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OfflineMysticPop
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Need urgent Help!! No growth and some grains turned green!!
    #21948392 - 07/15/15 05:07 PM (8 years, 6 months ago)

Hi All

I did grain inoculation using agar wedges. It has been almost 10 days and i barely see any signs of growth. However I do see that some grains have turned greenish and smell of mold. Should I toss all the grain jars? What are my other options??


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OfflinePsilosopherr
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948477 - 07/15/15 05:24 PM (8 years, 6 months ago)

Obviously you toss the green ones.

Read 'how frank gets shit done' linked in my sig to improve your sterile technique. His "how i do G2G in a SAB" might be particularly helpful.


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InvisibleSteveRogers
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948505 - 07/15/15 05:30 PM (8 years, 6 months ago)

Shitcan anything with green. Now.


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"General, I am loyal to nothing......except The Dream"


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Offlinespacechildo
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: SteveRogers]
    #21948529 - 07/15/15 05:34 PM (8 years, 6 months ago)

you should have done another transfer on agar as I told you to :tongue2:


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948534 - 07/15/15 05:35 PM (8 years, 6 months ago)

i know.. i lost patience.. had been doing agar transfers for past 2 months :frown:.. Now have to start all over again. Anyhow.. it gives me an experience and will improve my sterile technique..


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OfflinePsilosopherr
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948562 - 07/15/15 05:41 PM (8 years, 6 months ago)

Quote:

spacechildo said:
you should have done another transfer on agar as I told you to :tongue2:



was he transferring away from contams or something?


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: Psilosopherr]
    #21948597 - 07/15/15 05:48 PM (8 years, 6 months ago)

I was transferring from an overgrown agar plate.. And spacehildo advised me to do another transfer before doing grain inoculation.


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Offlinespacechildo
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: Psilosopherr]
    #21948627 - 07/15/15 05:53 PM (8 years, 6 months ago)

Quote:

rbalzer said:
Quote:

spacechildo said:
you should have done another transfer on agar as I told you to :tongue2:



was he transferring away from contams or something?




very likely, he had used very large wedges each time from fully grown plates.
some very clearly contaminated while others were just impossible to tell.

re your sab/fh problem you only need a sab dude, a fh is way overkill for now. and expensive.


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948832 - 07/15/15 06:37 PM (8 years, 6 months ago)

Is FH better than SAB in anyways ? Considering money is not an issue?


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Offlinespacechildo
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948840 - 07/15/15 06:39 PM (8 years, 6 months ago)

better at what? depends on what your situation is. they work differently but sorta the same.
not so sure you can work with contamed agar plates in front of a fh :shrug:


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948846 - 07/15/15 06:41 PM (8 years, 6 months ago)

i mean ..is it easier to pour agar plates, inoculate Agar plates, grain etc in front of FH than in SAB?


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948852 - 07/15/15 06:42 PM (8 years, 6 months ago)

Also for the Jars with mold, do i trash the grain with the jars or can i save the jars or the rings?


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Offlinespacechildo
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948872 - 07/15/15 06:47 PM (8 years, 6 months ago)

yeah a desk seems much easier to do the actual work at rather than inside a box on a desk.
but if your having problems working inside your box get a bigger box. never bothered me much :shrug:


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OfflineJohnDoeKENTUCKY
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948887 - 07/15/15 06:50 PM (8 years, 6 months ago)

Why not inoculate with spore syringe in to rye jars that have polyfil filter inside of small bathroom with a shit ton of Lysol sprayed? And then do the g2g the same way? I have been doing it this way for about a month and no contams. I didn't want to make a SAB because I don't see the point. You feel me or nah


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: JohnDoeKENTUCKY]
    #21948897 - 07/15/15 06:53 PM (8 years, 6 months ago)

What if the spore syringe is contaminated ?


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948934 - 07/15/15 07:01 PM (8 years, 6 months ago)

Also from what I have read, when u use agar you get to choose only rhyzophoric strains which have high chances of pinning than using the spore directly. Also I live in part where it is hard to get spore syringes.. I was able to get couple from RS but nobody else will ship here.. Unless I find someone here..


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948940 - 07/15/15 07:02 PM (8 years, 6 months ago)

Also for the Jars with mold, do i trash the grain with the jars or can i save the jars or the rings?


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OfflineJohnDoeKENTUCKY
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21949015 - 07/15/15 07:26 PM (8 years, 6 months ago)

Yea man just pressure cook after scrubbing it out. Mason jars are expensive as hell. Usually buy 100 at a time on Craigslist to save money.


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Offlinespacechildo
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21949018 - 07/15/15 07:27 PM (8 years, 6 months ago)

you must have been reading some really old shit. rhizo/tom doesnt matter for fruiting potential.
keep the jar. it gets sterilized next grain run anyway.

lysol is a surface disinfectant, and believing aerosols can cleanse the air well enough for our use
is something only pubbers do :nono:

I promise you a fuckton better yields just by using a SAB and not open air.

and mysticpop this is how you're supposed to do transfers, tiny pieces while myc is still growing.
http://www.mushroomvideos.com/Strain-Isolation


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OfflineJohnDoeKENTUCKY
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21949020 - 07/15/15 07:27 PM (8 years, 6 months ago)

Scrub them PC


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OfflineJohnDoeKENTUCKY
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21949034 - 07/15/15 07:32 PM (8 years, 6 months ago)

What do you mean by better yields? This is a noob question but can it actually effect mushroom fruit body yields if the rye or cake gets a small contam or would it just destroy entire jar/cake?
Thanks so much for your experience and knowledge by the way Spacechildo, you've made my life and many others lives better and you've probably saved thousands of jars from people that are noobs like me. Seriously thank you so much I truly appreciate your knowledge.
-John


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: JohnDoeKENTUCKY]
    #21949068 - 07/15/15 07:40 PM (8 years, 6 months ago)

Hey spacehildo,

So would u still recommend going the agar way or should I give direct inoculation to grain a try ?

Cheers
MysticPop


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Offlinespacechildo
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: JohnDoeKENTUCKY]
    #21949115 - 07/15/15 07:52 PM (8 years, 6 months ago)

Quote:

JohnDoeKENTUCKY said:
What do you mean by better yields? This is a noob question but can it actually effect mushroom fruit body yields if the rye or cake gets a small contam or would it just destroy entire jar/cake?




it depends on what you have, in most cases its toast, but fruits will still grow in the disaster of a tub.
bacteria can go unseen but seriously lower yields.

you should definitely continue using agar, just do it more like RR does it.


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21949237 - 07/15/15 08:26 PM (8 years, 6 months ago)

I was reading the Paul stamets book... Btw spacehildo I sent you a pm ..


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InvisibleMad Season
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21949471 - 07/15/15 09:35 PM (8 years, 6 months ago)

Seriously recommend you read my tek in my signature called improve your sterile techniques. It's a bit long, but I swear to god you'll come out knowing way more. I also seriously recommend no more overgrown plates. At least not until you've mastered transferring. Especially no overgrown plates on first transfer


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InvisibleGrey
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: Mad Season]
    #21949648 - 07/15/15 10:21 PM (8 years, 6 months ago)

Did you post pictures of the plates you made transfers to?

I only remember talking about the ones that had bacteria on them and then a later post with what seemed like the same plates after they'd been transferred from. We're those the plates used to inoculate these jars


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: Grey]
    #21949668 - 07/15/15 10:26 PM (8 years, 6 months ago)

yes. i used the following plates to inoculate the jars



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