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InvisibleInocuole
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Re: Big Lots Agar dishes [Re: bodhisatta]
    #22033113 - 08/02/15 11:49 AM (8 years, 5 months ago)

Don't tell elastic I said this but... the tiger drop is terribly risky and screams bad practice.


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InvisiblebodhisattaMDiscordReddit
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Re: Big Lots Agar dishes [Re: Inocuole]
    #22033122 - 08/02/15 11:51 AM (8 years, 5 months ago)

I don't mind it in theory with pasty plates. But a dish :undecided:


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InvisibleInocuole
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Re: Big Lots Agar dishes [Re: bodhisatta]
    #22033149 - 08/02/15 12:00 PM (8 years, 5 months ago)

Popping it out of a dish seems a little fishy.  I'm pretty wary of doing it in a pasty plate too since you can't really see contams on a big ol piece of fully colonized agar.


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Coir-ca-cola
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OfflinetaGyo
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Re: Big Lots Agar dishes [Re: Inocuole]
    #22033162 - 08/02/15 12:03 PM (8 years, 5 months ago)

I do it all the time with my tri plates lol. I also had the big agar plates that I would cut in half to tiger drop. It's actually really easy in a dish.

Flame sterilize, reach in through the edge of the tri plate and push up, the piece will just pop off. Stab then drop.

If it's a full agar plate, flame sterilize, cut in half, go in from the edge, pop up and drop. I flame sterilize between agar wedges on a full plate because I'm crazy. On the tri-plate I pop them all off first and then stab and drop.

Wait, is that even tiger dropping?

:shrug:


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InvisibleInocuole
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Re: Big Lots Agar dishes [Re: taGyo]
    #22033202 - 08/02/15 12:11 PM (8 years, 5 months ago)

No, tiger dropping involves never touching anything and using the container it's in to pop it out.  There's no stab, no cut, no flame sterilize.


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OfflinetaGyo
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Re: Big Lots Agar dishes [Re: Inocuole]
    #22033238 - 08/02/15 12:17 PM (8 years, 5 months ago)

Then that's not what I do :lol:


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InvisibleInocuole
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Re: Big Lots Agar dishes [Re: taGyo]
    #22033245 - 08/02/15 12:19 PM (8 years, 5 months ago)

Sounds like you like to pop out big wedges, which is different but similar.


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Coir-ca-cola
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OfflinetaGyo
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Re: Big Lots Agar dishes [Re: Inocuole]
    #22033285 - 08/02/15 12:30 PM (8 years, 5 months ago)

Well tri-plates aren't big wedges.

When I had the full on agar dishes that I didn't like they were half the size of the plate, so yeah pretty big. But they always went into water for LI :thumbup:


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OfflineAjahn Don
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Re: Big Lots Agar dishes [Re: taGyo]
    #22033408 - 08/02/15 01:03 PM (8 years, 5 months ago)

Quote:

taGyo said:
they always went into water for LI :thumbup:




Not LI! Nooooo!!

:hotidea:


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OfflinetaGyo
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Re: Big Lots Agar dishes [Re: Ajahn Don]
    #22033409 - 08/02/15 01:03 PM (8 years, 5 months ago)

:hehehe:


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OfflineAjahn Don
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Re: Big Lots Agar dishes [Re: taGyo]
    #22035906 - 08/02/15 11:28 PM (8 years, 5 months ago)

:grin:


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OfflineAjahn Don
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Re: Big Lots Agar dishes [Re: Ajahn Don]
    #22041325 - 08/04/15 10:05 AM (8 years, 5 months ago)

I've got two Big Lots agar dishes growing out Golden Teachers on the left, and Texas yellow caps on the right.



What should I do with these. Both are showing nice growth. This is the second plate isolating these two from MS syringes. Sorry I can't get a better pic through the lid, but both are showing the fan formations now from the tomentose(?) growth. What should I do with them? Make a syringe and flood the plate, then suck up the water and inoculate grain jars? Or grow them further? I have BHTs fruiting and Mazas 75% colonized in BRF jars, probably my last PF teks, and 10 jars of oats starting to kick with KSSS ms, which I plan to do in a 20 qt mini. (I'm having such a good time.) Now, what next with these two plates? Where do I go from here?

