Home | Community | Message Board

World Seed Supply
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom   North Spore Cultivation Supplies   Mushroom-Hut Mono Tub Substrate

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleKauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
Re: My invitro techinque [Re: Pastywhyte]
    #21953610 - 07/16/15 08:17 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Agreed. Invitro has its merits, I have done a few that way myself :thumbup:




I think, Invitro, there is absolutely nothing that comes close to how easy Sclerotia is … ultimate set and forget method.  I'm trying to perfect a combination of Violet's culturing method with RGS and Muda's grow bottles with a SGFC or if I get it going right, maybe a mini mono for 4-6 bottles.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


Extras: Filter Print Post Top
Invisiblejesuisravi
The Old Noob
Male User Gallery


Registered: 06/24/15
Posts: 260
Loc: Midwest USA Flag
Re: My invitro techinque [Re: spacechildo]
    #21953819 - 07/16/15 09:06 PM (8 years, 6 months ago)

Quote:

spacechildo said:
Quote:

KauaiOrca said:
but they involve a fruiting chamber.




Cron's Growing without a Fruiting chamber

teaches you very well how to read the mushrooms. read the whole thread for lots of good input on how to pull it off.




Thanks for posting this. A breakthrough! I would never had found this using the search engine here--should I blame the engine or the would be engineer? Anyway, an inspiring link. Double :thumbup: to you.


--------------------
Most of my beliefs I acquired from my father and from John Wayne, and anything that wasn't ultra tough and ultra cool was to me ultra embarrassing. In fact, I lived in a state of near continuous embarrassment, never measuring up to the ridiculous standards I had accepted without question, applied to a framework of expectations neither I nor anyone else could meet.--J C Amberchele

almost nothing important that ever happens to you happens because you engineer it. Destiny has no beeper; destiny always leans trenchcoated out of an alley with some sort of 'psst' that you usually can't even hear because you're in such a rush to or from something important you've tried to engineer. ”
― David Foster Wallace, Infinite Jest


Extras: Filter Print Post Top
InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: My invitro techinque [Re: durxo]
    #21954651 - 07/17/15 02:46 AM (8 years, 6 months ago)

Quote:

durxo said:
Quote:

blackout said:
I would skip the alcohol, it can harbour contams. Just cover the lid with foil, and only remove the foil just before you are inoculating, so the injection ports will be sterile.

It will also stop any water getting on your lid and into the containers through the filters. It looks like your lid might have a ridge running around it which will possibly make a pool in the centre if water drips off the top of the PC.

I would also recommend people to drill plastic on top of some wood, the plastic in the pics looks thick and not too brittle, others will crack. I usually drill a small hole and hold a lighter up to it, this smoothens out the edges and enlarges the hole and makes it stronger and smoother.




Yes you could too but the point of the isopropyl alcohol is to kill anything that might be in the needle and its really effective I dont care anymore where I do my prodecures, I do it in open air with the AC on, even in the kitchen where there is mold or with an open windows, and the alhocol doesnt make it trough the injection port, maybe some that might get caught inside the needle but never gave me any problems.

I keep my isopropyl alcohol inside a tincture bottle with a dropper.




So...are you one of those people that believe alcohol kills mold/bacteria efficiently?


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
Offlinedurxo
Stranger
 User Gallery


Registered: 01/16/14
Posts: 113
Last seen: 2 years, 1 month
Re: My invitro techinque [Re: Buck513]
    #21957222 - 07/17/15 05:53 PM (8 years, 6 months ago)

Quote:

Buck513 said:
Quote:

durxo said:
Quote:

blackout said:
I would skip the alcohol, it can harbour contams. Just cover the lid with foil, and only remove the foil just before you are inoculating, so the injection ports will be sterile.

It will also stop any water getting on your lid and into the containers through the filters. It looks like your lid might have a ridge running around it which will possibly make a pool in the centre if water drips off the top of the PC.

I would also recommend people to drill plastic on top of some wood, the plastic in the pics looks thick and not too brittle, others will crack. I usually drill a small hole and hold a lighter up to it, this smoothens out the edges and enlarges the hole and makes it stronger and smoother.




Yes you could too but the point of the isopropyl alcohol is to kill anything that might be in the needle and its really effective I dont care anymore where I do my prodecures, I do it in open air with the AC on, even in the kitchen where there is mold or with an open windows, and the alhocol doesnt make it trough the injection port, maybe some that might get caught inside the needle but never gave me any problems.

