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durxo
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My invitro techinque
#21943118 - 07/14/15 05:47 PM (8 years, 6 months ago) |
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Hi guys I want to share with you my in vitro cultivation method cause its really easy, maintenance free and contam free.
First I drill four holes into the top and two on the sides of an hermetic rubbermaid container with a 1/4" bit



Then I put two autohealing rubber injection ports in the top


Then I put micropore tape in the other four holes.


Then I fill it with BRF-vermiculae mixture (the same as PF Tek's), pressure cook the container, let it cool, then put a drop of isopropyl alcohol over the injection ports and inject it with contam-free liquid culture or spore syringe passing the needle through the alcohol while injecting.
Finally I put the container in a room with indirect sunlight and leave it there until it is ready to harvest.
This is the final result (one jar).






You can even harvest them some days after the caps opened and they will not spoil.
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“The psychotic drowns in the same waters in which the mystic swims with delight.” ― Joseph Campbell
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
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Re: My invitro techinque [Re: durxo]
#21943190 - 07/14/15 06:01 PM (8 years, 6 months ago) |
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Cool man.
Do you inoculate in open air, or in a SAB?
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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AquaticPuzzle
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Re: My invitro techinque [Re: durxo]
#21943306 - 07/14/15 06:25 PM (8 years, 6 months ago) |
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durxo
Stranger



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Re: My invitro techinque [Re: Grey]
#21944983 - 07/15/15 01:43 AM (8 years, 6 months ago) |
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Quote:
Grey said: Cool man.
Do you inoculate in open air, or in a SAB?
I inoculate in open air just passing the needle through the isopropyl alcohol and never had any contaminations.
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“The psychotic drowns in the same waters in which the mystic swims with delight.” ― Joseph Campbell
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Ajahn Don
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Re: My invitro techinque [Re: durxo]
#21946414 - 07/15/15 08:54 AM (8 years, 6 months ago) |
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This is worth investigation, imho. What a boon it would be for someone who just wants a few for the occasional flight. Neat. Thumbs up for the idea.
-------------------- "He's not altogether dense, but he's not altogether there."
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blackout


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Re: My invitro techinque [Re: Ajahn Don]
#21947384 - 07/15/15 01:26 PM (8 years, 6 months ago) |
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I would skip the alcohol, it can harbour contams. Just cover the lid with foil, and only remove the foil just before you are inoculating, so the injection ports will be sterile.
It will also stop any water getting on your lid and into the containers through the filters. It looks like your lid might have a ridge running around it which will possibly make a pool in the centre if water drips off the top of the PC.
I would also recommend people to drill plastic on top of some wood, the plastic in the pics looks thick and not too brittle, others will crack. I usually drill a small hole and hold a lighter up to it, this smoothens out the edges and enlarges the hole and makes it stronger and smoother.
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durxo
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Re: My invitro techinque [Re: blackout]
#21947853 - 07/15/15 03:09 PM (8 years, 6 months ago) |
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Quote:
blackout said: I would skip the alcohol, it can harbour contams. Just cover the lid with foil, and only remove the foil just before you are inoculating, so the injection ports will be sterile.
It will also stop any water getting on your lid and into the containers through the filters. It looks like your lid might have a ridge running around it which will possibly make a pool in the centre if water drips off the top of the PC.
I would also recommend people to drill plastic on top of some wood, the plastic in the pics looks thick and not too brittle, others will crack. I usually drill a small hole and hold a lighter up to it, this smoothens out the edges and enlarges the hole and makes it stronger and smoother.
Yes you could too but the point of the isopropyl alcohol is to kill anything that might be in the needle and its really effective I dont care anymore where I do my prodecures, I do it in open air with the AC on, even in the kitchen where there is mold or with an open windows, and the alhocol doesnt make it trough the injection port, maybe some that might get caught inside the needle but never gave me any problems.
I keep my isopropyl alcohol inside a tincture bottle with a dropper.
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“The psychotic drowns in the same waters in which the mystic swims with delight.” ― Joseph Campbell
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Darkhome
•Shaman•N•Training•



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Re: My invitro techinque [Re: durxo]
#21948168 - 07/15/15 04:12 PM (8 years, 6 months ago) |
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Nice Cap bro...
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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durxo
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Re: My invitro techinque [Re: Darkhome]
#21952506 - 07/16/15 03:38 PM (8 years, 6 months ago) |
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Quote:
Darkhome said: Nice Cap bro...
Thx man, nice signature haha
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“The psychotic drowns in the same waters in which the mystic swims with delight.” ― Joseph Campbell
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funkmastakush
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Re: My invitro techinque [Re: durxo]
#21952606 - 07/16/15 03:56 PM (8 years, 6 months ago) |
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How long did it take you from injection to harvest? Did you ever change its environment?
-------------------- “If the words ‘life, liberty, and the pursuit of happiness’ don’t include the right to experiment with your own consciousness, then the Declaration of Independence isn’t worth the hemp it was written on.” -Terence McKenna "Patience you must have my young Padawan" -Yoda
 
