  Have had issues w/ trich this year.  These bags smelled really good and mushroomy when I mixed/shook them last week. They recovered w/in 24/36 hours.  Any thoughts?







here's is a direct spore ms bag from 6/2:


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InvisibleMycophile
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Re: Agar to Grain PE Bags [Re: mrdux]
    #21949712 - 07/15/15 10:42 PM (8 years, 6 months ago)

Look good to me. maybe a chance, of "wetspot" in that uncolonized portion. from what I see thought, I would expect success! but hey, time will tell.

any thoughts on the next step?


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Invisiblemrdux
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Re: Agar to Grain PE Bags [Re: Mycophile]
    #21967400 - 07/19/15 09:34 PM (8 years, 6 months ago)

6 lbs of rye berry spawn to 10lbs of hm compost in 2 66qt monotubs on 7/19.  3 layers spawn to 3 layers compost.  the bags smelled good and crumbled easily and uniformly.

here's a monotub that was spawned on 7/4 to hpoo, cased on 7/14 w/ 50/50 and currently awaiting fruiting...  Same MS syringe batch. The bags pictured above are one transfer away from the original MS syringe.  The monotub comes from the only WBS bag out of 15 that grew out from MS syringe to rye berry bags on 4/02. The WBS bag colonized very quickly - but had quite few myc metabolites in evidence. I had just set up my flow hood, so I did a G2G w/ the WBS bag to some fresh Rye Berry bags.  Of the 5 bags, 2 had obvious trich contam and I tossed them. I'm looking forward to cloning a few from this tub! 



Still playing w/ the PE - after about 9 transfers I am here:


Same deal w/ some APE - knocked up 15 rye berry bags and had real aggressive growth in one.  Grabbed three rye grains and tossed them on agar before I tossed the bag.  I have 9 bags of agar to grain going w/ some really aggressive APE.  I can only hope that it fruits as well!




Edited by mrdux (07/19/15 09:57 PM)


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Invisiblemrdux
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Re: Agar to Grain PE Bags [Re: Mycophile]
    #21967569 - 07/19/15 10:10 PM (8 years, 6 months ago)

I should add that I had 3 bags of Mazatapec MS colonize w/ no distress on the 4/02 MS>WBS bag session.  I spawned them (6lbs) to 10 lbs of hpoo and was devastated when trich overtook the sub in a few days.

don't quite have the resources to do my own grains.  getting there.


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Invisiblemrdux
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Re: Agar to Grain PE Bags [Re: mrdux]
    #21991217 - 07/24/15 04:45 PM (8 years, 6 months ago)

Is this cobweb on the substrate?  one tub is looking normal but this one is all kinds of fuzzy.



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OfflinePsilosoulful

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Re: Agar to Grain PE Bags [Re: mrdux]
    #21991344 - 07/24/15 05:12 PM (8 years, 6 months ago)

Quote:

mrdux said:
Is this cobweb on the substrate?  one tub is looking normal but this one is all kinds of fuzzy.





Looks like the beginnings of some nasty trich. I would toss that man. :frown:


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Invisiblemrdux
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Re: Agar to Grain PE Bags [Re: Psilosoulful]
    #21991975 - 07/24/15 07:25 PM (8 years, 6 months ago)

it is outta here.  :mad2:

otoh, the 7/4 tub that was cased on 7/14 started pinning today.



Edited by mrdux (07/24/15 07:40 PM)


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OfflinePsilosoulful

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Re: Agar to Grain PE Bags [Re: mrdux]
    #21992103 - 07/24/15 07:44 PM (8 years, 6 months ago)

Quote:

mrdux said:
it is outta here.  :mad2:

otoh, the 7/4 tub that was cased on 7/14 started pinning today.





Congrats!!! :dancingbacon:


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Invisiblemrdux
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Re: Agar to Grain PE Bags [Re: Psilosoulful]
    #21997493 - 07/25/15 10:01 PM (8 years, 6 months ago)

looking good for the PE multispore that was started on wbs 4/2/15 - 1 bag out of 15 successfully consolidated.  Did a G2G to 5 2lb rye berry bags on 6/27/15.  As I noted above, tossed 2 of the 5 G2G bags w/ obvious trich.  Spawned ~5 lbs of rye berries to 10 lbs of hpoo on 7/4.  Cased w/ 5/8" (wide mouth jar ring) 50/50 on 7/14.  Pins popped on 7/24.  Lots of nice islands spread out over the entire tub.  Plenty of knots everywhere else.  I'm happy despite the extremely low MS syringe to bag ratio.  Ima guessing I should grab the big one soon and clone w/ agar?  Always amazed at the visible growth rate at this stage.