I keep my isopropyl alcohol inside a tincture bottle with a dropper.




So...are you one of those people that believe alcohol kills mold/bacteria efficiently?




Well at least for me it works really well, Ive never flame sterilized anything, I dont know if its my alcohol cause I have a laboratory grade 99% pure, so maybe is that but I dont believe it, Ive seen it many times, maybe Im wrong but I will keep doing it as long as it gives me good results.


--------------------

    “The psychotic drowns in the same waters in which the mystic swims with delight.”

    ― Joseph Campbell


Extras: Filter Print Post Top
Offlinedurxo
Stranger
 User Gallery


Registered: 01/16/14
Posts: 113
Last seen: 2 years, 1 month
Re: My invitro techinque [Re: KauaiOrca]
    #21957240 - 07/17/15 05:56 PM (8 years, 6 months ago)

I know, Im trying to cultivate truffles but I inoculated my bag with P. Mexicana like 6 weeks ago and nothing, I have the bag in a dark place at about 77-80 fahrenheit but I dont see any truffles, heres a picture.



--------------------

    “The psychotic drowns in the same waters in which the mystic swims with delight.”

    ― Joseph Campbell


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: My invitro techinque [Re: durxo]
    #21957246 - 07/17/15 05:58 PM (8 years, 6 months ago)

how do you know flame sterilizing wont give you even better results if you havent tried it? :derfase:


Extras: Filter Print Post Top
InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: My invitro techinque [Re: durxo]
    #21957249 - 07/17/15 05:58 PM (8 years, 6 months ago)

I mean....its a fact that alcohol doesn't kill all that it needs to.
Alcohol sanatizes, heat sterilizes. There is a tremendous difference.

Someone posted this awhile ago in another thread, the facts are right here why you shouldn't use alcohol like that

http://www.cdc.gov/hicpac/Disinfection_Sterilization/6_0disinfection.html


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: My invitro techinque [Re: durxo]
    #21957283 - 07/17/15 06:04 PM (8 years, 6 months ago)

Just because you get mushrooms to fruit, doesn't mean it was a success, or that it wasn't contaminated.
Many don't seem to realize that


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My invitro techinque [Re: durxo]
    #21957304 - 07/17/15 06:09 PM (8 years, 6 months ago)

Quote:

durxo said:
I know, Im trying to cultivate truffles but I inoculated my bag with P. Mexicana like 6 weeks ago and nothing, I have the bag in a dark place at about 77-80 fahrenheit but I dont see any truffles, heres a picture.






Bags always look sketchy but I think that looks bacterial. . .


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: My invitro techinque [Re: Pastywhyte]
    #21957318 - 07/17/15 06:12 PM (8 years, 6 months ago)

that's majorly bacterial.

but I have no exp with bacteria and stone formation so IDK if that's why :shrug:


Extras: Filter Print Post Top
InvisibleKauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
Re: My invitro techinque [Re: Pastywhyte]
    #21957528 - 07/17/15 07:12 PM (8 years, 6 months ago)

Quote:

Pastywhyte said:
Quote:

durxo said:
I know, Im trying to cultivate truffles but I inoculated my bag with P. Mexicana like 6 weeks ago and nothing, I have the bag in a dark place at about 77-80 fahrenheit but I dont see any truffles, heres a picture.






Bags always look sketchy but I think that looks bacterial. . .




I agree, that bag doesn't look good.  I got a Galindoi bag about that size going exactly 30 days ago and there are carmel and brown colored stones everywhere.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


Extras: Filter Print Post Top
InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: My invitro techinque [Re: KauaiOrca]
    #21957546 - 07/17/15 07:17 PM (8 years, 6 months ago)

:ilold:

I guess alcohol doesn't make such a great sterilizer after all :tongue2:


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
InvisibleGrey
⇜ ✯ ⇝
 User Gallery


Registered: 11/06/14
Posts: 6,223
Trusted Cultivator
Re: My invitro techinque [Re: KauaiOrca]
    #21957600 - 07/17/15 07:32 PM (8 years, 6 months ago)

Is possible you maybe didn't inject Mexicana into it, but maybe P.C. Cubensis Mexican?