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KauaiOrca
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Registered: 08/12/08
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I find grows like this absolutely fascinating because obviously, a couple of very small holes with micropore tape on them gives enough FAE for healthy mushrooms to grow. Contrast that with a SGFC, fanning, misting to get the FAE and humidity right. I mean seriously, how much "fresh air" can be getting into that little fruiting chamber?
How much effort, testing, trial and error does it take you to find a strain or culture that thrives with this method or do they all work equally well.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
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durxo
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Quote:
funkmastakush said: How long did it take you from injection to harvest? Did you ever change its environment?
It took me like 2 weeks to make the liquid culture, then from when I injected the LC into the jar it took like 2-3 weeks in a dark place to fully colonize, then 1-2 weeks to harvest the mushrooms so it was total less that 1 and a half months from injection to harvest.
I only changed the environment once by getting it out of the dark room into a room with many windos over a cabine for indirect sunlight, I will upload the picture tomorrow.
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“The psychotic drowns in the same waters in which the mystic swims with delight.” ― Joseph Campbell
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durxo
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Re: My invitro techinque [Re: KauaiOrca]
#21953016 - 07/16/15 05:44 PM (8 years, 6 months ago) |
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Quote:
KauaiOrca said: I find grows like this absolutely fascinating because obviously, a couple of very small holes with micropore tape on them gives enough FAE for healthy mushrooms to grow. Contrast that with a SGFC, fanning, misting to get the FAE and humidity right. I mean seriously, how much "fresh air" can be getting into that little fruiting chamber?
How much effort, testing, trial and error does it take you to find a strain or culture that thrives with this method or do they all work equally well.
Well actually this was the first try.
One day I left a colonized jar in a table in the kitchen and a month later I noticed it had mushrooms in it between the cake and the glass so I decided to do this in a bigger jar with enough space for the mushrooms to grow.
I was having some contamination problems on my fruiting chamber so I thought it would be easier this way cause you pressure cook the entire thing and never open the jar until its ready to harvest.
The first jar I left in the kitchen had two 1/4" holes in the top but thisone is bigger so I added 2 more holes at the middle of the jar to let a little more FAE, I just left it over a cabinet in the kithen and some weeks later I noticed it was full of mushrooms =)
I will continue experimenting with different variables to see what happens and I will post my results.
By the way I used Psilocybe Cubensis B+ but since all the cubes have the same growing conditions I assume you can do this with any cube strain.
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“The psychotic drowns in the same waters in which the mystic swims with delight.” ― Joseph Campbell
Edited by durxo (07/16/15 05:47 PM)
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KauaiOrca
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Registered: 08/12/08
Posts: 3,131
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Re: My invitro techinque [Re: durxo] 1
#21953032 - 07/16/15 05:49 PM (8 years, 6 months ago) |
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Quote:
durxo said:
Quote:
KauaiOrca said: I find grows like this absolutely fascinating because obviously, a couple of very small holes with micropore tape on them gives enough FAE for healthy mushrooms to grow. Contrast that with a SGFC, fanning, misting to get the FAE and humidity right. I mean seriously, how much "fresh air" can be getting into that little fruiting chamber?
How much effort, testing, trial and error does it take you to find a strain or culture that thrives with this method or do they all work equally well.
Well actually this was the first try.
One day I left a colonized jar in a table in the kitchen and a month later I noticed it had mushrooms in it between the cake and the glass so I decided to do this in a bigger jar with enough space for the mushrooms to grow.
I was having some contamination problems on my fruiting chamber so I thought it would be easier this way cause you pressure cook the entire thing and never open the jar until its ready to harvest.
The first jar I left in the kitchen had two 1/4" holes in the top but thisone is bigger so I added 2 more holes at the middle of the jar to let a little more FAE, I just left it over a cabinet in the kithen and some weeks later I noticed it was full of mushrooms =)
I will continue experimenting with different variables to see what happens and I will post my results.
I'm into invitro methods as well and really have no desire for big tubs of bulk grows and thousands of mushrooms. I'm going to post three really good threads for you on this that you'll get a lot out of. Especially Violet's V-Tek … She's got this method totally dialed in. The Muda Bottle look like they could yield a lot but they involve a fruiting chamber.
http://www.shroomery.org/forums/showflat.php/Number/19035262#19035262
Sclerotia (magic truffles) is totally invitro and this guy is the wizard of sclerotia http://www.shroomery.org/forums/showflat.php/Number/13431948/page/1
http://www.shroomery.org/forums/showflat.php/Number/21168958#21168958
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
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durxo
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Re: My invitro techinque [Re: KauaiOrca]
#21953141 - 07/16/15 06:17 PM (8 years, 6 months ago) |
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Quote:
KauaiOrca said:
I'm into invitro methods as well and really have no desire for big tubs of bulk grows and thousands of mushrooms. I'm going to post three really good threads for you on this that you'll get a lot out of. Especially Violet's V-Tek … She's got this method totally dialed in. The Muda Bottle look like they could yield a lot but they involve a fruiting chamber.
http://www.shroomery.org/forums/showflat.php/Number/19035262#19035262
Sclerotia (magic truffles) is totally invitro and this guy is the wizard of sclerotia http://www.shroomery.org/forums/showflat.php/Number/13431948/page/1
http://www.shroomery.org/forums/showflat.php/Number/21168958#21168958
Wow thx man I will check it out, I was wondering if I could use brown rice as substrate cause I cant find RGS where I live and theyre a little expensive on internet but they use rice in your link so I will try it.
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“The psychotic drowns in the same waters in which the mystic swims with delight.” ― Joseph Campbell
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spacechildo
proletarians rise up



