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paperbackwriter
Edward Lear


Registered: 03/31/14
Posts: 1,888
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Re: Continual Monotub problems - Cambodian [Re: rudeboi]
#21946267 - 07/15/15 08:15 AM (8 years, 6 months ago) |
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I lost my first tubs to trich.
Now I put everything on agar first. Listen to bodhisatta and spacechildo. They've been patient and have been trying to help you.
-------------------- Why should we strive with cynic frown To knock their fairy castles down? ~ Eliza Cook It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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some people get mad if you say their spawn was dirty. they'd much rather blame the bucket tek or their mail man. never made much sense to me
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Galba Cubensis
azur's handdoll



Registered: 06/16/15
Posts: 173
Loc: Norway
Last seen: 8 years, 5 months
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Re: Continual Monotub problems - Cambodian [Re: spacechildo]
#21946550 - 07/15/15 09:39 AM (8 years, 6 months ago) |
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Sterilize the substrate for 6 hours (that should be sufficient even to sterilize vince vaughn's eye bags;)). If it still shows up after that you can honestly say that there isnt a faintest possibility this is your contam vector. Wouldnt that be a nice feeling? To be comepletely sure beyond any imaginable doubt? I think even 3 might be enough, but hey theres a very mild flame burning here so lets be sure
-------------------- RETARDS! I'LL IGNORE ALL OF YOUR ADVICE! BUT TELL ME WHAT TO DO STILL!
Edited by Galba Cubensis (07/15/15 09:40 AM)
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21946565 - 07/15/15 09:45 AM (8 years, 6 months ago) |
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yeah, try sterilizing manure for 6 hrs to make sure its clean before you spawn to it 
You should fuck off with the advice for now galba.
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Re: Continual Monotub problems - Cambodian [Re: jjgomes]
#21946627 - 07/15/15 10:09 AM (8 years, 6 months ago) |
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Quote:
jjgomes said:
Quote:
tetherface said: maybe try to properly pasteurize your next tub even though they say cvg lacks the beneficial bacteria and micro organisms maybe that will help idk also try to bump up your PC times to 120+ i've read some shit about sterilizing big bags for 3-4 hours on account of how long it takes to get them up to temp i use quarts so i can't say from experience
This kind of happens by default. I always clean the tubs and spray them with bleach. After the time is up on the coir, I dump that into the tub where it cools down.
im not quite sure what your trying to say here the bucket isn't proper pasteurization but like bodhi said trich spores die at around 140 so i doubt its the bucket pasteurization...i really think it was your pc times, you succeeded with smaller bags at 90 min 15psi then on your next batch with much bigger bags 90 min@15psi couldn't quite get it hot enough to sterilize. as a response to your earlier post trich imo is bright white spots with a kind of crystaline structure in between the bright white...
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Galba Cubensis
azur's handdoll



Registered: 06/16/15
Posts: 173
Loc: Norway
Last seen: 8 years, 5 months
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Re: Continual Monotub problems - Cambodian [Re: tetherface]
#21946632 - 07/15/15 10:10 AM (8 years, 6 months ago) |
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The only place I can find anyone mentioning manure is in your post. Maybe you should spend a little more time reading and a little less flaming?
-------------------- RETARDS! I'LL IGNORE ALL OF YOUR ADVICE! BUT TELL ME WHAT TO DO STILL!
Edited by Galba Cubensis (07/15/15 10:11 AM)
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21946640 - 07/15/15 10:13 AM (8 years, 6 months ago) |
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I could be spending way more time helping if I didnt have to clean up your mess 
stop blaming the fucking bucket tek, there's a reason coir+verm can be used non-heat treated for casing layers.
stop fuckin guessing and making claims you dont know squat about.
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Galba Cubensis
azur's handdoll



Registered: 06/16/15
Posts: 173
Loc: Norway
Last seen: 8 years, 5 months
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21946646 - 07/15/15 10:15 AM (8 years, 6 months ago) |
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Again, maybe you should spend a little more time reading... I suggested the coir+verm (called it casing) was to be pasteurized. That was several post ago. Meanwhile weve started focusing on the substrate and pcing.
tl;dr you should read more
-------------------- RETARDS! I'LL IGNORE ALL OF YOUR ADVICE! BUT TELL ME WHAT TO DO STILL!
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21946674 - 07/15/15 10:26 AM (8 years, 6 months ago) |
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well that was terrible advice. you should fuck off with that
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Galba Cubensis
azur's handdoll