Edited by mrdux (07/25/15 10:08 PM)


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Invisiblemrdux
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Re: Agar to Grain PE Bags [Re: mrdux]
    #22021912 - 07/30/15 08:33 PM (8 years, 5 months ago)

not bad for ms.  i snapped this pic after i had started picking the lower part of the tub:



A nice pile:



went to case the other PE tub tonight and it has fungus gnat larva crawling all over.  I can't win some days.


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Offlineuninc4life2010
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Re: Agar to Grain PE Bags [Re: mrdux]
    #22021991 - 07/30/15 08:49 PM (8 years, 5 months ago)

Are you inoculating those bags in a glovebox or in front of a hood?  When you inoculate a bag, do you usually toss in one of the three sections of that dish?  I figure that a wedge wouldn't be enough to ensure quick colonization.  Whenever I've don't bags, I've always just inoculated through an injection port made out of duct tape on the bag.  I honestly have to say that my success rate is much lower with bags than with jars.  IDK if it is the moisture content, or that they aren't getting enough gas exchange.


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Invisiblemrdux
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Re: Agar to Grain PE Bags [Re: uninc4life2010]
    #22022066 - 07/30/15 09:07 PM (8 years, 5 months ago)

Quote:

uninc4life2010 said:
Are you inoculating those bags in a glovebox or in front of a hood?  When you inoculate a bag, do you usually toss in one of the three sections of that dish?  I figure that a wedge wouldn't be enough to ensure quick colonization.  Whenever I've don't bags, I've always just inoculated through an injection port made out of duct tape on the bag.  I honestly have to say that my success rate is much lower with bags than with jars.  IDK if it is the moisture content, or that they aren't getting enough gas exchange.




The bags were inoculated in front of a flow hood that is taller than the bags - which makes agar to grain and g2g pretty easy.  I think I can trace my trich problem back to one bag this spring.

I've been using one wedge per bag - works out real fast on 1lb bags - takes a bit longer on 2lb bags.  Usually mix up the bag at 10 - 14 days.


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Invisiblejesuisravi
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Re: Agar to Grain PE Bags [Re: mrdux]
    #22022462 - 07/30/15 10:41 PM (8 years, 5 months ago)

what was your source for manure? I mean where did you get it. The only thing available around here is the composted stuff from Home Depot and Lowes, which is part manure and part humus--i.e., "dirt".


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Most of my beliefs I acquired from my father and from John Wayne, and anything that wasn't ultra tough and ultra cool was to me ultra embarrassing. In fact, I lived in a state of near continuous embarrassment, never measuring up to the ridiculous standards I had accepted without question, applied to a framework of expectations neither I nor anyone else could meet.--J C Amberchele

almost nothing important that ever happens to you happens because you engineer it. Destiny has no beeper; destiny always leans trenchcoated out of an alley with some sort of 'psst' that you usually can't even hear because you're in such a rush to or from something important you've tried to engineer. ”
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Invisiblemaddchef
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Re: Agar to Grain PE Bags [Re: jesuisravi]
    #22022547 - 07/30/15 11:11 PM (8 years, 5 months ago)

Black kow and the composted manure do well as an additive but no more than 15% ish


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Invisiblejesuisravi
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Re: Agar to Grain PE Bags [Re: maddchef]
    #22023317 - 07/31/15 07:34 AM (8 years, 5 months ago)

The problem with the "manure" that the big box stores and nurseries are selling this time of year--late middle summer--is that it is about 90 percent dirt. Most of the packaging doesn't dare admit this but the ones who do, I have only seen one, say something like: Not less than 10% manure, not more than 90% humus or compost, i.e., mostly dirt. Earlier in the season Black Kow may be available. But it has all been snapped up long ago in May and June.


Edited by jesuisravi (07/31/15 07:35 AM)


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Offlineshroomyaxn
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Re: Agar to Grain PE Bags [Re: jesuisravi]
    #22023455 - 07/31/15 08:36 AM (8 years, 5 months ago)

I find the best aged horse manure from craigslist, fuck the big box stores shit...


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Invisiblemrdux
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Re: Agar to Grain PE Bags [Re: jesuisravi]
    #22035823 - 08/02/15 11:09 PM (8 years, 5 months ago)

best hpoo comes from local horse farms. They stack it for pick up by button mushroom farms, I pick up before the commercial folks do.


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