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


Extras: Filter Print Post Top
Offlinedurxo
Stranger
 User Gallery


Registered: 01/16/14
Posts: 113
Last seen: 2 years, 1 month
Re: My invitro techinque [Re: Grey]
    #21958734 - 07/17/15 11:38 PM (8 years, 6 months ago)

Quote:

Grey said:
Is possible you maybe didn't inject Mexicana into it, but maybe P.C. Cubensis Mexican?




Yeah its possible cause I bought the loquid culture on internet.


--------------------

    “The psychotic drowns in the same waters in which the mystic swims with delight.”

    ― Joseph Campbell


Extras: Filter Print Post Top
Offlinedurxo
Stranger
 User Gallery


Registered: 01/16/14
Posts: 113
Last seen: 2 years, 1 month
Re: My invitro techinque [Re: Buck513]
    #21958740 - 07/17/15 11:40 PM (8 years, 6 months ago)

So you guys think it could be bacterial? I said alcohol does a good job cause when I cultivate shrooms everything looks fine, mycellium looks white and no strange odors.


--------------------

    “The psychotic drowns in the same waters in which the mystic swims with delight.”

    ― Joseph Campbell


Extras: Filter Print Post Top
InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: My invitro techinque [Re: durxo]
    #21958747 - 07/17/15 11:43 PM (8 years, 6 months ago)

I've managed to get more than 4-5oz from 1 flush on a contaminated sub.

Looks can be deceiving.


--------------------
Fail to plan and you plan to fail.

Enter the Ban Lottery


Extras: Filter Print Post Top
InvisibleGrey
⇜ ✯ ⇝
 User Gallery


Registered: 11/06/14
Posts: 6,223
Trusted Cultivator
Re: My invitro techinque [Re: durxo]
    #21958756 - 07/17/15 11:45 PM (8 years, 6 months ago)

To me, it looks like overcolonized grains by cube mycelium. If it's a 100% trim the bag and case the grains.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


Extras: Filter Print Post Top
Offlinedurxo
Stranger
 User Gallery


Registered: 01/16/14
Posts: 113
Last seen: 2 years, 1 month
Re: My invitro techinque [Re: Grey]
    #21961311 - 07/18/15 04:40 PM (8 years, 6 months ago)

Yeah I also tought it could be a cube strain and not P. Mexicana, I will do that to see what kind of fruit grows from it, thanks guys.


--------------------

    “The psychotic drowns in the same waters in which the mystic swims with delight.”

    ― Joseph Campbell


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: My invitro techinque [Re: durxo]
    #21961370 - 07/18/15 04:52 PM (8 years, 6 months ago)

Uh yeah that bag is extremely bacterial. That thick white look, and the wet grains.. i can't believe all you do is just use alcohol. Try using just alcohol on your scalpel for agar. I used to and it doesn't work. At all. Then I bought a blow torch. What a difference!

Oh BTW lab grade 99% alcohol is actually worse than 70% alcohol. Pure alcohol is nowhere near as good at sanitizing.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Offlinedurxo
Stranger
 User Gallery


Registered: 01/16/14
Posts: 113
Last seen: 2 years, 1 month
Re: My invitro techinque [Re: Mad Season]
    #21961555 - 07/18/15 05:41 PM (8 years, 6 months ago)

Ok next time I will flame sterilize


--------------------

    “The psychotic drowns in the same waters in which the mystic swims with delight.”

    ― Joseph Campbell


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom   North Spore Cultivation Supplies   Mushroom-Hut Mono Tub Substrate


Similar ThreadsPosterViewsRepliesLast post
* invitro dunk for sclerotia Yossarian22 608 8 03/29/08 06:32 PM
by Gastronomicus
* Best strain for Invitro?
( 1 2 3 all )
Syriss162 7,216 40 08/21/06 11:30 AM
by Capless
* myctopia's invitro double end casing... rohitabong 1,059 6 08/04/03 10:52 PM
by Magash
* Whats going on with my invitro bags? Realnuggetz 1,445 18 07/31/08 02:03 AM
by Realnuggetz
* Invitro spawn bag technique ozzyozzyozzy 2,672 4 06/12/14 04:26 AM
by flyingfurther
* invitro grow questions userx 380 4 03/02/18 04:11 PM
by nube424
* Inject poo myco bag bigshroom 1,690 11 09/12/06 02:14 PM
by monstermitch
* Invitro Printing? navyseals101 1,037 8 01/16/06 02:25 AM
by hawksapprentice

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,379 topic views. 31 members, 203 guests and 24 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.011 seconds on 14 queries.