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razerath
Stranger

Registered: 07/13/15
Posts: 13
Last seen: 7 years, 1 month
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Re: Continual Monotub problems - Cambodian [Re: jjgomes]
#21944047 - 07/14/15 09:25 PM (8 years, 6 months ago) |
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LC?
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jjgomes
Stranger

Registered: 05/21/14
Posts: 34
Last seen: 8 years, 6 months
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Re: Continual Monotub problems - Cambodian [Re: tetherface]
#21944057 - 07/14/15 09:28 PM (8 years, 6 months ago) |
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Quote:
tetherface said: put a few drops of each syringe on agar and see what grows but i highly doubt you got 2 bad syringes from 2 diff vendors but i've gotten 3 bad rigs in one order before so it can happen either way trich usually doesnt sporulate until you introduce fruiting conditions do you know what trich looks like before it goes green?(not trying to be a dick) just wanna help
This.
This is information that I didn't have before.
Are you entirely sure? I have seen grain jars contaminated with trich and they are either a dark grey, or they eventually turn green in the jar. Do you have more information on it?
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jjgomes
Stranger

Registered: 05/21/14
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Last seen: 8 years, 6 months
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Re: Continual Monotub problems - Cambodian [Re: razerath]
#21944058 - 07/14/15 09:28 PM (8 years, 6 months ago) |
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Quote:
razerath said: LC?
MS syringe
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razerath
Stranger

Registered: 07/13/15
Posts: 13
Last seen: 7 years, 1 month
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Re: Continual Monotub problems - Cambodian [Re: jjgomes]
#21944077 - 07/14/15 09:32 PM (8 years, 6 months ago) |
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Id keep a LC as a fallback. I would make it first for less contams. My two cents...
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Re: Continual Monotub problems - Cambodian [Re: jjgomes]
#21944130 - 07/14/15 09:46 PM (8 years, 6 months ago) |
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maybe try to properly pasteurize your next tub even though they say cvg lacks the beneficial bacteria and micro organisms maybe that will help idk also try to bump up your PC times to 120+ i've read some shit about sterilizing big bags for 3-4 hours on account of how long it takes to get them up to temp i use quarts so i can't say from experience
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Continual Monotub problems - Cambodian [Re: jjgomes]
#21944380 - 07/14/15 10:42 PM (8 years, 6 months ago) |
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Quote:
jjgomes said: It isn't in the spawn. I do all bags, they are all pressure cooked, 15psi, 90 minutes, they are all fluffy white.
I realize they are the same species, but I have had all of my golden teachers and all of my cambodians colonize at noticably different rates.
Oh ok sorry you know so continue on and teach me how to overcome trich
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jjgomes
Stranger

Registered: 05/21/14
Posts: 34
Last seen: 8 years, 6 months
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Re: Continual Monotub problems - Cambodian [Re: tetherface]
#21944383 - 07/14/15 10:43 PM (8 years, 6 months ago) |
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Quote:
tetherface said: maybe try to properly pasteurize your next tub even though they say cvg lacks the beneficial bacteria and micro organisms maybe that will help idk also try to bump up your PC times to 120+ i've read some shit about sterilizing big bags for 3-4 hours on account of how long it takes to get them up to temp i use quarts so i can't say from experience
This kind of happens by default. I always clean the tubs and spray them with bleach. After the time is up on the coir, I dump that into the tub where it cools down.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Continual Monotub problems - Cambodian [Re: jjgomes]
#21944391 - 07/14/15 10:45 PM (8 years, 6 months ago) |
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 Trich is a parasite of cubes in the right circumstances. A facultative parasite.
Keep being a belligerent noob or buy a scope and find out how fucked your 'clean' spawn is
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jjgomes
Stranger

Registered: 05/21/14
Posts: 34
Last seen: 8 years, 6 months
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Re: Continual Monotub problems - Cambodian [Re: bodhisatta]
#21944402 - 07/14/15 10:47 PM (8 years, 6 months ago) |
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Quote:
bodhisatta said:
Oh ok sorry you know so continue on and teach me how to overcome trich
Nope, I know a little. Just enough to be dangerous. You may know more than me, but you certainly didn't take the time to find out what was going on in my situation.
What I do know is that shroomery members, without gathering adequate information INSTANTLY jump to conclusions and say "you did it wrong," "obviously you messed up," "start all over." Not only is that useless, it is detrimental to people who actually want to find out what went wrong, and where. You do that through methodical testing, not through arrogant and presumptuous comments.
If you want to have a conversation where everyone learns then I am willing to do that. As you can see if you read on, I did say I haven't ruled out that as a possible outcome.
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jjgomes
Stranger

Registered: 05/21/14
Posts: 34
Last seen: 8 years, 6 months
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Re: Continual Monotub problems - Cambodian [Re: bodhisatta]
#21944405 - 07/14/15 10:48 PM (8 years, 6 months ago) |
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Quote:
bodhisatta said:
 Trich is a parasite of cubes in the right circumstances. A facultative parasite.
Keep being a belligerent noob or buy a scope and find out how fucked your 'clean' spawn is
First quality post from you.
Thanks, I am going to research this. This is something I didn't know. Where are these from?
Edited by jjgomes (07/14/15 10:49 PM)
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jjgomes
Stranger