Registered: 06/16/15
Posts: 173
Loc: Norway
Last seen: 8 years, 5 months
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21946698 - 07/15/15 10:32 AM (8 years, 6 months ago) |
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So thats the 3rd post not specifying what I said wrong nor what "mess" you have cleaned up... Seems to me its more about the fact that you dont like me and dont want to accept me into your imaginary hirarchy of "trusted knowledge". But Ill bite, could you please school me as to why it was terrible advice? All arguments Ive gathered so far are "theres a reason bla bla works", which ofc I can replicate into "theres a reason bla bla works, until it doesnt, then theres a reason it doesnt"...
-------------------- RETARDS! I'LL IGNORE ALL OF YOUR ADVICE! BUT TELL ME WHAT TO DO STILL!
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21946721 - 07/15/15 10:38 AM (8 years, 6 months ago) |
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if your spawn is dirty wtf does it matter what you do to your sub. especially a sub that doesnt care.
if you really wanna learn more and not just troll make your own thread, listen to what people say (unlike your last thread) and checkout frankhorrigan sbj and tl's writeups.
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Galba Cubensis
azur's handdoll



Registered: 06/16/15
Posts: 173
Loc: Norway
Last seen: 8 years, 5 months
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21946798 - 07/15/15 10:59 AM (8 years, 6 months ago) |
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Ofc I wanna learn, that thread was a mapping thread, you can learn alot about someone/a forum just by looking at how they treat morons;) And so I did, f.ex I learned that you barely have the energy to keep a civilized tone. Dunno if this is maybe cause you spend too much time here, and I really dont care, seeing as its your problem and everyone that listens to your advice based on not reading through the thread.
My first post specifically stated that Im taking into account that he doesnt want to consider his spawn the vector. Well those were the options then... Maybe the verm+coir has a close to zero contamination risk, but that doesnt mean that OP couldnt have contaminated it himself somehow, personally my money were on his clothes being full of spores and cathing a ride along on the outside of the bags cause thats what I experienced 2 weeks ago in the experiments room. The two monotubs wouldve shown wether this is the case, again, keep in mind he didnt want to consider the spawn at first for many many posts....
EDIT: Seems I also need to brush up the reading skills as I also have been able to imagine stuff during this thread, somehow I was under the impression that he was using agar, since somone mentioned it, seems I pulled a spacechildo no?
-------------------- RETARDS! I'LL IGNORE ALL OF YOUR ADVICE! BUT TELL ME WHAT TO DO STILL!
Edited by Galba Cubensis (07/15/15 11:03 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21946807 - 07/15/15 11:00 AM (8 years, 6 months ago) |
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paperbackwriter
Edward Lear


Registered: 03/31/14
Posts: 1,888
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Re: Continual Monotub problems - Cambodian [Re: spacechildo]
#21946830 - 07/15/15 11:08 AM (8 years, 6 months ago) |
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Spores don't germinate on coir. You can pasteurize it, sterilize it, or even just hydrate it and spawn.
OP take your spores to agar. Your syringe or LC or whatever you're using is dirty. If it's happening with two syringes they're both dirty. This is why your spawn is dirty. It's not because you're not PCing correctly. It's because you're injecting it with dirty syringes.
-------------------- Why should we strive with cynic frown To knock their fairy castles down? ~ Eliza Cook It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley
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Galba Cubensis
azur's handdoll