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Re: My invitro techinque [Re: KauaiOrca]
#21953191 - 07/16/15 06:27 PM (8 years, 6 months ago) |
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Quote:
KauaiOrca said: but they involve a fruiting chamber.
Cron's Growing without a Fruiting chamber
teaches you very well how to read the mushrooms. read the whole thread for lots of good input on how to pull it off.
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KauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
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Re: My invitro techinque [Re: durxo]
#21953444 - 07/16/15 07:31 PM (8 years, 6 months ago) |
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Quote:
durxo said:
Quote:
KauaiOrca said:
I'm into invitro methods as well and really have no desire for big tubs of bulk grows and thousands of mushrooms. I'm going to post three really good threads for you on this that you'll get a lot out of. Especially Violet's V-Tek … She's got this method totally dialed in. The Muda Bottle look like they could yield a lot but they involve a fruiting chamber.
http://www.shroomery.org/forums/showflat.php/Number/19035262#19035262
Sclerotia (magic truffles) is totally invitro and this guy is the wizard of sclerotia http://www.shroomery.org/forums/showflat.php/Number/13431948/page/1
http://www.shroomery.org/forums/showflat.php/Number/21168958#21168958
Wow thx man I will check it out, I was wondering if I could use brown rice as substrate cause I cant find RGS where I live and theyre a little expensive on internet but they use rice in your link so I will try it.
You will have to open it up and case it if you use whole grain brown rice, which is no big deal once it's all colonized and the yields are better according to Violet. But, after you case it, you close it up and grow it the way you are now.
Rye grass seed is hard to find? What do people where you live use to seed their lawns?
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: My invitro techinque [Re: KauaiOrca]
#21953471 - 07/16/15 07:38 PM (8 years, 6 months ago) |
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Quote:
KauaiOrca said: I find grows like this absolutely fascinating because obviously, a couple of very small holes with micropore tape on them gives enough FAE for healthy mushrooms to grow. Contrast that with a SGFC, fanning, misting to get the FAE and humidity right. I mean seriously, how much "fresh air" can be getting into that little fruiting chamber?
I would temper that fascination with the understanding of what healthy cubes grown in abundant FAE actually look like. OP has some nice results for what he is attempting to do but the small caps, stretching stipes and fuzzy feet are signs that the FAE was indeed very poor. Compare to the cap sizes that are seen outdoors. I like to use that as a target for how I want my indoor grows to look (tho its really not possible).
Outdoor cubes.

At any rate it does appear like OP has some results. I bet however that he makes a few tweaks as he gains experience with it. Good luck on your future experiments
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KauaiOrca
Waterman


Registered: 08/12/08
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Re: My invitro techinque [Re: Pastywhyte]
#21953545 - 07/16/15 07:57 PM (8 years, 6 months ago) |
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Quote:
Pastywhyte said:
Quote:
KauaiOrca said: I find grows like this absolutely fascinating because obviously, a couple of very small holes with micropore tape on them gives enough FAE for healthy mushrooms to grow. Contrast that with a SGFC, fanning, misting to get the FAE and humidity right. I mean seriously, how much "fresh air" can be getting into that little fruiting chamber?
I would temper that fascination with the understanding of what healthy cubes grown in abundant FAE actually look like. OP has some nice results for what he is attempting to do but the small caps, stretching stipes and fuzzy feet are signs that the FAE was indeed very poor. Compare to the cap sizes that are seen outdoors. I like to use that as a target for how I want my indoor grows to look (tho its really not possible).
Outdoor cubes.

At any rate it does appear like OP has some results. I bet however that he makes a few tweaks as he gains experience with it. Good luck on your future experiments 
Absolutely no doubt you are right and we see pictures day after day of incredible grows with huge mushrooms … Some people really have a touch with it and their constant experimenting and refining generates better and better results.
I think there are a lot of people that have no desire for that level of experimentation and all those tubs and steps, etc. Just want an easy way to grow enough mushrooms for their own use which isn't all that much and Invitro can be a good choice, especially if you find the right culture for it. But, if you want a big grow with lots of big giant mushrooms the way a lot of you guys do it and you're one of the best, then FAE, no doubt is a huge piece of the equation.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: My invitro techinque [Re: KauaiOrca]
#21953562 - 07/16/15 08:05 PM (8 years, 6 months ago) |
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Agreed. Invitro has its merits, I have done a few that way myself
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