Registered: 05/21/14
Posts: 34
Last seen: 8 years, 6 months
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Re: Continual Monotub problems - Cambodian [Re: tetherface]
#21944439 - 07/14/15 10:57 PM (8 years, 6 months ago) |
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Quote:
tetherface said: maybe try to properly pasteurize your next tub even though they say cvg lacks the beneficial bacteria and micro organisms maybe that will help idk also try to bump up your PC times to 120+ i've read some shit about sterilizing big bags for 3-4 hours on account of how long it takes to get them up to temp i use quarts so i can't say from experience
This is pretty interesting. My earlier attempts did use smaller bags, maybe I do need to keep them in there for additional time.
thanks
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Galba Cubensis
azur's handdoll



Registered: 06/16/15
Posts: 173
Loc: Norway
Last seen: 8 years, 5 months
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Re: Continual Monotub problems - Cambodian [Re: tetherface]
#21944541 - 07/14/15 11:09 PM (8 years, 6 months ago) |
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If you dont wanna consider the spawn a possibility then I have a quick question, does your pc have a pressure indicator? jesuisravi had an interesting point, but I reckon he was using an "old teched" pc. Furthermore whats the chance of you chaning your pc, and then the chance of you not telling us on top? Microscopic I think, so prob not the pc.
Which leaves me to two more solutions. You said you stored the bags a month or two after full colonization, where? Cold they be accumulating trich spores on the outside, that you then "mist" into the air and tub when you case? Btw I was under the impression that its mostly the co2/o2 levels that decide if its gonna fruit?
If all fails and youre still determined to make it work. Then go back to square one and repeat the earlier successfull, superuncontaminated tek you used for the one you talked about that had no problems. Think about it for a couple of days, try to remember as much details of what exactly you did, then repeat it to the letter. See if you still have the problem then, if you cant repeat it then maybe something changed in your enviroment.
-------------------- RETARDS! I'LL IGNORE ALL OF YOUR ADVICE! BUT TELL ME WHAT TO DO STILL!
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jjgomes
Stranger

Registered: 05/21/14
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Last seen: 8 years, 6 months
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21944593 - 07/14/15 11:23 PM (8 years, 6 months ago) |
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Quote:
Galba Cubensis said: If you dont wanna consider the spawn a possibility then I have a quick question, does your pc have a pressure indicator? jesuisravi had an interesting point, but I reckon he was using an "old teched" pc. Furthermore whats the chance of you chaning your pc, and then the chance of you not telling us on top? Microscopic I think, so prob not the pc.
Which leaves me to two more solutions. You said you stored the bags a month or two after full colonization, where? Cold they be accumulating trich spores on the outside, that you then "mist" into the air and tub when you case? Btw I was under the impression that its mostly the co2/o2 levels that decide if its gonna fruit?
If all fails and youre still determined to make it work. Then go back to square one and repeat the earlier successfull, superuncontaminated tek you used for the one you talked about that had no problems. Think about it for a couple of days, try to remember as much details of what exactly you did, then repeat it to the letter. See if you still have the problem then, if you cant repeat it then maybe something changed in your enviroment.
Sigh... I am considering it as an option.
My pressure cooker is the Presto 01781. It has a pressure gauge on it and I am always at or slightly above 15psi. I only start timing once I am at 15psi.
I inoculated a few bags. They all colonized about 2 weeks after first sign of growth. I gave one bag to a friend and his got trich, however his house is a disaster zone so I pretty much expected that to happen. I did two of my own bags and they both got trich. At that point I was just cleaning my tubs with soap and water so I went to town and cleaned up everything with sanitizer. I went out of town for a month so I rolled the bags up and I put them in my closet to stay cool (70ish) and dark while I was gone. After I got back, I started another tub and just saw a patch of grey among the majority of white patch.
While I have cleaned the area, I am growing this in the same room as the old tubs.
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Galba Cubensis
azur's handdoll



Registered: 06/16/15
Posts: 173
Loc: Norway
Last seen: 8 years, 5 months
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Re: Continual Monotub problems - Cambodian [Re: jjgomes]
#21944669 - 07/14/15 11:45 PM (8 years, 6 months ago) |
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Well if electricity/gas isnt a problem try leaving those suckers on for, I dunno, lets say 6 hours ok? That should take any insecurities we might have out of the substrate. You shouldve store it even colder I think, even maybe in the fridge. Likewise with the casing; are you sterilizing it? Dont. Pasteurizing? Do. Nothing? You need to do something!
Heres another solution: Prep two monotubs without any spawn as far away from the room you usually do it in (do you use SAB btw? if not then you might just have been really lucky the first time...), just the casing as you would prep it for spawn. Close one after the casing preparement, take the clothes out of the closet you stored the bags in, unfold them carefully one by one and shake them over the other monotub before you close this one off too. Then wait and see what they get;)
What do you mean "same area as the old tubs"? They werent cleaned? Srsly just stay away from that f place for now....
EDIT: Im assuming youre pasteurizing the casing before putting it into the tubs.
-------------------- RETARDS! I'LL IGNORE ALL OF YOUR ADVICE! BUT TELL ME WHAT TO DO STILL!
Edited by Galba Cubensis (07/15/15 12:16 AM)
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
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Re: Continual Monotub problems - Cambodian [Re: Galba Cubensis]
#21944762 - 07/15/15 12:14 AM (8 years, 6 months ago) |
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For my two cents: I'd say your syringe or whatever inoculation method you are using (I decided not to read all of the flame war) is contaminated. Like, before you put it to spawn. Golden teachers and Cambodians should be virtually the same- especially from MS. Not every single spore in your syringe would be a slo colonizer. That's just too bad of luck, so it has to be that the source itself is contaminated, especially if you are doing both at the same time.
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MagicInMichigan
Internal conquistador



