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OfflineMysticPop
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Need urgent Help!! No growth and some grains turned green!!
    #21948392 - 07/15/15 05:07 PM (8 years, 6 months ago)

Hi All

I did grain inoculation using agar wedges. It has been almost 10 days and i barely see any signs of growth. However I do see that some grains have turned greenish and smell of mold. Should I toss all the grain jars? What are my other options??


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OfflinePsilosopherr
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948477 - 07/15/15 05:24 PM (8 years, 6 months ago)

Obviously you toss the green ones.

Read 'how frank gets shit done' linked in my sig to improve your sterile technique. His "how i do G2G in a SAB" might be particularly helpful.


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InvisibleSteveRogers
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948505 - 07/15/15 05:30 PM (8 years, 6 months ago)

Shitcan anything with green. Now.


--------------------
"General, I am loyal to nothing......except The Dream"


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Offlinespacechildo
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: SteveRogers]
    #21948529 - 07/15/15 05:34 PM (8 years, 6 months ago)

you should have done another transfer on agar as I told you to :tongue2:


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948534 - 07/15/15 05:35 PM (8 years, 6 months ago)

i know.. i lost patience.. had been doing agar transfers for past 2 months :frown:.. Now have to start all over again. Anyhow.. it gives me an experience and will improve my sterile technique..


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OfflinePsilosopherr
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948562 - 07/15/15 05:41 PM (8 years, 6 months ago)

Quote:

spacechildo said:
you should have done another transfer on agar as I told you to :tongue2:



was he transferring away from contams or something?


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: Psilosopherr]
    #21948597 - 07/15/15 05:48 PM (8 years, 6 months ago)

I was transferring from an overgrown agar plate.. And spacehildo advised me to do another transfer before doing grain inoculation.


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Offlinespacechildo
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: Psilosopherr]
    #21948627 - 07/15/15 05:53 PM (8 years, 6 months ago)

Quote:

rbalzer said:
Quote:

spacechildo said:
you should have done another transfer on agar as I told you to :tongue2:



was he transferring away from contams or something?




very likely, he had used very large wedges each time from fully grown plates.
some very clearly contaminated while others were just impossible to tell.

re your sab/fh problem you only need a sab dude, a fh is way overkill for now. and expensive.


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948832 - 07/15/15 06:37 PM (8 years, 6 months ago)

Is FH better than SAB in anyways ? Considering money is not an issue?


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Offlinespacechildo
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948840 - 07/15/15 06:39 PM (8 years, 6 months ago)

better at what? depends on what your situation is. they work differently but sorta the same.
not so sure you can work with contamed agar plates in front of a fh :shrug:


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948846 - 07/15/15 06:41 PM (8 years, 6 months ago)

i mean ..is it easier to pour agar plates, inoculate Agar plates, grain etc in front of FH than in SAB?


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948852 - 07/15/15 06:42 PM (8 years, 6 months ago)

Also for the Jars with mold, do i trash the grain with the jars or can i save the jars or the rings?


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Offlinespacechildo
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948872 - 07/15/15 06:47 PM (8 years, 6 months ago)

yeah a desk seems much easier to do the actual work at rather than inside a box on a desk.
but if your having problems working inside your box get a bigger box. never bothered me much :shrug:


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OfflineJohnDoeKENTUCKY
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21948887 - 07/15/15 06:50 PM (8 years, 6 months ago)

Why not inoculate with spore syringe in to rye jars that have polyfil filter inside of small bathroom with a shit ton of Lysol sprayed? And then do the g2g the same way? I have been doing it this way for about a month and no contams. I didn't want to make a SAB because I don't see the point. You feel me or nah


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: JohnDoeKENTUCKY]
    #21948897 - 07/15/15 06:53 PM (8 years, 6 months ago)

What if the spore syringe is contaminated ?


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948934 - 07/15/15 07:01 PM (8 years, 6 months ago)

Also from what I have read, when u use agar you get to choose only rhyzophoric strains which have high chances of pinning than using the spore directly. Also I live in part where it is hard to get spore syringes.. I was able to get couple from RS but nobody else will ship here.. Unless I find someone here..


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OfflineMysticPop
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21948940 - 07/15/15 07:02 PM (8 years, 6 months ago)

Also for the Jars with mold, do i trash the grain with the jars or can i save the jars or the rings?


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OfflineJohnDoeKENTUCKY
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21949015 - 07/15/15 07:26 PM (8 years, 6 months ago)

Yea man just pressure cook after scrubbing it out. Mason jars are expensive as hell. Usually buy 100 at a time on Craigslist to save money.


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Offlinespacechildo
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: spacechildo]
    #21949018 - 07/15/15 07:27 PM (8 years, 6 months ago)

you must have been reading some really old shit. rhizo/tom doesnt matter for fruiting potential.
keep the jar. it gets sterilized next grain run anyway.

lysol is a surface disinfectant, and believing aerosols can cleanse the air well enough for our use
is something only pubbers do :nono:

I promise you a fuckton better yields just by using a SAB and not open air.

and mysticpop this is how you're supposed to do transfers, tiny pieces while myc is still growing.
http://www.mushroomvideos.com/Strain-Isolation


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OfflineJohnDoeKENTUCKY
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Re: Need urgent Help!! No growth and some grains turned green!! [Re: MysticPop]
    #21949020 - 07/15/15 07:27 PM (8 years, 6 months ago)

Scrub them PC


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