Is this where second year students get stuck? Wondering what next? Or am I just an idiot, to go along with being a bald-headed old bastard?


:takingnotes:  :foreheadslap:  :biggrin:


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Offlineshroomyaxn
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Re: Big Lots Agar dishes [Re: Ajahn Don]
    #22041376 - 08/04/15 10:16 AM (8 years, 5 months ago)

Quote:

Ajahn Don said:
I've got two Big Lots agar dishes growing out Golden Teachers on the left, and Texas yellow caps on the right.







"This image has been deleted by the original poster"

Need a new image mate!


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OfflineAjahn Don
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Re: Big Lots Agar dishes [Re: shroomyaxn]
    #22041419 - 08/04/15 10:26 AM (8 years, 5 months ago)



Weird. It shows up for me.

Anyway, the Big Lots plates work well. There is a lot of condensation, however. Not sure what the remedy is for that.


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Offlineshroomyaxn
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Re: Big Lots Agar dishes [Re: Ajahn Don]
    #22041433 - 08/04/15 10:28 AM (8 years, 5 months ago)

Just realized I cannot see anyone's pics on any thread?! Must be just me then.  I'll look later on in the day.


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OfflinetaGyo
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Re: Big Lots Agar dishes [Re: shroomyaxn]
    #22041436 - 08/04/15 10:29 AM (8 years, 5 months ago)

It's site wide.

Give it some time.


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OfflineAjahn Don
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Re: Big Lots Agar dishes [Re: Ajahn Don]
    #22045978 - 08/05/15 07:20 AM (8 years, 5 months ago)

Quote:

Ajahn Don said:
I've got two Big Lots agar dishes growing out Golden Teachers on the left, and Texas yellow caps on the right.



What should I do with these. Both are showing nice growth. This is the second plate isolating these two from MS syringes. Sorry I can't get a better pic through the lid, but both are showing the fan formations now from the tomentose(?) growth. What should I do with them? Make a syringe and flood the plate, then suck up the water and inoculate grain jars? Or grow them further? I have BHTs fruiting and Mazas 75% colonized in BRF jars, probably my last PF teks, and 10 jars of oats starting to kick with KSSS ms, which I plan to do in a 20 qt mini. (I'm having such a good time.) Now, what next with these two plates? Where do I go from here?

Is this where second year students get stuck? Wondering what next? Or am I just an idiot, to go along with being a bald-headed old bastard?


:takingnotes:  :foreheadslap:  :biggrin:




C'mon, guys, I really need the benefit of your wisdom here. Too many questions at once? lol


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InvisibleMunchauzen
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Re: Big Lots Agar dishes [Re: Ajahn Don]
    #22046033 - 08/05/15 07:46 AM (8 years, 5 months ago)

Quote:

Ajahn Don said:
Make a syringe and flood the plate, then suck up the water and inoculate grain jars? Or grow them further? I




why not both? take 1-2 transfers from the plates then turn them into LI! I always transfer my LI plates, so that I retain a copy of that culture.


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OfflineAjahn Don
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Re: Big Lots Agar dishes [Re: Munchauzen]
    #22052402 - 08/06/15 09:08 AM (8 years, 5 months ago)

Since I got the right jars--wide mouth half pints--I'm going to take you advice and do both. I'll take a couple of chunks to fresh agar plates and then liquefy the rest on brf/verm/gyp. 3 jars each (since that's all I can do at once in my crappy PC) of golden teachers and TX yelow caps. Thanks for the help.

Don


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OfflineCenterfinger
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Re: Big Lots Agar dishes [Re: Ajahn Don]
    #22052570 - 08/06/15 09:52 AM (8 years, 5 months ago)

I picked up some of those dishes last night.  I haven't opened them yet but I think the lid is still cloudy rather than clear.  I may try to work with them upside down so I can clearly see what is going on.  I tried the small ziplock containers, but for me it's a problem that I have a hard time seeing what is going on with the growth.


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