Registered: 07/07/15
Posts: 138
Loc: Michigan,U.S.A.
Last seen: 8 years, 2 months
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Re: Continual Monotub problems - Cambodian [Re: TravelAgency]
#21944894 - 07/15/15 12:54 AM (8 years, 6 months ago) |
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Quote:
TravelAgency said: For my two cents: I'd say your syringe or whatever inoculation method you are using (I decided not to read all of the flame war) is contaminated. Like, before you put it to spawn. Golden teachers and Cambodians should be virtually the same- especially from MS. Not every single spore in your syringe would be a slo colonizer. That's just too bad of luck, so it has to be that the source itself is contaminated, especially if you are doing both at the same time.
What would be the odds of getting two contaminated Cambodian syringes from two different suppliers? I'm going to have to say that maybe with the larger bags its taking longer for the centers to get up to temp and they aren't pasturizing for as long as is needed. Thats the only thing I can think of. Trich just chilling in the center of the spawn that didnt get hot enough quite long enough to kill it. That shit waits to attack, trust me. I've had it pop out from the center of what I though was a full colonized clean monstor quart PF cake. When I broke the contaminated one up it was green in the center and spreading outward. My house is a mold fucking wreck and the still air box has made it damn near impossible for me to get a contam now though. I had gotten lazy and didnt pasteurize long enough as I had a date and a deadline to get everything done in a few hours. Bad idea.
I say find someone with a decent scope and look at the spore solution under it and see if it could be both syringes are contaminated. Maybe some trich spores got inside the needle at inoculation and are chilling up there. I'd thouroughly clean your still air box as well. Could be any of those things really. Good luck man, I hope you get that nasty green bitch under control, it's the green devil.
-MIM
-------------------- “the only people for me are the mad ones, the ones who are mad to live, mad to talk, mad to be saved, desirous of everything at the same time, the ones who never yawn or say a commonplace thing, but burn, burn, burn like fabulous yellow roman candles exploding like spiders across the stars.” I surround myself with people who aren't afraid to live. They have had what they love most taken from them, be it freedom, love, money, or anything for that matter. When you lose your greatest love you also lose your greatest fear, when that happens you are completely and utterly free.
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 23 days
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Yep and might I add I traced all of my conams to poor inoculation technique [which I have no fixed] so... Maybe?) but I really think you got a bad syringe or two. Bad luck, but not as bad of luck as getting 2 syringes full of super slow colonizers. I just don't see that as a probability.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Continual Monotub problems - Cambodian [Re: TravelAgency]
#21945999 - 07/15/15 06:25 AM (8 years, 6 months ago) |
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Even though your attitude is shit, and you didn't sterilize your bags nearly long enough the failure was still you, not insufficient sterilization. Trich would be killed off under pasteurization temps. The spawn bags look perhaps a little bacterial too which never helped anything.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Continual Monotub problems - Cambodian [Re: bodhisatta]
#21946002 - 07/15/15 06:27 AM (8 years, 6 months ago) |
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Or take one fully colonized bag never spawn it to coir just cut some slits for air after full colonization and watch the trich that you don't think is in your spawn turn green in a few days.
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rudeboi
Noob

Registered: 05/17/14
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Loc: North BC
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Re: Continual Monotub problems - Cambodian [Re: bodhisatta]
#21946222 - 07/15/15 08:02 AM (8 years, 6 months ago) |
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After making the move to grow bags last year I had a solid success rate. When I ordered larger bags the problems from hell begun. The small bags I was doing approx 4qts of spawn and in the large ones I ran 10-15qts. Out of 10 large bags I had 1 partial success which cost me hours of time, WBS and electricity.
After narrowing it down and re-reading my notes I found that the increase in size of WBS per bag didn't allow the grain in the centre of the bag to fully sterilize. So I increased the times from 90 min to 120 and then to 180 and I am currently @ a fail rate of 5%. (I used a bad syringe)
To figure this out I took a bag that was infected with mean green which only appeared in the centre top portion of the bag after it was 85% visually colonized. I cut the bag and dissected it halfway through the centre. I found that the green began almost smack dab in the centre. (I did not kneed or shake this bag)
I hope this helps. I narrowed all possibilities and this seemed to be the only one that made sense. After making the changes I have not had any more major bag failures.
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