Registered: 06/16/15
Posts: 173
Loc: Norway
Last seen: 8 years, 5 months
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Becuase there arent any nutrients for it to use in the mix right? What if op is handling the casing with bare hands? You think any nutrients/contams might find their way into the stuff hes handling? Or lets say the coir he bought is tainted already from the store? Did you ask him where hes from? I bet coir in france or spain has just the same build up as yours, not to mention the exact same contam vectors and exact same cleaning procedure before it even arrives to the store. So many things to take into consideration, but better to just assume he has the exact same coir as you, since you are the center of the universe:D
-------------------- RETARDS! I'LL IGNORE ALL OF YOUR ADVICE! BUT TELL ME WHAT TO DO STILL!
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Quote:
spacechildo said: if your spawn is dirty wtf does it matter what you do to your sub. especially a sub that doesnt care.
if you really wanna learn more and not just troll make your own thread, listen to what people say (unlike your last thread) and checkout frankhorrigan sbj and tl's writeups.
Quote:
paperbackwriter said: Spores don't germinate on coir. You can pasteurize it, sterilize it, or even just hydrate it and spawn.
OP take your spores to agar. Your syringe or LC or whatever you're using is dirty. If it's happening with two syringes they're both dirty. This is why your spawn is dirty. It's not because you're not PCing correctly. It's because you're injecting it with dirty syringes.
thats what i was thinking but 90 minutes isn't long enough for those big ass bags hes using it will kill the trich but not the bacteria and a bacterial infection causes weak myc which is then easilly taken over by trich after spawning or like we both said its a bad batch of syringes... OP most vendors will replace syringes for a nominal fee if there bad or not the vendor i use charges like 4$ and doesn't question it if i were you i'd go that route which i've done before
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paperbackwriter
Edward Lear


Registered: 03/31/14
Posts: 1,888
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21946905 - 07/15/15 11:34 AM (8 years, 6 months ago) |
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I don't work with bags so you might be right tetherface 
Quote:
Galba Cubensis said: Becuase there arent any nutrients for it to use in the mix right? What if op is handling the casing with bare hands? You think any nutrients/contams might find their way into the stuff hes handling? Or lets say the coir he bought is tainted already from the store? Did you ask him where hes from? I bet coir in france or spain has just the same build up as yours, not to mention the exact same contam vectors and exact same cleaning procedure before it even arrives to the store. So many things to take into consideration, but better to just assume he has the exact same coir as you, since you are the center of the universe:D
I am the center of the universe, thanks for noticing.
-------------------- Why should we strive with cynic frown To knock their fairy castles down? ~ Eliza Cook It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley
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Galba Cubensis
azur's handdoll



Registered: 06/16/15
Posts: 173
Loc: Norway
Last seen: 8 years, 5 months
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Re: Continual Monotub problems - Cambodian [Re: tetherface]
#21946913 - 07/15/15 11:35 AM (8 years, 6 months ago) |
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Quote:
tetherface said: thats what i was thinking but 90 minutes isn't long enough for those big ass bags hes using it will kill the trich but not the bacteria
I found close to nothing on lentils as a substrate, so started experimenting with that. Found out it takes forever to sterilize them, had to repc two small jars yesterday for 2 hours, after the initial one wasnt enough, Small jars! Might be because they werent hydrated good enough. The time it takes for something to sterilize in the pc compared to greater mass/volume seems exponential rather than proportional IME, might be somthing to take into consideration.
paperbackwriter, not you you... you keep them paperbacks written!
-------------------- RETARDS! I'LL IGNORE ALL OF YOUR ADVICE! BUT TELL ME WHAT TO DO STILL!
Edited by Galba Cubensis (07/15/15 11:42 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21947347 - 07/15/15 01:16 PM (8 years, 6 months ago) |
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you can spawn to straw with your bare hands given your spawn is clean. So... If you get trich not from your spawn it is likely to be a small isolated spot. If it's in your spawn you come here and say your tub turned green
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21956219 - 07/17/15 01:38 PM (8 years, 6 months ago) |
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Quote:
Galba Cubensis said:
Quote:
tetherface said: thats what i was thinking but 90 minutes isn't long enough for those big ass bags hes using it will kill the trich but not the bacteria
I found close to nothing on lentils as a substrate, so started experimenting with that. Found out it takes forever to sterilize them, had to repc two small jars yesterday for 2 hours, after the initial one wasnt enough, Small jars! Might be because they werent hydrated good enough. The time it takes for something to sterilize in the pc compared to greater mass/volume seems exponential rather than proportional IME, might be somthing to take into consideration.
paperbackwriter, not you you... you keep them paperbacks written!
so how did you know to re sterilize them?? did you leave them uninnoculated for several days? and are you trying to say that mass/volume doesn't matter when trying to come up with an acceptable PC time for sterilization? IME the bigger it is the longer it will take to sterilize but maybe i misunderstood your post
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